##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632830.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337576
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.757805649690738	33.0	33.0	33.0	27.0	33.0
2	31.286492523165155	33.0	33.0	33.0	27.0	33.0
3	31.71407031305543	33.0	33.0	33.0	27.0	33.0
4	31.828557124914095	33.0	33.0	33.0	27.0	33.0
5	31.94590847690594	33.0	33.0	33.0	33.0	33.0
6	34.84406178164325	37.0	37.0	37.0	27.0	37.0
7	34.96786205180463	37.0	37.0	37.0	27.0	37.0
8	35.193429035239475	37.0	37.0	37.0	33.0	37.0
9	35.224977486551175	37.0	37.0	37.0	33.0	37.0
10	35.26821811977155	37.0	37.0	37.0	33.0	37.0
11	35.324495817238194	37.0	37.0	37.0	33.0	37.0
12	35.24535512003223	37.0	37.0	37.0	33.0	37.0
13	35.30210086025073	37.0	37.0	37.0	33.0	37.0
14	35.218934402919636	37.0	37.0	37.0	33.0	37.0
15	35.2727504324952	37.0	37.0	37.0	33.0	37.0
16	35.26976147593431	37.0	37.0	37.0	33.0	37.0
17	35.264144962912056	37.0	37.0	37.0	33.0	37.0
18	35.242529089745716	37.0	37.0	37.0	33.0	37.0
19	35.26766120814276	37.0	37.0	37.0	33.0	37.0
20	35.19958172381923	37.0	37.0	37.0	33.0	37.0
21	35.27459594283954	37.0	37.0	37.0	33.0	37.0
22	35.27061461715288	37.0	37.0	37.0	33.0	37.0
23	35.255015166955	37.0	37.0	37.0	33.0	37.0
24	35.25109308718629	37.0	37.0	37.0	33.0	37.0
25	35.256635542811104	37.0	37.0	37.0	33.0	37.0
26	35.219266180060195	37.0	37.0	37.0	33.0	37.0
27	35.141852501362656	37.0	37.0	37.0	33.0	37.0
28	35.16039054909117	37.0	37.0	37.0	33.0	37.0
29	35.19373118942105	37.0	37.0	37.0	33.0	37.0
30	35.131916960921394	37.0	37.0	37.0	33.0	37.0
31	35.133495864634924	37.0	37.0	37.0	33.0	37.0
32	35.17141621442283	37.0	37.0	37.0	33.0	37.0
33	35.14662179775814	37.0	37.0	37.0	33.0	37.0
34	35.099992298030664	37.0	37.0	37.0	33.0	37.0
35	35.083850747683485	37.0	37.0	37.0	33.0	37.0
36	35.084099580538904	37.0	37.0	37.0	33.0	37.0
37	35.102519136431496	37.0	37.0	37.0	33.0	37.0
38	34.95232184752471	37.0	37.0	37.0	27.0	37.0
39	35.03597708367894	37.0	37.0	37.0	27.0	37.0
40	35.06594663127711	37.0	37.0	37.0	27.0	37.0
41	35.0843750740574	37.0	37.0	37.0	33.0	37.0
42	34.977252529800694	37.0	37.0	37.0	27.0	37.0
43	34.80379233120838	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	5.0
16	10.0
17	7.0
18	9.0
19	9.0
20	17.0
21	64.0
22	154.0
23	411.0
24	808.0
25	1548.0
26	2592.0
27	3918.0
28	5664.0
29	7779.0
30	10024.0
31	13212.0
32	17013.0
33	22343.0
34	31934.0
35	58473.0
36	161581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30025831220229	20.92921297722587	13.342476953337915	25.428051757233927
2	16.208794464061427	22.63727279190464	37.7908974571652	23.363035286868737
3	17.450292674834703	26.98236841481622	30.034718107922366	25.532620802426713
4	12.609604948219067	17.907078702277413	39.416901675474556	30.06641467402896
5	13.548652747825674	37.44016162286418	35.865997582766546	13.145188046543593
6	29.636289309666562	40.00876839585752	17.50776121525227	12.84718107922364
7	26.6268929070787	31.773585799938388	24.394210488897315	17.2053108040856
8	24.300601938526437	36.6225679550679	20.403997914543687	18.67283219186198
9	25.733168234708632	15.39386686257317	20.311278052942153	38.56168684977605
10	14.643517311657227	28.219719411332562	34.71070218261962	22.426061094390594
11	34.00626821811977	23.810934426618005	24.314820958835938	17.867976396426286
12	22.566473919946915	25.996220110434393	30.29628883569922	21.141017133919473
13	27.611263833921846	22.27202170770434	26.2284641088229	23.888250349550916
