##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632831.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425302
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.759248722084543	33.0	33.0	33.0	27.0	33.0
2	31.27212898128859	33.0	33.0	33.0	27.0	33.0
3	31.71846123460506	33.0	33.0	33.0	27.0	33.0
4	31.835573310259534	33.0	33.0	33.0	27.0	33.0
5	31.945655087443747	33.0	33.0	33.0	33.0	33.0
6	34.81425904416156	37.0	37.0	37.0	27.0	37.0
7	34.93973223732783	37.0	37.0	37.0	27.0	37.0
8	35.1489012513461	37.0	37.0	37.0	33.0	37.0
9	35.197617222585365	37.0	37.0	37.0	33.0	37.0
10	35.26784731790586	37.0	37.0	37.0	33.0	37.0
11	35.301327057008905	37.0	37.0	37.0	33.0	37.0
12	35.20499080653277	37.0	37.0	37.0	33.0	37.0
13	35.26063126907468	37.0	37.0	37.0	33.0	37.0
14	35.21781228397703	37.0	37.0	37.0	33.0	37.0
15	35.272566317581386	37.0	37.0	37.0	33.0	37.0
16	35.25585583890976	37.0	37.0	37.0	33.0	37.0
17	35.24884435060263	37.0	37.0	37.0	33.0	37.0
18	35.21775115094685	37.0	37.0	37.0	33.0	37.0
19	35.24659183356768	37.0	37.0	37.0	33.0	37.0
20	35.14836045915608	37.0	37.0	37.0	33.0	37.0
21	35.24237365448552	37.0	37.0	37.0	33.0	37.0
22	35.24975194097371	37.0	37.0	37.0	33.0	37.0
23	35.2381178550771	37.0	37.0	37.0	33.0	37.0
24	35.231489623843764	37.0	37.0	37.0	33.0	37.0
25	35.244985915890354	37.0	37.0	37.0	33.0	37.0
26	35.1733779761205	37.0	37.0	37.0	33.0	37.0
27	35.12984655609426	37.0	37.0	37.0	33.0	37.0
28	35.128076049489536	37.0	37.0	37.0	33.0	37.0
29	35.157210640909284	37.0	37.0	37.0	33.0	37.0
30	35.12480072983433	37.0	37.0	37.0	33.0	37.0
31	35.105379236401426	37.0	37.0	37.0	33.0	37.0
32	35.13129023611457	37.0	37.0	37.0	33.0	37.0
33	35.09910604699719	37.0	37.0	37.0	33.0	37.0
34	35.017093735745426	37.0	37.0	37.0	27.0	37.0
35	35.01987293734805	37.0	37.0	37.0	27.0	37.0
36	34.98470028356321	37.0	37.0	37.0	27.0	37.0
37	35.02397590418103	37.0	37.0	37.0	27.0	37.0
38	34.8633371110411	37.0	37.0	37.0	27.0	37.0
39	34.9305340675567	37.0	37.0	37.0	27.0	37.0
40	34.9634777170105	37.0	37.0	37.0	27.0	37.0
41	34.97839652764389	37.0	37.0	37.0	27.0	37.0
42	34.83680302467423	37.0	37.0	37.0	27.0	37.0
43	34.644186954211364	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	9.0
16	27.0
17	26.0
18	18.0
19	14.0
20	27.0
21	75.0
22	250.0
23	563.0
24	1118.0
25	2102.0
26	3415.0
27	5235.0
28	7506.0
29	10039.0
30	13114.0
31	16998.0
32	21598.0
33	27947.0
34	40122.0
35	72545.0
36	202552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.71765474886081	20.717748799676468	13.790671099595112	24.773925351867614
2	15.737522983668075	23.432290466539072	37.698623566312875	23.131562983479974
3	17.69989325232423	27.09745075264165	30.595905967994508	24.60675002703961
4	12.767163098221971	18.02248755002328	39.35909071671424	29.851258635040512
5	14.025798138734357	37.06965873661539	34.99654363252465	13.907999492125594
6	29.02196556799639	40.0148600288736	17.611485485607876	13.351688917522136
7	26.120027650939804	32.28341272789688	23.979666213655236	17.616893407508076
8	24.588645245025887	35.60434702869961	20.8383689707549	18.968638755519606
9	26.164231534297983	14.995226921105473	20.49085120690709	38.34969033768945
10	14.858382984326433	28.284842300294848	34.17171797922418	22.685056736154543
11	33.791047302857734	23.851992231402626	24.455563340873073	17.901397124866566
12	22.50306840786077	25.692566693784652	30.764962309135623	21.039402589218955
13	28.428034667130653	22.101001170932655	26.32035588828644	23.150608273650253
14	22.07114003696197	20.889626665287256	28.15881420731621	28.88041909043456
15	23.668358013834876	28.81223224908418	24.579475290499456	22.93993444658149
