##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632829.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287676
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.784900374031896	33.0	33.0	33.0	33.0	33.0
2	31.26340744448616	33.0	33.0	33.0	27.0	33.0
3	31.715773995745213	33.0	33.0	33.0	27.0	33.0
4	31.84050807158053	33.0	33.0	33.0	27.0	33.0
5	31.961905059859006	33.0	33.0	33.0	33.0	33.0
6	34.88272918144023	37.0	37.0	37.0	27.0	37.0
7	35.06374184846842	37.0	37.0	37.0	33.0	37.0
8	35.22035206273725	37.0	37.0	37.0	33.0	37.0
9	35.32599869297404	37.0	37.0	37.0	33.0	37.0
10	35.33260682156315	37.0	37.0	37.0	33.0	37.0
11	35.381237920438274	37.0	37.0	37.0	33.0	37.0
12	35.34445000625704	37.0	37.0	37.0	33.0	37.0
13	35.375404969479554	37.0	37.0	37.0	33.0	37.0
14	35.33841891572463	37.0	37.0	37.0	33.0	37.0
15	35.353797327549046	37.0	37.0	37.0	33.0	37.0
16	35.36530332735438	37.0	37.0	37.0	33.0	37.0
17	35.318677957146235	37.0	37.0	37.0	33.0	37.0
18	35.33837024986443	37.0	37.0	37.0	33.0	37.0
19	35.351381415203214	37.0	37.0	37.0	33.0	37.0
20	35.34294136459072	37.0	37.0	37.0	33.0	37.0
21	35.32924192494334	37.0	37.0	37.0	33.0	37.0
22	35.339586896369525	37.0	37.0	37.0	33.0	37.0
23	35.32549812984052	37.0	37.0	37.0	33.0	37.0
24	35.31887609671992	37.0	37.0	37.0	33.0	37.0
25	35.3089899748328	37.0	37.0	37.0	33.0	37.0
26	35.25994174001307	37.0	37.0	37.0	33.0	37.0
27	35.210097470765724	37.0	37.0	37.0	33.0	37.0
28	35.188827013723774	37.0	37.0	37.0	33.0	37.0
29	35.240026974791085	37.0	37.0	37.0	33.0	37.0
30	35.20199808117466	37.0	37.0	37.0	33.0	37.0
31	35.195122290354426	37.0	37.0	37.0	33.0	37.0
32	35.22672381429108	37.0	37.0	37.0	33.0	37.0
33	35.19200072303564	37.0	37.0	37.0	33.0	37.0
34	35.13811023512563	37.0	37.0	37.0	33.0	37.0
35	35.127483696936835	37.0	37.0	37.0	33.0	37.0
36	35.11020731656447	37.0	37.0	37.0	33.0	37.0
37	35.15046788748453	37.0	37.0	37.0	33.0	37.0
38	34.99413923997831	37.0	37.0	37.0	27.0	37.0
39	35.06081146845757	37.0	37.0	37.0	27.0	37.0
40	35.119836204619084	37.0	37.0	37.0	33.0	37.0
41	35.1257560588996	37.0	37.0	37.0	33.0	37.0
42	35.037823801777	37.0	37.0	37.0	27.0	37.0
43	34.83605862150475	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	12.0
17	11.0
18	6.0
19	12.0
20	23.0
21	56.0
22	132.0
23	341.0
24	727.0
25	1295.0
26	2092.0
27	3195.0
28	4652.0
29	6349.0
30	8484.0
31	11003.0
32	13670.0
33	18193.0
34	26326.0
35	48493.0
36	142597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99603720852626	21.276366467831867	13.893407861622103	24.83418846201977
2	15.306803487256495	23.791696213796076	38.53119481639066	22.370305482556766
3	17.59653220984719	26.90040184095997	31.708936442386577	23.794129506806268
4	12.894019661007523	18.28306845200851	39.18157927668627	29.641332610297695
5	13.282998929351075	37.90861941906867	36.003003378801154	12.8053782727791
6	28.966615219900167	39.760007786537635	18.022358486630793	13.251018506931409
7	25.06882743085972	33.20158789749579	24.24046496753292	17.48911970411157
8	23.474672895896774	36.85778445195289	21.32920368748175	18.338338964668583
9	25.30381401298683	16.04513410920619	20.373267147763457	38.27778473004352
10	14.141256135374519	28.808451174237682	34.9904058732741	22.059886817113696
11	32.60265020370139	24.077781949137222	25.220386824065965	18.099181023095426
12	21.56766640248057	26.36924873816377	30.9598298085346	21.103255050821062
13	26.811065226157204	22.515607836593947	27.596671255162057	23.07665568208679
14	21.499186584908024	21.712273529943406	27.929684784271192	28.858855100877378
15	23.285571267676136	29.372279926027893	24.86999263059831	22.47215617569766
16	22.634491580806184	29.460921314256318	25.192577761092338	22.71200934384516
17	21.98063098763887	28.579721631279636	26.613273265757307	22.82637411532418
18	21.95664567082412	28.281469430887523	28.061082606821564	21.70080229146679
19	23.83167174182066	27.389841349295736	28.085415536923485	20.693071371960123
20	23.691583587091035	26.798203534531908	28.17961873774663	21.33059414063043
21	22.04911080521142	28.154590581070373	27.22368219802834	22.572616415689872
22	21.744601565650246	27.94845590177839	27.400964974485188	22.905977558086178
23	21.876694614774955	28.135819463563177	27.612313853084718	22.375172068577147
24	22.921620156008842	28.15320012792169	26.953934287184193	21.971245428885275
25	23.029032661744463	27.18440189657809	27.75622575397322	22.030339687704224
26	22.682114601148516	27.400617361198016	27.2042158539468	22.71305218370667
