FastQCFastQC Report
Fri 10 Feb 2017
ERR1632833.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632833.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307302
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7540.24536124073387092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.1630318058457153No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT3760.12235520758081626No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC3690.12007731807798191No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3140.10217961484142635No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC3110.10120337648306878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG250.00549015629.612
GGTATCA1750.029.61
CTTAGAC451.3201323E-424.6666663
CTGTACC450.003819527320.5555554
GTATCAA4600.020.510871
TATACCA656.88112E-519.9230775
GTCGCCA500.007023443518.512
AATCTAT609.2158397E-418.535
TGCTCGG500.007023443518.510
TAAGGTG752.0610627E-417.2666665
GACCAAT650.001576375417.07692331
CGAGGGT650.001576375417.07692316
CAAATCT1107.761937E-716.81818227
TATACAA1951.8189894E-1216.1282045
GTTCACA700.002586703315.8571421
TTATTCT700.002586703315.8571424
TACACAG700.002586703315.8571425
TATATAC957.0328075E-515.5789483
GCACATC957.0328075E-515.57894815
CTACACA957.0328075E-515.5789484
Produced by FastQC (version 0.11.5)