FastQCFastQC Report
Fri 10 Feb 2017
ERR1632832.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632832.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328791
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9130.27768399986617637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.20529759026250718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3500.10645060235833705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT250.005490707229.66
GGTATCA1950.026.5641041
CCTTGCG400.001928271523.12533
TTATGCG502.6964195E-422.24
TAGACCT603.715878E-521.5833344
GTATATA603.715878E-521.5833341
GTACCTA450.00382008720.5555551
GGCTTAT909.426731E-820.5555551
GTATCAA4150.020.0602421
TATAAGC759.2328155E-619.7333344
GGGTAAA500.00702446318.51
GAATATA500.00702446318.51
CAGGGGA609.2177046E-418.54
GTGTATA902.1427204E-618.51
GTATATT801.6109565E-518.51
GTATTAG1156.3637344E-817.6956521
ATTCAAC852.713552E-517.4117646
TATACAC852.713552E-517.4117643
ATACACT852.713552E-517.4117644
ACTGCAC752.0616155E-417.2666669
Produced by FastQC (version 0.11.5)