##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632833.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307302
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603894540224275	33.0	33.0	33.0	27.0	33.0
2	31.15221183070725	33.0	33.0	33.0	27.0	33.0
3	31.59211459736676	33.0	33.0	33.0	27.0	33.0
4	31.731798035808424	33.0	33.0	33.0	27.0	33.0
5	31.832698778400403	33.0	33.0	33.0	33.0	33.0
6	34.70336997481305	37.0	37.0	37.0	27.0	37.0
7	34.876658791677244	37.0	37.0	37.0	27.0	37.0
8	35.034692257128164	37.0	37.0	37.0	33.0	37.0
9	35.129719298930695	37.0	37.0	37.0	33.0	37.0
10	35.178371764583375	37.0	37.0	37.0	33.0	37.0
11	35.17426830935041	37.0	37.0	37.0	33.0	37.0
12	35.149979498994476	37.0	37.0	37.0	33.0	37.0
13	35.181759311686875	37.0	37.0	37.0	33.0	37.0
14	35.14686529863131	37.0	37.0	37.0	33.0	37.0
15	35.176897644662255	37.0	37.0	37.0	33.0	37.0
16	35.154551548639446	37.0	37.0	37.0	33.0	37.0
17	35.137021561851206	37.0	37.0	37.0	33.0	37.0
18	35.140321247502456	37.0	37.0	37.0	33.0	37.0
19	35.134776213626985	37.0	37.0	37.0	33.0	37.0
20	35.15403739643738	37.0	37.0	37.0	33.0	37.0
21	35.134714385197626	37.0	37.0	37.0	33.0	37.0
22	35.1401748117487	37.0	37.0	37.0	33.0	37.0
23	35.13254388191421	37.0	37.0	37.0	33.0	37.0
24	35.107942675283596	37.0	37.0	37.0	33.0	37.0
25	35.10311029540973	37.0	37.0	37.0	33.0	37.0
26	35.057617587910265	37.0	37.0	37.0	33.0	37.0
27	35.02489082401026	37.0	37.0	37.0	27.0	37.0
28	35.00917664056856	37.0	37.0	37.0	27.0	37.0
29	35.030842623868374	37.0	37.0	37.0	27.0	37.0
30	34.993058945272075	37.0	37.0	37.0	27.0	37.0
31	34.98152306200415	37.0	37.0	37.0	27.0	37.0
32	35.01288309220246	37.0	37.0	37.0	27.0	37.0
33	34.989671398168575	37.0	37.0	37.0	27.0	37.0
34	34.923075671489286	37.0	37.0	37.0	27.0	37.0
35	34.92444565931885	37.0	37.0	37.0	27.0	37.0
36	34.89708495226194	37.0	37.0	37.0	27.0	37.0
37	34.93599781322608	37.0	37.0	37.0	27.0	37.0
38	34.78019993361579	37.0	37.0	37.0	27.0	37.0
39	34.86209982362627	37.0	37.0	37.0	27.0	37.0
40	34.905763711267745	37.0	37.0	37.0	27.0	37.0
41	34.913000891631036	37.0	37.0	37.0	27.0	37.0
42	34.84693233366526	37.0	37.0	37.0	27.0	37.0
43	34.61151570767519	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	26.0
17	25.0
18	18.0
19	14.0
20	30.0
21	77.0
22	222.0
23	525.0
24	943.0
25	1825.0
26	2803.0
27	4280.0
28	5721.0
29	7632.0
30	10071.0
31	12747.0
32	15754.0
33	20512.0
34	29582.0
35	52273.0
36	142216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39413996654756	21.14532284202511	13.505281449518714	24.955255741908612
2	15.663093634275077	23.532876453781622	38.103233952268454	22.700795959674846
3	17.625007321787688	27.00600712003176	31.21587233405575	24.1531132241248
4	12.834280284540942	18.30804875985187	39.32092859792647	29.53674235768072
5	13.388132846515804	37.615440185875784	36.203799519690726	12.792627447917685
6	29.250053693109713	39.972404995737094	17.75940280245491	13.018138508698286
7	25.510735367814075	32.742709126527	24.48470885318026	17.261846652478667
8	23.823144658999944	36.79084418584975	21.384175827036596	18.001835328113714
9	25.420596026059055	15.852809288582566	20.478226630480766	38.248368054877616
10	13.946541187496338	29.282269558935507	35.136445581219775	21.634743672348375
11	32.99099908233594	24.163851846066738	24.66921790290984	18.17593116868748
12	21.697222927283256	26.08183480745325	31.382483680548777	20.83845858471471
13	26.988434829581326	22.599917995977897	27.5051903339386	22.906456840502177