16	22.634739549778747	28.25592167448072	25.81224635670653	23.297092419034005
17	22.115814174398427	27.582517834385918	26.956374529158104	23.34529346205755
18	23.147786749180582	26.78049950388195	27.936854282368763	22.134859464568706
19	24.229371129221118	26.58393329916154	27.924392549294385	21.26230302232296
20	23.89525560660425	26.3758458695233	27.733939647591594	21.994958876280855
21	22.978260153961187	26.86749650836347	27.771559973853872	22.382683363821474
22	22.704572280403102	26.63660175592873	27.98246892796178	22.676357035706392
23	22.782869584436472	26.49129324574067	28.13788790083282	22.587949268990034
24	22.6236885789392	26.85621041048479	27.70125698915124	22.818844021424773
25	22.99001650591815	26.63001819883283	28.272850821298746	22.107114473950276
26	22.88256344903151	26.786847933938706	27.906287767280663	22.42430084974912
27	22.772759121753484	26.912170645799925	27.72900197976967	22.58606825267692
28	22.441229996567145	26.921575727365493	28.11625621323201	22.52093806283535
29	22.869396334839713	26.865615492050352	27.705254148816604	22.559734024293327
30	23.131562983479974	26.782850774273342	27.758392859662074	22.32719338258461
31	23.03304475408063	26.612383670897387	27.823993303581922	22.53057827144006
32	22.321785460684406	26.722423125214554	27.910284926946026	23.04550648715501
33	22.509416837917527	26.56982567681318	28.286488189568825	22.634269295700467
34	22.741487225547964	26.35280341968766	27.918984627394185	22.9867247273702
35	22.658957634810086	26.779794122764528	27.950256523599705	22.610991718825684
36	23.04856313866382	26.849391726349747	27.512920230800702	22.58912490418573
37	22.93005911093764	26.32882046169545	27.83245787699094	22.90866255037597
38	22.631682898269936	26.829641055062048	27.5434867458888	22.995189300779213
39	22.329309525936864	26.531735096472627	28.111788799488362	23.027166578102147
40	22.450635078132716	26.615205195367057	28.36737189103273	22.5667878354675
41	22.578544187424466	26.408998782041937	27.97400435455277	23.03845267598083
42	22.3361282100719	26.96742549999765	27.937324536447043	22.75912175348341
43	22.665306064866847	26.220897150730536	28.254275785206744	22.859520999195865
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	35.5
2	57.0
3	193.5
4	330.0
5	330.0
6	395.5
7	461.0
8	438.5
9	416.0
10	556.5
11	697.0
12	697.0
13	1065.0
14	1433.0
15	2352.0
16	3271.0
17	3611.0
18	3951.0
19	3951.0
20	3589.0
21	3227.0
22	3467.0
23	3707.0
24	4838.5
25	5970.0
26	5970.0
27	7400.5
28	8831.0
29	10888.5
30	12946.0
31	14758.5
32	16571.0
33	16571.0
34	19107.0
35	21643.0
36	23428.0
37	25213.0
38	27135.5
39	29058.0
40	29058.0
41	30910.5
42	32763.0
43	33409.0
44	34055.0
45	33885.0
46	33715.0
47	33715.0
48	33434.0
49	33153.0
50	32308.5
51	31464.0
52	29615.0
53	27766.0
54	27766.0
55	25912.5
56	24059.0
57	21474.0
58	18889.0
59	17449.0
60	16009.0
61	16009.0
62	14093.5
63	12178.0
64	10086.0
65	7994.0
66	6834.0
67	5674.0
68	5674.0
69	4788.0
70	3902.0
71	3199.5
72	2497.0
73	2009.5
74	1522.0
75	1522.0
76	1188.0
77	854.0
78	637.0
79	420.0
80	336.0
81	252.0
82	252.0
83	209.5
84	167.0
85	131.5
86	96.0
87	73.0
88	50.0
89	50.0
90	36.5
91	23.0
92	13.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	425302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.60848515366956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.57318775657423	60.768722372101095
2	6.480400343157405	8.892209014667001
3	1.7286008953274374	3.5579006659107724
4	0.8177617441415647	2.2442157792870185
5	0.49050176419035185	1.6826291503151243
6	0.3262954737669395	1.3431982900589188
7	0.24618674978982938	1.182334997758997
