##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632834.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417125
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.737229847168116	33.0	33.0	33.0	27.0	33.0
2	31.24351693137549	33.0	33.0	33.0	27.0	33.0
3	31.691373089601438	33.0	33.0	33.0	27.0	33.0
4	31.814120467485765	33.0	33.0	33.0	27.0	33.0
5	31.939011087803415	33.0	33.0	33.0	33.0	33.0
6	34.805056038357804	37.0	37.0	37.0	27.0	37.0
7	34.971241234641894	37.0	37.0	37.0	27.0	37.0
8	35.14441234641894	37.0	37.0	37.0	33.0	37.0
9	35.22775666766557	37.0	37.0	37.0	33.0	37.0
10	35.26277494755769	37.0	37.0	37.0	33.0	37.0
11	35.28567455798621	37.0	37.0	37.0	33.0	37.0
12	35.22767275996404	37.0	37.0	37.0	33.0	37.0
13	35.25408450704225	37.0	37.0	37.0	33.0	37.0
14	35.217980221756065	37.0	37.0	37.0	33.0	37.0
15	35.26266466886425	37.0	37.0	37.0	33.0	37.0
16	35.25249505543902	37.0	37.0	37.0	33.0	37.0
17	35.23671561282589	37.0	37.0	37.0	33.0	37.0
18	35.255544501048846	37.0	37.0	37.0	33.0	37.0
19	35.239000299670366	37.0	37.0	37.0	33.0	37.0
20	35.25198201977825	37.0	37.0	37.0	33.0	37.0
21	35.24600539406653	37.0	37.0	37.0	33.0	37.0
22	35.25929157926281	37.0	37.0	37.0	33.0	37.0
23	35.240589751273596	37.0	37.0	37.0	33.0	37.0
24	35.23445969433623	37.0	37.0	37.0	33.0	37.0
25	35.21878813305364	37.0	37.0	37.0	33.0	37.0
26	35.18408390770153	37.0	37.0	37.0	33.0	37.0
27	35.1247347917291	37.0	37.0	37.0	33.0	37.0
28	35.10749295774648	37.0	37.0	37.0	33.0	37.0
29	35.130323044650886	37.0	37.0	37.0	33.0	37.0
30	35.09733533113575	37.0	37.0	37.0	33.0	37.0
31	35.10204375187294	37.0	37.0	37.0	33.0	37.0
32	35.10660353611028	37.0	37.0	37.0	33.0	37.0
33	35.07658615522925	37.0	37.0	37.0	33.0	37.0
34	35.003085406053344	37.0	37.0	37.0	27.0	37.0
35	34.99458675456997	37.0	37.0	37.0	27.0	37.0
36	34.97147857356907	37.0	37.0	37.0	27.0	37.0
37	35.00391489361702	37.0	37.0	37.0	27.0	37.0
38	34.85861312556188	37.0	37.0	37.0	27.0	37.0
39	34.922337428828286	37.0	37.0	37.0	27.0	37.0
40	34.95935031465388	37.0	37.0	37.0	27.0	37.0
41	34.95597003296374	37.0	37.0	37.0	27.0	37.0
42	34.85506502846869	37.0	37.0	37.0	27.0	37.0
43	34.6346706622715	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	9.0
16	22.0
17	26.0
18	21.0
19	19.0
20	32.0
21	83.0
22	219.0
23	537.0
24	1149.0
25	2147.0
26	3511.0
27	5315.0
28	7350.0
29	10030.0
30	13001.0
31	16511.0
32	20893.0
33	27607.0
34	38450.0
35	69766.0
36	200424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38975127359904	20.703386275097394	13.61030866047348	24.296553790830085
2	15.67395864548996	23.76649685346119	37.754629907102185	22.80491459394666
3	17.948816302067723	27.27096194186395	30.917830386574767	23.862391369493558
4	12.522625112376387	18.031045849565476	40.26586754569973	29.180461492358408
5	13.878094096493857	37.027509739286785	35.20287683548097	13.891519328738388
6	28.554510038957147	40.7522924782739	17.587773449205873	13.105424033563082
7	25.464309259814204	32.875516931375486	23.893077614623913	17.767096194186394
8	24.624992508240933	34.959544501048846	21.461192688043152	18.954270302667066
9	26.176086305064427	14.894815702727	20.56793527120168	38.36116272100689
10	14.673059634402158	28.440635301168715	34.37171111777045	22.514593946658675
11	33.65130356607732	24.02397362900809	24.558345819598443	17.76637698531615
12	22.645489961042852	25.790590350614323	31.04848666466886	20.51543302367396
13	28.70770152831885	22.487264009589452	26.302667066227148	22.50236739586455
14	21.751513335331136	21.385915492957746	28.77051243632005	28.092058735391067
15	23.5428228948157	29.24471081810009	24.83452202577165	22.377944261312557