14	21.529635342431874	21.616845969111818	27.901543107431774	28.95197558102453
15	23.140754046507993	29.601824914904558	24.814026592732883	22.443394445854565
16	22.519541037806455	29.41894292910557	25.33078209709016	22.73073393599781
17	21.73659787440368	28.760307450000326	26.87291329050901	22.630181385086985
18	21.812419053569453	28.58490995828208	28.11826802298716	21.484402965161305
19	23.720639631372396	27.375676045063162	28.193112963794576	20.71057135976987
20	23.665319457732135	26.999173451523255	28.24648066071812	21.08902643002649
21	22.150848351133412	28.037240239243484	27.399105765663744	22.412805643959363
22	21.806561623419306	27.86379522424195	27.52992170568366	22.799721446655084
23	21.97545085941517	27.92627447917684	28.014461344215135	22.08381331719286
24	22.655238169618162	28.127704993784615	27.06783554939441	22.149221287202817
25	22.499365445067067	27.599234629127047	27.873883020611643	22.02751690519424
26	22.483420218547227	27.65911058177298	27.691651860384898	22.165817339294897
27	22.491230125414088	27.55562931578708	27.145609205276894	22.80753135352194
28	21.988792783646055	27.71963735999115	28.113061418409252	22.178508437953546
29	22.871963085173544	27.860215683594642	27.410495213177917	21.857326018053904
30	22.130672758394024	28.152761778315792	27.575804908526464	22.140760554763716
31	22.7525365926678	27.72354231342458	27.612576553357936	21.91134454054969
32	21.74961438584845	27.69620763939057	27.983872542319933	22.570305432441053
33	22.007341312454848	27.770401754625745	27.99135703640068	22.230899896518732
34	22.26506823906125	27.366889899837943	27.86932724160598	22.498714619494827
35	22.230249070946495	28.01673923371797	27.767147626764547	21.985864068570983
36	22.592758914683277	27.77105258019798	27.38446219028838	22.25172631483036
37	22.676715413502027	27.15016498428256	27.579709861959895	22.593409740255513
38	22.846906300642363	27.524389688319634	27.665944250281484	21.96275976075652
39	22.267020715777964	27.6831911279458	27.75803606875321	22.291752087523022
40	22.12741863053283	27.71020038919369	27.824420277121533	22.33796070315195
41	21.972196731553975	27.484038502840853	28.199946632303075	22.343818133302094
42	22.486999759194536	27.296600738036197	27.607695361566147	22.608704141203116
43	22.420615550826223	26.851436046625142	28.14722976095177	22.580718641596867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	9.5
2	11.0
3	50.5
4	90.0
5	90.0
6	118.5
7	147.0
8	137.5
9	128.0
10	157.5
11	187.0
12	187.0
13	342.0
14	497.0
15	873.5
16	1250.0
17	1566.5
18	1883.0
19	1883.0
20	2006.5
21	2130.0
22	2789.0
23	3448.0
24	4381.0
25	5314.0
26	5314.0
27	6755.5
28	8197.0
29	10146.5
30	12096.0
31	13454.5
32	14813.0
33	14813.0
34	16297.0
35	17781.0
36	19091.5
37	20402.0
38	21836.0
39	23270.0
40	23270.0
41	24151.5
42	25033.0
43	24202.0
44	23371.0
45	23316.0
46	23261.0
47	23261.0
48	22341.5
49	21422.0
50	21553.0
51	21684.0
52	21700.0
53	21716.0
54	21716.0
55	19225.5
56	16735.0
57	14938.0
58	13141.0
59	11107.0
60	9073.0
61	9073.0
62	8111.5
63	7150.0
64	5892.0
65	4634.0
66	3865.5
67	3097.0
68	3097.0
69	2520.0
70	1943.0
71	1687.5
72	1432.0
73	1178.5
74	925.0
75	925.0
76	697.5
77	470.0
78	350.5
79	231.0
80	184.0
81	137.0
82	137.0
83	107.5
84	78.0
85	72.5
86	67.0
87	49.0
88	31.0
89	31.0
90	23.0
91	15.0
92	9.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	307302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.93328966712586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.16667092297355	59.449852322848294