8	0.17043881834197736	0.935483931025963
9	0.14890230218976114	0.9194365250220101
>10	0.931940040146881	11.871689004701281
>50	0.05994552433292591	2.8089757397907293
>100	0.024460530011916493	2.5014633308965832
>500	6.890290144201829E-4	0.3176761520103003
>1k	6.890290144201829E-4	0.974065046454202
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2275	0.5349140140417868	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1846	0.43404451425104984	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.19209879097676474	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	527	0.12391194962638313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.3512703913924692E-4	0.0	0.0
2	2.3512703913924692E-4	0.0	4.7025407827849385E-4	0.0	0.0
3	2.3512703913924692E-4	0.0	7.053811174177408E-4	0.0	0.0
4	2.3512703913924692E-4	0.0	0.0011756351956962346	0.0	0.0
5	2.3512703913924692E-4	0.0	0.0018810163131139754	0.0	0.0
6	2.3512703913924692E-4	0.0	0.003762032626227951	0.0	0.0
7	2.3512703913924692E-4	0.0	0.010345589722126866	2.3512703913924692E-4	0.0
8	2.3512703913924692E-4	0.0	0.013402241230937076	2.3512703913924692E-4	0.0
9	2.3512703913924692E-4	0.0	0.016694019778886533	4.7025407827849385E-4	0.0
10	0.0011756351956962346	0.0	0.019045290170279002	4.7025407827849385E-4	0.0
11	0.0011756351956962346	0.0	0.026569355422734902	4.7025407827849385E-4	0.0
12	0.0011756351956962346	0.0	0.03080164212724135	4.7025407827849385E-4	0.0
13	0.0011756351956962346	0.0	0.033152912518633816	7.053811174177408E-4	0.0
14	0.0011756351956962346	0.0	0.035269055870887044	7.053811174177408E-4	0.0
15	0.0011756351956962346	0.0	0.041617485927646705	7.053811174177408E-4	0.0
16	0.0011756351956962346	0.0	0.04584977263215315	7.053811174177408E-4	0.0
17	0.0011756351956962346	0.0	0.047025407827849385	7.053811174177408E-4	0.0
18	0.0011756351956962346	0.0	0.047965915984406374	9.405081565569877E-4	0.0
19	0.0014107622348354817	0.0	0.048671297101824114	9.405081565569877E-4	0.0
20	0.0014107622348354817	0.0	0.049376678219241854	0.0011756351956962346	0.0
21	0.0014107622348354817	0.0	0.05125769453235583	0.0014107622348354817	0.0
22	0.0014107622348354817	0.0	0.05125769453235583	0.0018810163131139754	0.0
23	0.0014107622348354817	0.0	0.051727948610634324	0.00305665150881021	0.0
24	0.0014107622348354817	0.0	0.05219820268891282	0.003762032626227951	0.0
25	0.0014107622348354817	0.0	0.05219820268891282	0.004467413743645692	0.0
26	0.0014107622348354817	0.0	0.052433329728052064	0.004937667821924186	0.0
27	0.0014107622348354817	0.0	0.05266845676719131	0.006818684135038161	0.0
28	0.0014107622348354817	0.0	0.053138710845469804	0.02139656056167147	0.0
29	0.0014107622348354817	0.0	0.053138710845469804	0.047730788945267125	0.0
30	0.0014107622348354817	0.0	0.05337383788460905	0.07923781218992622	0.0
31	0.0014107622348354817	0.0	0.05337383788460905	0.16976172225853628	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAAT	40	5.931466E-5	27.750002	30
CCGGTTA	35	8.8601717E-4	26.428572	36
GGTATCA	250	0.0	25.9	1
GTATCAA	835	0.0	24.592813	1
ACGCTTC	40	0.0019291978	23.125002	25
TAGGATC	50	2.6982257E-4	22.199999	4
GCCGGCA	105	9.767973E-10	21.142859	15
GCCGGTT	45	0.003821906	20.555555	35
GCGGGTA	45	0.003821906	20.555555	22
ATAGTCC	45	0.003821906	20.555555	3
TTCTGCG	45	0.003821906	20.555555	18
TCTGCGG	55	5.1357754E-4	20.181818	19
TGTACTA	55	5.1357754E-4	20.181818	5
CGGGGTG	75	9.24328E-6	19.733334	17
CTACACT	95	1.6699232E-7	19.473684	4
CCCACGA	115	3.0340743E-9	19.304348	19
GGCGAAT	70	1.2169068E-4	18.5	32
CTAATAC	110	3.8346116E-8	18.5	3
CGGTTAT	50	0.0070277755	18.499998	37
ACCTTAG	50	0.0070277755	18.499998	1
>>END_MODULE