16	21.972550194785736	28.766916391968834	26.072999700329635	23.18753371291579
17	22.122864848666467	27.758825292178603	26.988552592148636	23.129757267006294
18	22.911357506742583	26.78189991009889	28.524303266406953	21.782439316751574
19	24.426730596344022	26.70590350614324	28.252921786035362	20.614444111477376
20	24.450704225352112	26.345100389571474	27.724543002697033	21.47965238237938
21	23.064309259814202	26.978243931675156	28.08366796523824	21.8737788432724
22	22.5733293377285	27.168594545999404	28.175247228049145	22.082828888222956
23	22.636140245729695	26.820497452801916	28.311657177105182	22.2317051243632
24	22.295474977524723	26.996463889721305	28.183637998201977	22.524423134551995
25	22.919029068025175	27.169553491159725	28.235900509439617	21.675516931375487
26	22.86868444710818	27.017081210668266	27.960683248426733	22.15355109379682
27	22.772789931075817	26.938927180101892	28.26658675456997	22.021696134252323
28	22.60257716511837	27.062391369493554	28.098291878933175	22.2367395864549
29	22.864848666466887	27.31099790230746	27.70967935271202	22.114474078513634
30	23.288462691039857	27.032903805813607	27.653341324543	22.025292178603536
31	22.836080311657177	27.034821696134255	27.775127359904104	22.353970632304467
32	21.818879232843873	27.132154629907102	28.408990110878037	22.63997602637099
33	21.900389571471383	27.10770152831885	28.543961642193587	22.447947258016182
34	22.47935271201678	26.77111177704525	28.16397962241534	22.585555888522624
35	22.2002996703626	27.17938267905304	28.028528618519626	22.591789032064728
36	22.45537908300869	27.13455199280791	28.197782439316754	22.21228648486665
37	22.78381780041954	26.749056038357804	28.212646089301767	22.254480071920888
38	22.841114773748878	26.995984417141145	27.56943362301468	22.593467186095296
39	22.395445010488462	26.802756967335927	28.194665867545698	22.60713215462991
40	22.295474977524723	26.975127359904107	28.350734192388373	22.3786634701828
41	22.34486065328139	26.515552891819	28.28624513035661	22.853341324543003
42	21.982139646388973	27.17698531615223	28.309979023074618	22.530896014384176
43	22.497572670062933	26.635900509439615	28.26203176505844	22.604495055439017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	24.0
2	37.0
3	156.5
4	276.0
5	276.0
6	334.0
7	392.0
8	370.5
9	349.0
10	527.5
11	706.0
12	706.0
13	1065.0
14	1424.0
15	2361.0
16	3298.0
17	3706.0
18	4114.0
19	4114.0
20	3990.0
21	3866.0
22	4549.0
23	5232.0
24	6645.5
25	8059.0
26	8059.0
27	9863.5
28	11668.0
29	13477.0
30	15286.0
31	17050.5
32	18815.0
33	18815.0
34	20730.5
35	22646.0
36	24038.5
37	25431.0
38	26218.5
39	27006.0
40	27006.0
41	27637.0
42	28268.0
43	28517.5
44	28767.0
45	29206.0
46	29645.0
47	29645.0
48	29840.5
49	30036.0
50	29373.0
51	28710.0
52	27876.5
53	27043.0
54	27043.0
55	25136.5
56	23230.0
57	21326.0
58	19422.0
59	18153.5
60	16885.0
61	16885.0
62	14546.5
63	12208.0
64	10463.5
65	8719.0
66	7333.5
67	5948.0
68	5948.0
69	5105.5
70	4263.0
71	3491.0
72	2719.0
73	2036.0
74	1353.0
75	1353.0
76	961.0
77	569.0
78	436.5
79	304.0
80	245.5
81	187.0
82	187.0
83	145.5
84	104.0
85	96.0
86	88.0
87	55.5
88	23.0
89	23.0
90	18.0
91	13.0
92	8.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	417125.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.14706538231232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.59322299688846	66.08182248133225
2	5.095633804281159	7.352700504835874
3	1.145945685593585	2.480298549092973
4	0.5430638678486099	1.5672185752178034
5	0.2905939101098697	1.048274891619928
6	0.21246503909956688	0.9197237440423198
7	0.1408741396775325	0.7114559036194356
8	0.11899203945408324	0.6867941160374746
9	0.08780997725040383	0.570170895291382
