FastQCFastQC Report
Fri 10 Feb 2017
ERR1632830.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632830.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337576
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7310.21654383013010406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.15877906012275755No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3530.10456904519278623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG352.3833494E-531.7142855
CGCCGTC250.00549091329.626
TCCGCCG358.8563684E-426.4285731
TTGTCCG451.320509E-424.66666613
TAGGACC601.3327353E-624.6666664
TACATGC952.7830538E-1023.3684222
CCGGTAC400.001928378123.1253
TCGAACA400.001928378123.12513
ATACTGA502.6966268E-422.26
AACTAGA1151.3460522E-1020.91304427
GGTATCA1700.020.6764721
GACGGAT450.00382029620.55555535
GGGCCTT555.132756E-420.18181833
CCCTTAG555.132756E-420.1818181
GTATCAA4200.019.8214281
TTATAGT759.234014E-619.7333344
ACCCTGC500.00702484318.58
GTCCGCC500.00702484318.530
ATAGTAC1306.91216E-1018.53
TAGTACT500.00702484318.54
Produced by FastQC (version 0.11.5)