2	5.229316327911517	6.89572056418442
3	1.4217047094665083	2.812130052911169
4	0.7095009269627022	1.871189205461046
5	0.45763920560380467	1.5086829153054513
6	0.30536633115064465	1.2080284059805761
7	0.22189589553426656	1.0241228449344968
8	0.16403615889770856	0.8652354864388244
9	0.14007340047063943	0.8311950079100994
>10	1.0308437025722723	13.770389928278995
>50	0.10825852994639765	4.862244910839991
>100	0.043700690987536574	4.490291360758218
>500	9.93197522444013E-4	0.41091699414842964
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	754	0.24536124073387092	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	501	0.1630318058457153	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	376	0.12235520758081626	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	369	0.12007731807798191	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	314	0.10217961484142635	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	311	0.10120337648306878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.2541278611919217E-4	0.0	0.0
2	0.0	0.0	3.2541278611919217E-4	0.0	0.0
3	0.0	0.0	3.2541278611919217E-4	0.0	0.0
4	0.0	0.0	3.2541278611919217E-4	0.0	0.0
5	0.0	0.0	3.2541278611919217E-4	0.0	0.0
6	0.0	0.0	3.2541278611919217E-4	0.0	0.0
7	0.0	0.0	9.762383583575766E-4	0.0	0.0
8	0.0	0.0	9.762383583575766E-4	0.0	0.0
9	0.0	0.0	0.0013016511444767687	3.2541278611919217E-4	0.0
10	0.0	0.0	0.0013016511444767687	6.508255722383843E-4	0.0
11	0.0	0.0	0.0022778895028343453	6.508255722383843E-4	0.0
12	0.0	0.0	0.0022778895028343453	6.508255722383843E-4	0.0
13	0.0	0.0	0.0022778895028343453	6.508255722383843E-4	0.0
14	0.0	0.0	0.0022778895028343453	6.508255722383843E-4	0.0
15	0.0	0.0	0.0026033022889535374	6.508255722383843E-4	0.0
16	0.0	0.0	0.0026033022889535374	9.762383583575766E-4	0.0
17	0.0	0.0	0.0026033022889535374	9.762383583575766E-4	0.0
18	0.0	0.0	0.0026033022889535374	9.762383583575766E-4	0.0
19	0.0	0.0	0.0026033022889535374	9.762383583575766E-4	0.0
20	0.0	0.0	0.0026033022889535374	0.0013016511444767687	0.0
21	0.0	0.0	0.003254127861191922	0.0013016511444767687	0.0
22	0.0	0.0	0.003579540647311114	0.0013016511444767687	0.0
23	0.0	0.0	0.003579540647311114	0.001627063930595961	0.0
24	0.0	0.0	0.003579540647311114	0.0019524767167151532	0.0
25	0.0	0.0	0.003579540647311114	0.0019524767167151532	0.0
26	0.0	0.0	0.003579540647311114	0.0019524767167151532	0.0
27	0.0	0.0	0.003579540647311114	0.003579540647311114	0.0
28	0.0	0.0	0.003579540647311114	0.006833668508503036	0.0
29	0.0	0.0	0.003579540647311114	0.01789770323655557	0.0
30	0.0	0.0	0.003579540647311114	0.03677164483146872	0.0
31	0.0	0.0	0.003579540647311114	0.0872106266799435	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	25	0.005490156	29.6	12
GGTATCA	175	0.0	29.6	1
CTTAGAC	45	1.3201323E-4	24.666666	3
CTGTACC	45	0.0038195273	20.555555	4
GTATCAA	460	0.0	20.51087	1
TATACCA	65	6.88112E-5	19.923077	5
GTCGCCA	50	0.0070234435	18.5	12
AATCTAT	60	9.2158397E-4	18.5	35
TGCTCGG	50	0.0070234435	18.5	10
TAAGGTG	75	2.0610627E-4	17.266666	5
GACCAAT	65	0.0015763754	17.076923	31
CGAGGGT	65	0.0015763754	17.076923	16
CAAATCT	110	7.761937E-7	16.818182	27
TATACAA	195	1.8189894E-12	16.128204	5
GTTCACA	70	0.0025867033	15.857142	1
TTATTCT	70	0.0025867033	15.857142	4
TACACAG	70	0.0025867033	15.857142	5
TATATAC	95	7.0328075E-5	15.578948	3
GCACATC	95	7.0328075E-5	15.578948	15
CTACACA	95	7.0328075E-5	15.578948	4
>>END_MODULE