14	21.710074175889282	20.7864303149513	27.620446951205064	29.883048557954357
15	23.576913050690806	29.244081332796167	23.87047657416404	23.308529042348983
16	23.119534564068537	28.41611962935754	25.122935279759222	23.341410526814702
17	22.368888783562813	27.902457520676826	26.502476479370575	23.22617721638979
18	22.731770031044864	27.780410929686944	27.32273621347489	22.165082825793302
19	24.210251913643148	26.791300329407303	27.148553214683506	21.84989454226604
20	23.521814346991494	26.803445742588334	27.415752304666206	22.258987605753962
21	22.835450387468303	27.299926535061736	27.204836836741947	22.659786240728014
22	22.669265587601014	27.002215797331562	27.239495698746357	23.089022916321063
23	22.642901154110483	27.02769154205275	27.794629949996448	22.534777353840322
24	22.63342180723748	27.88971964831623	26.183140981586366	23.29371756285992
25	22.895288764604118	27.01051022584544	27.563274640377276	22.530926369173162
26	22.889956631988056	27.320958835936203	27.233867336540513	22.555217195535228
27	22.648529516316326	27.614522359409438	26.594900111382323	23.142048012891912
28	22.53951702727682	26.838400834182526	28.00850771381852	22.613574424722135
29	23.74902244235372	27.05198236841482	26.673697182264146	22.52529800696732
30	23.175225726947414	26.935267910041	27.063239092826503	22.826267270185085
31	23.091096523449533	26.890241012394245	27.40212574353627	22.616536720619948
32	22.216923004005025	26.92371495603953	27.488624783752396	23.370737256203046
33	22.392883380335096	27.10648861293457	27.70191008839491	22.798717918335427
34	23.335485935019076	26.26875133303315	27.501658885702774	22.89410384624499
35	22.925207953172027	27.341398677631112	27.12603976586013	22.60735360333673
36	23.66667061639453	27.159809939095197	26.487072540701945	22.686446903808328
37	22.87781121880702	26.530322060810008	27.114783041448444	23.47708367893452
38	22.99185961087281	27.105007464985665	27.048427613337438	22.854705310804086
39	22.513152593786288	26.969926772045405	27.522394956987462	22.994525677180842
40	22.7453965921748	27.126632225039693	27.702798777164254	22.425172405621254
41	22.536554731379006	26.491219754958884	27.444190345285207	23.5280351683769
42	22.394660757873783	27.49306822759912	27.104118776216318	23.00815223831078
43	23.052290447188188	26.31288954191056	27.504917411190373	23.12990259971088
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	8.0
2	12.0
3	49.5
4	87.0
5	87.0
6	112.5
7	138.0
8	131.5
9	125.0
10	162.5
11	200.0
12	200.0
13	305.5
14	411.0
15	728.5
16	1046.0
17	1255.0
18	1464.0
19	1464.0
20	1487.5
21	1511.0
22	1888.0
23	2265.0
24	3072.5
25	3880.0
26	3880.0
27	5198.5
28	6517.0
29	8202.5
30	9888.0
31	11976.5
32	14065.0
33	14065.0
34	16241.5
35	18418.0
36	20383.0
37	22348.0
38	23829.5
39	25311.0
40	25311.0
41	27236.0
42	29161.0
43	29209.5
44	29258.0
45	28857.0
46	28456.0
47	28456.0
48	27133.0
49	25810.0
50	25385.5
51	24961.0
52	23024.0
53	21087.0
54	21087.0
55	20132.5
56	19178.0
57	16605.5
58	14033.0
59	12885.0
60	11737.0
61	11737.0
62	10409.0
63	9081.0
64	7649.0
65	6217.0
66	5187.5
67	4158.0
68	4158.0
69	3398.5
70	2639.0
71	2153.5
72	1668.0
73	1371.0
74	1074.0
75	1074.0
76	838.0
77	602.0
78	462.5
79	323.0
80	253.0
81	183.0
82	183.0
83	155.0
84	127.0
85	99.5
86	72.0
87	58.5
88	45.0
89	45.0
90	28.5
91	12.0
92	7.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	337576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.76282203440266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.20744653034153	57.125631617535966
