FastQCFastQC Report
Fri 10 Feb 2017
ERR1632831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425302
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22750.5349140140417868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.43404451425104984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8170.19209879097676474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.12391194962638313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAT405.931466E-527.75000230
CCGGTTA358.8601717E-426.42857236
GGTATCA2500.025.91
GTATCAA8350.024.5928131
ACGCTTC400.001929197823.12500225
TAGGATC502.6982257E-422.1999994
GCCGGCA1059.767973E-1021.14285915
GCCGGTT450.00382190620.55555535
GCGGGTA450.00382190620.55555522
ATAGTCC450.00382190620.5555553
TTCTGCG450.00382190620.55555518
TCTGCGG555.1357754E-420.18181819
TGTACTA555.1357754E-420.1818185
CGGGGTG759.24328E-619.73333417
CTACACT951.6699232E-719.4736844
CCCACGA1153.0340743E-919.30434819
GGCGAAT701.2169068E-418.532
CTAATAC1103.8346116E-818.53
CGGTTAT500.007027775518.49999837
ACCTTAG500.007027775518.4999981
Produced by FastQC (version 0.11.5)