FastQCFastQC Report
Fri 10 Feb 2017
ERR1632834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417125
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22270.5338927180101888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.4315253221456398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8310.19922085705723702No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA5750.13784836679652382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.12538207971231644No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4930.11818999100988911No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4260.10212765957446808No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4250.10188792328438717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCG405.9311984E-527.7524
GGTATCA2900.025.517241
GTATCAA8450.023.644971
GCCGCTC551.8988934E-523.54545427
GGACCGT551.8988934E-523.5454546
GACCGTT400.001929136323.1257
TCCGGCG502.698104E-422.216
TCGCCGT502.698104E-422.225
CGCCGGC502.698104E-422.214
GTCCGGC603.7189646E-521.58333215
TAGTACT705.089072E-621.1428594
GTGTTAT450.003821783520.5555551
ACCGTGT450.003821783520.5555558
TAGTACC450.003821783520.5555554
CCCCCCT555.1355467E-420.18181836
CGAGCTC1101.7462298E-920.18181828
TTAGACA656.888662E-519.9230774
GTACTAT656.888662E-519.9230771
TGTCCGG656.888662E-519.92307714
AGGACCG500.00702755218.55
Produced by FastQC (version 0.11.5)