##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632832.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328791
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.728633083022345	33.0	33.0	33.0	27.0	33.0
2	31.246880236989455	33.0	33.0	33.0	27.0	33.0
3	31.692102277738744	33.0	33.0	33.0	27.0	33.0
4	31.83364204008017	33.0	33.0	33.0	27.0	33.0
5	31.92606245304768	33.0	33.0	33.0	33.0	33.0
6	34.8324437104422	37.0	37.0	37.0	27.0	37.0
7	34.999425166747265	37.0	37.0	37.0	33.0	37.0
8	35.17075589052012	37.0	37.0	37.0	33.0	37.0
9	35.238105665909345	37.0	37.0	37.0	33.0	37.0
10	35.29771192033845	37.0	37.0	37.0	33.0	37.0
11	35.30528207888902	37.0	37.0	37.0	33.0	37.0
12	35.255204674093875	37.0	37.0	37.0	33.0	37.0
13	35.30069862009605	37.0	37.0	37.0	33.0	37.0
14	35.262409859150644	37.0	37.0	37.0	33.0	37.0
15	35.288979929499284	37.0	37.0	37.0	33.0	37.0
16	35.27356892372358	37.0	37.0	37.0	33.0	37.0
17	35.26191106204245	37.0	37.0	37.0	33.0	37.0
18	35.26861136709947	37.0	37.0	37.0	33.0	37.0
19	35.27706354492672	37.0	37.0	37.0	33.0	37.0
20	35.27485545528923	37.0	37.0	37.0	33.0	37.0
21	35.28490439215185	37.0	37.0	37.0	33.0	37.0
22	35.29085650154658	37.0	37.0	37.0	33.0	37.0
23	35.273343856735735	37.0	37.0	37.0	33.0	37.0
24	35.26583756854659	37.0	37.0	37.0	33.0	37.0
25	35.270509229267226	37.0	37.0	37.0	33.0	37.0
26	35.22743931555304	37.0	37.0	37.0	33.0	37.0
27	35.167008829317105	37.0	37.0	37.0	33.0	37.0
28	35.143051360894916	37.0	37.0	37.0	33.0	37.0
29	35.179381430756926	37.0	37.0	37.0	33.0	37.0
30	35.14064253583584	37.0	37.0	37.0	33.0	37.0
31	35.15235210209525	37.0	37.0	37.0	33.0	37.0
32	35.17835342208272	37.0	37.0	37.0	33.0	37.0
33	35.15567944378039	37.0	37.0	37.0	33.0	37.0
34	35.08603641827179	37.0	37.0	37.0	33.0	37.0
35	35.08509661152525	37.0	37.0	37.0	33.0	37.0
36	35.04775678166373	37.0	37.0	37.0	27.0	37.0
37	35.11020982934448	37.0	37.0	37.0	33.0	37.0
38	34.949916512313294	37.0	37.0	37.0	27.0	37.0
39	35.03307572287562	37.0	37.0	37.0	27.0	37.0
40	35.05535735467212	37.0	37.0	37.0	27.0	37.0
41	35.08731382550009	37.0	37.0	37.0	33.0	37.0
42	35.00753670264697	37.0	37.0	37.0	27.0	37.0
43	34.79684967045935	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	26.0
17	21.0
18	17.0
19	13.0
20	25.0
21	63.0
22	166.0
23	423.0
24	860.0
25	1582.0
26	2544.0
27	3998.0
28	5569.0
29	7612.0
30	9870.0
31	12686.0
32	16147.0
33	21245.0
34	29996.0
35	55600.0
36	160320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.9320814742496	20.75665088156306	13.016475511799289	25.29479213238805
2	16.210906016283904	23.05902533828481	37.2026606567698	23.52740798866149
3	17.988022786511795	26.03173444528591	30.455517334720234	25.52472543348206
4	12.705335608334778	17.416535124136608	39.29304634250938	30.585082925019236
5	13.881766836683484	36.930147114732456	35.61168036837991	13.576405680204143
6	30.073815889121054	39.844460462725564	17.175044329072268	12.90667931908112
7	26.62481637271093	32.18275439412879	23.530449434443156	17.661979798717116
8	24.7230003254347	35.579136898516076	20.870400953797397	18.827461822251827
9	25.768345240593572	15.04664057106186	20.472275700977217	38.71273848736735
10	14.498572041205508	28.48861434771633	34.39966422438571	22.61314938669246
11	34.15726099558686	23.22174268760398	24.54598818094169	18.075008135867463
