FastQCFastQC Report
Fri 10 Feb 2017
ERR1632829.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632829.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287676
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7650.2659241646852709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.19674912053838345No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5110.17763038974401757No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5010.1741542568723147No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4780.16615915126739805No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4430.15399268621643794No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3850.1338311155605612No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC3670.12757407639149598No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3430.11923135749940905No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC3280.11401715819185473No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3110.10810773230995982No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT3090.10741250573561925No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3010.10463159943825692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC250.005489579429.61
CGTGCAA250.005489579429.611
TAACCGT250.005489579429.67
GTGAGTA250.005489579429.62
GTATCAA4300.024.9534871
GGTATCA1600.024.281251
TCGCCAT551.8962364E-523.54545413
ATTGCAG400.001927688623.12522
GCCTTGT502.695283E-422.225
CCGTACA603.7137987E-521.58333217
TACACTG705.08001E-621.1428595
AATTCCG450.00381894320.5555555
TAGAGTC555.1302195E-420.1818185
GTCGCCA555.1302195E-420.18181812
ACAGGGT759.226229E-619.7333343
TAATAGT500.007022378518.54
ATTAGAG500.007022378518.53
AGCCCCG701.2151692E-418.513
ATAGCAC609.2138856E-418.53
ATACTGG701.2151692E-418.56
Produced by FastQC (version 0.11.5)