2	6.35021777744556	8.225160476008135
3	1.8239103495459055	3.5436474412303984
4	0.8333241517288346	2.1587369494153625
5	0.49455304994684673	1.6014325580139346
6	0.35757008477151014	1.389434865893015
7	0.28331604386783793	1.2843842569952664
8	0.21852354629170326	1.1321761231052905
9	0.18156782953494524	1.0582960528209975
>10	1.1154606984679758	14.056620214296265
>50	0.09816294435127682	4.273723558720876
>100	0.035025275918761575	3.772600445717385
>500	9.217177873358308E-4	0.3781554402471461
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	731	0.21654383013010406	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	536	0.15877906012275755	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	353	0.10456904519278623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	2.962295897812641E-4	0.0	0.0
7	0.0	0.0	0.0017773775386875845	0.0	0.0
8	0.0	0.0	0.0023698367182501128	0.0	0.0
9	0.0	0.0	0.003258525487593905	2.962295897812641E-4	0.0
10	8.886887693437922E-4	0.0	0.003258525487593905	2.962295897812641E-4	0.0
11	8.886887693437922E-4	0.0	0.004147214256937697	5.924591795625282E-4	0.0
12	8.886887693437922E-4	0.0	0.0047396734365002255	5.924591795625282E-4	0.0
13	8.886887693437922E-4	0.0	0.005924591795625282	5.924591795625282E-4	0.0
14	8.886887693437922E-4	0.0	0.00651705097518781	8.886887693437922E-4	0.0
15	8.886887693437922E-4	0.0	0.007405739744531602	8.886887693437922E-4	0.0
16	8.886887693437922E-4	0.0	0.007701969334312866	8.886887693437922E-4	0.0
17	8.886887693437922E-4	0.0	0.007998198924094129	0.0011849183591250564	0.0
18	8.886887693437922E-4	0.0	0.007998198924094129	0.0011849183591250564	0.0
19	8.886887693437922E-4	0.0	0.007998198924094129	0.0011849183591250564	0.0
20	8.886887693437922E-4	0.0	0.007998198924094129	0.0011849183591250564	0.0
21	8.886887693437922E-4	0.0	0.008294428513875393	0.0011849183591250564	0.0
22	8.886887693437922E-4	0.0	0.008294428513875393	0.0011849183591250564	0.0
23	8.886887693437922E-4	0.0	0.008294428513875393	0.0014811479489063204	0.0
24	8.886887693437922E-4	0.0	0.008294428513875393	0.0017773775386875845	0.0
25	8.886887693437922E-4	0.0	0.008590658103656657	0.0020736071284688483	0.0
26	8.886887693437922E-4	0.0	0.008590658103656657	0.0020736071284688483	0.0
27	8.886887693437922E-4	0.0	0.008590658103656657	0.003258525487593905	0.0
28	8.886887693437922E-4	0.0	0.008590658103656657	0.009479346873000451	0.0
29	8.886887693437922E-4	0.0	0.008886887693437923	0.023698367182501127	0.0
30	8.886887693437922E-4	0.0	0.008886887693437923	0.04236083133872076	0.0
31	8.886887693437922E-4	0.0	0.008886887693437923	0.10782757068038012	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	35	2.3833494E-5	31.714285	5
CGCCGTC	25	0.005490913	29.6	26
TCCGCCG	35	8.8563684E-4	26.42857	31
TTGTCCG	45	1.320509E-4	24.666666	13
TAGGACC	60	1.3327353E-6	24.666666	4
TACATGC	95	2.7830538E-10	23.368422	2
CCGGTAC	40	0.0019283781	23.125	3
TCGAACA	40	0.0019283781	23.125	13
ATACTGA	50	2.6966268E-4	22.2	6
AACTAGA	115	1.3460522E-10	20.913044	27
GGTATCA	170	0.0	20.676472	1
GACGGAT	45	0.003820296	20.555555	35
GGGCCTT	55	5.132756E-4	20.181818	33
CCCTTAG	55	5.132756E-4	20.181818	1
GTATCAA	420	0.0	19.821428	1
TTATAGT	75	9.234014E-6	19.733334	4
ACCCTGC	50	0.007024843	18.5	8
GTCCGCC	50	0.007024843	18.5	30
ATAGTAC	130	6.91216E-10	18.5	3
TAGTACT	50	0.007024843	18.5	4
>>END_MODULE