12	22.65329647101046	25.49066124072739	30.87767000921558	20.978372279046567
13	27.90100702269831	22.03801198937927	26.307897722261252	23.753083265661164
14	21.812032567801428	20.839682351402562	27.548199312024963	29.800085768771044
15	23.451067699541653	29.19818364857919	24.204433819660515	23.146314832218643
16	23.150877000891143	28.50138841999933	25.021974445772543	23.325760133336985
17	22.417584422931284	27.74346013120797	26.645498204026264	23.193457241834476
18	22.8701515552433	27.49831960120563	27.520522155411797	22.111006688139273
19	24.09129203658251	26.789662734077268	27.147336758001284	21.971708471338935
20	23.609222880188327	26.81703574611227	27.357196516936288	22.21654485676311
21	23.032868904562473	26.876343938854774	27.585000805983135	22.50578635059962
22	22.65207989269779	27.139428998968953	27.40342649281763	22.805064615515633
23	22.818142832376797	26.699635938939934	27.5460702999778	22.93615092870547
24	22.609803796332624	27.207557384478285	26.726096517240432	23.456542301948655
25	23.277097000830317	26.981577962900445	27.183834107381283	22.557490928887955
26	23.381722735719652	27.05092292672245	27.02689550504728	22.54045883251062
27	22.763092663728628	27.272644324205952	26.813081866596107	23.15118114546931
28	22.540154687932457	26.88151439668361	27.665599119197303	22.912731796186637
29	23.402404567034985	26.730050396756607	26.991614733979947	22.87593030222847
30	23.103430446697143	26.940214300269776	27.18413825195945	22.77221700107363
31	23.283179892393647	26.840454878631103	27.33681883019912	22.53954639877612
32	22.596725579471457	26.991614733979947	27.350505336216624	23.061154350331975
33	22.67154514570046	26.78783786660827	27.80945950467014	22.73115748302113
34	23.292304229738647	26.525969384806764	27.425324902445624	22.756401483008965
35	23.09004808525781	27.093199023087617	27.407988661490123	22.40876423016445
36	23.61348090428266	26.54360977034043	27.054572661660448	22.788336663716464
37	23.205623024961145	26.378459264395925	27.22124389049579	23.194673820147145
38	23.17034225389381	26.835892709958607	27.06977989056878	22.9239851455788
39	22.711996374596627	26.857486975008438	27.381528083189625	23.048988567205306
40	22.77039213360463	27.29058885431779	27.44691916749546	22.49209984458212
41	22.94071309737797	26.34226605959409	27.547286878290468	23.16973396473748
42	22.872888856446803	26.71575560158277	27.452697914480627	22.958657627489803
43	22.933109482923804	26.253759987347586	27.59382099874997	23.219309530978645
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	12.5
2	19.0
3	69.0
4	119.0
5	119.0
6	134.5
7	150.0
8	138.5
9	127.0
10	170.0
11	213.0
12	213.0
13	364.0
14	515.0
15	904.0
16	1293.0
17	1569.0
18	1845.0
19	1845.0
20	1876.5
21	1908.0
22	2308.0
23	2708.0
24	3580.5
25	4453.0
26	4453.0
27	5969.5
28	7486.0
29	8760.0
30	10034.0
31	11648.5
32	13263.0
33	13263.0
34	15309.0
35	17355.0
36	18914.5
37	20474.0
38	21832.5
39	23191.0
40	23191.0
41	24099.0
42	25007.0
43	25796.0
44	26585.0
45	26304.5
46	26024.0
47	26024.0
48	25413.0
49	24802.0
50	24534.5
51	24267.0
52	23260.0
53	22253.0
54	22253.0
55	20666.0
56	19079.0
57	16928.0
58	14777.0
59	13775.0
60	12773.0
61	12773.0
62	11367.0
63	9961.0
64	8355.0
65	6749.0
66	5601.5
67	4454.0
68	4454.0
69	3594.0
70	2734.0
71	2232.5
72	1731.0
73	1413.0
74	1095.0