>10	0.6684694506540124	9.47307844293157
>50	0.06382940270182802	3.2095887767054396
>100	0.03742875966998153	4.462852180076573
>500	0.001002556062588791	0.4650914022348843
>1k	6.683707083925273E-4	0.9709295369620939
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2227	0.5338927180101888	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1800	0.4315253221456398	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.19922085705723702	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	575	0.13784836679652382	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	523	0.12538207971231644	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	493	0.11818999100988911	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	426	0.10212765957446808	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	425	0.10188792328438717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.79472580161822E-4	0.0	0.0
2	0.0	0.0	9.58945160323644E-4	0.0	0.0
3	0.0	0.0	0.001198681450404555	0.0	0.0
4	0.0	0.0	0.001198681450404555	0.0	0.0
5	0.0	0.0	0.001198681450404555	0.0	0.0
6	0.0	0.0	0.002157626610728199	2.39736290080911E-4	0.0
7	0.0	0.0	0.005513934671860953	2.39736290080911E-4	0.0
8	0.0	0.0	0.00719208870242733	2.39736290080911E-4	0.0
9	0.0	0.0	0.009349715313155529	4.79472580161822E-4	0.0
10	2.39736290080911E-4	0.0	0.010788133053640995	4.79472580161822E-4	0.0
11	2.39736290080911E-4	0.0	0.01678154030566377	4.79472580161822E-4	0.0
12	2.39736290080911E-4	0.0	0.017980221756068324	7.19208870242733E-4	0.0
13	2.39736290080911E-4	0.0	0.019898112076715612	7.19208870242733E-4	0.0
14	2.39736290080911E-4	0.0	0.022055738687443812	7.19208870242733E-4	0.0
15	2.39736290080911E-4	0.0	0.024213365298172012	7.19208870242733E-4	0.0
16	2.39736290080911E-4	0.0	0.025172310458495654	9.58945160323644E-4	0.0
17	2.39736290080911E-4	0.0	0.02589151932873839	9.58945160323644E-4	0.0
18	2.39736290080911E-4	0.0	0.02661072819898112	9.58945160323644E-4	0.0
19	2.39736290080911E-4	0.0	0.02685046448906203	0.001198681450404555	0.0
20	2.39736290080911E-4	0.0	0.027329937069223854	0.001198681450404555	0.0
21	2.39736290080911E-4	0.0	0.028288882229547497	0.001198681450404555	0.0
22	2.39736290080911E-4	0.0	0.028288882229547497	0.001438417740485466	0.0
23	2.39736290080911E-4	0.0	0.02852861851962841	0.001678154030566377	0.0
24	2.39736290080911E-4	0.0	0.02876835480970932	0.002876835480970932	0.0
25	2.39736290080911E-4	0.0	0.02876835480970932	0.003116571771051843	0.0
26	2.39736290080911E-4	0.0	0.02876835480970932	0.003596044351213665	0.0
27	4.79472580161822E-4	0.0	0.02876835480970932	0.004554989511537309	0.0
28	4.79472580161822E-4	0.0	0.02876835480970932	0.016062331435421035	0.0
29	4.79472580161822E-4	0.0	0.02876835480970932	0.044830686245130355	0.0
30	4.79472580161822E-4	0.0	0.02876835480970932	0.07575666766556788	0.0
31	4.79472580161822E-4	0.0	0.02876835480970932	0.158945160323644	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCCG	40	5.9311984E-5	27.75	24
GGTATCA	290	0.0	25.51724	1
GTATCAA	845	0.0	23.64497	1
GCCGCTC	55	1.8988934E-5	23.545454	27
GGACCGT	55	1.8988934E-5	23.545454	6
GACCGTT	40	0.0019291363	23.125	7
TCCGGCG	50	2.698104E-4	22.2	16
TCGCCGT	50	2.698104E-4	22.2	25
CGCCGGC	50	2.698104E-4	22.2	14
GTCCGGC	60	3.7189646E-5	21.583332	15
TAGTACT	70	5.089072E-6	21.142859	4
GTGTTAT	45	0.0038217835	20.555555	1
ACCGTGT	45	0.0038217835	20.555555	8
TAGTACC	45	0.0038217835	20.555555	4
CCCCCCT	55	5.1355467E-4	20.181818	36
CGAGCTC	110	1.7462298E-9	20.181818	28
TTAGACA	65	6.888662E-5	19.923077	4
GTACTAT	65	6.888662E-5	19.923077	1
TGTCCGG	65	6.888662E-5	19.923077	14
AGGACCG	50	0.007027552	18.5	5
>>END_MODULE