75	1095.0
76	815.0
77	535.0
78	418.5
79	302.0
80	254.5
81	207.0
82	207.0
83	160.5
84	114.0
85	107.0
86	100.0
87	73.5
88	47.0
89	47.0
90	36.5
91	26.0
92	17.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	328791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.71984685869556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.54297845313135	59.28397094400141
2	6.808748057793404	9.221747515464203
3	2.013332103490324	4.090276251721804
4	0.9712807995025663	2.630999479964207
5	0.5728863920188526	1.9397889367473664
6	0.37998847151152165	1.5439656659298029
7	0.28599240095239636	1.355715311667286
8	0.1889525512217108	1.0236670265835266
9	0.15724097217881097	0.958350110027534
>10	0.9936813491918273	12.596365513752094
>50	0.06368883675541906	2.9303917335279333
>100	0.020326224496404734	1.93901390384443
>500	9.033877553957659E-4	0.4857476067684106
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	913	0.27768399986617637	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	675	0.20529759026250718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	350	0.10645060235833705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	6.082891563333546E-4	3.041445781666773E-4	0.0
8	0.0	0.0	6.082891563333546E-4	3.041445781666773E-4	0.0
9	0.0	0.0	6.082891563333546E-4	6.082891563333546E-4	0.0
10	0.0	0.0	9.124337345000319E-4	6.082891563333546E-4	0.0
11	0.0	0.0	0.0015207228908333866	6.082891563333546E-4	0.0
12	0.0	0.0	0.0018248674690000638	6.082891563333546E-4	0.0
13	0.0	0.0	0.002129012047166741	6.082891563333546E-4	0.0
14	0.0	0.0	0.002737301203500096	6.082891563333546E-4	0.0
15	0.0	0.0	0.0030414457816667732	6.082891563333546E-4	0.0
16	0.0	0.0	0.0033455903598334506	6.082891563333546E-4	0.0
17	0.0	0.0	0.0036497349380001276	6.082891563333546E-4	0.0
18	0.0	0.0	0.003953879516166805	6.082891563333546E-4	0.0
19	0.0	0.0	0.003953879516166805	6.082891563333546E-4	0.0
20	0.0	0.0	0.003953879516166805	9.124337345000319E-4	0.0
21	0.0	0.0	0.004258024094333482	0.0015207228908333866	0.0
22	0.0	0.0	0.004258024094333482	0.002129012047166741	0.0
23	0.0	0.0	0.004258024094333482	0.0024331566253334184	0.0
24	0.0	0.0	0.004258024094333482	0.002737301203500096	0.0
25	0.0	0.0	0.004258024094333482	0.002737301203500096	0.0
26	0.0	0.0	0.004258024094333482	0.002737301203500096	0.0
27	0.0	0.0	0.004258024094333482	0.0030414457816667732	0.0
28	0.0	0.0	0.004258024094333482	0.01125334939216706	0.0
29	0.0	0.0	0.004258024094333482	0.022202554206167444	0.0
30	0.0	0.0	0.004258024094333482	0.04379681925600153	0.0
31	0.0	0.0	0.004258024094333482	0.09580554212250335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	25	0.0054907072	29.6	6
GGTATCA	195	0.0	26.564104	1
CCTTGCG	40	0.0019282715	23.125	33
TTATGCG	50	2.6964195E-4	22.2	4
TAGACCT	60	3.715878E-5	21.583334	4
GTATATA	60	3.715878E-5	21.583334	1
GTACCTA	45	0.003820087	20.555555	1
GGCTTAT	90	9.426731E-8	20.555555	1
GTATCAA	415	0.0	20.060242	1
TATAAGC	75	9.2328155E-6	19.733334	4
GGGTAAA	50	0.007024463	18.5	1
GAATATA	50	0.007024463	18.5	1
CAGGGGA	60	9.2177046E-4	18.5	4
GTGTATA	90	2.1427204E-6	18.5	1
GTATATT	80	1.6109565E-5	18.5	1
GTATTAG	115	6.3637344E-8	17.695652	1
ATTCAAC	85	2.713552E-5	17.411764	6
TATACAC	85	2.713552E-5	17.411764	3
ATACACT	85	2.713552E-5	17.411764	4
ACTGCAC	75	2.0616155E-4	17.266666	9
>>END_MODULE