27	22.395333639233026	27.46701149904754	27.247667514843087	22.889987346876346
28	21.62780350116103	27.929684784271192	28.056216020801177	22.3862956937666
29	22.84340716639553	28.0325783172736	27.112098332846674	22.0119161834842
30	22.150266271777973	28.211946773453466	27.40583156050557	22.23195539426299
31	22.793003239755834	27.62726122443304	27.534100863471405	22.045634672339716
32	21.77310585519821	27.55565288727596	27.84173862261711	22.829502634908717
33	22.101600411574132	27.414869505971996	27.900485268148888	22.58304481430498
34	22.01747799607892	27.615789985956425	27.62691361114587	22.73981840681878
35	22.30008759854837	28.08020133761593	27.347432528260963	22.272278535574745
36	22.644224752846952	27.849733728222027	27.119050598590082	22.38699092034094
37	23.006090184791223	26.86181676608407	27.29841905476995	22.83367399435476
38	23.039113447072403	27.554957660701625	27.066908605514538	22.33902028671144
39	22.218746089350518	27.493777722159653	27.855295540816755	22.43218064767308
40	22.313992130035178	27.54418164879934	27.836176810022383	22.30564941114309
41	22.05258693808312	27.436421529776556	27.95610339409614	22.554888138044188
42	22.516998289742627	27.31267119954393	27.570947871911457	22.599382638801984
43	22.278883188030978	26.8778069772939	27.91543263949721	22.92787719517791
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	12.5
2	23.0
3	62.5
4	102.0
5	102.0
6	123.5
7	145.0
8	141.5
9	138.0
10	190.5
11	243.0
12	243.0
13	370.5
14	498.0
15	915.5
16	1333.0
17	1558.5
18	1784.0
19	1784.0
20	1876.5
21	1969.0
22	2554.0
23	3139.0
24	3909.0
25	4679.0
26	4679.0
27	5787.0
28	6895.0
29	9012.5
30	11130.0
31	12132.5
32	13135.0
33	13135.0
34	14542.5
35	15950.0
36	17193.0
37	18436.0
38	20084.0
39	21732.0
40	21732.0
41	22998.5
42	24265.0
43	23082.5
44	21900.0
45	21983.0
46	22066.0
47	22066.0
48	21670.5
49	21275.0
50	21593.5
51	21912.0
52	21585.5
53	21259.0
54	21259.0
55	18219.0
56	15179.0
57	13328.0
58	11477.0
59	10048.0
60	8619.0
61	8619.0
62	7462.5
63	6306.0
64	5230.5
65	4155.0
66	3570.0
67	2985.0
68	2985.0
69	2423.0
70	1861.0
71	1563.5
72	1266.0
73	1043.0
74	820.0
75	820.0
76	629.5
77	439.0
78	329.5
79	220.0
80	179.0
81	138.0
82	138.0
83	106.0
84	74.0
85	73.0
86	72.0
87	55.0
88	38.0
89	38.0
90	26.5
91	15.0
92	8.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	287676.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.55400011750767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.57135270191591	56.03046407440969
2	5.507116785693131	6.889843681187531
3	1.5298998035591163	2.871040574748358
4	0.7766123542421678	1.943208371940899
5	0.4705739202175484	1.4718140530292285
6	0.3165925800378983	1.1882479373335648
7	0.2247597398377312	0.9841734554554303
8	0.18403399247604554	0.9209649909577566
9	0.14212344538332616	0.8001351017288262
>10	1.0745754044312419	13.678514526899813
>50	0.13416696951730087	5.7749966033052145
>100	0.06597466682875539	6.634032550121634
>500	0.002217635859790097	0.8125640788820632
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	765	0.2659241646852709	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	566	0.19674912053838345	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	511	0.17763038974401757	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	501	0.1741542568723147	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	478	0.16615915126739805	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	443	0.15399268621643794	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	385	0.1338311155605612	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	367	0.12757407639149598	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	343	0.11923135749940905	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	328	0.11401715819185473	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	311	0.10810773230995982	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	309	0.10741250573561925	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	301	0.10463159943825692	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	6.952265743405776E-4	0.0	0.0
4	0.0	0.0	6.952265743405776E-4	0.0	0.0
5	0.0	3.476132871702888E-4	0.0010428398615108664	0.0	0.0
6	0.0	3.476132871702888E-4	0.001390453148681155	0.0	0.0
7	0.0	3.476132871702888E-4	0.004866586020384043	0.0	0.0
8	0.0	3.476132871702888E-4	0.00590942588189491	0.0	0.0
9	0.0	3.476132871702888E-4	0.006952265743405776	6.952265743405776E-4	0.0
10	0.0	3.476132871702888E-4	0.008342718892086931	6.952265743405776E-4	0.0
11	0.0	3.476132871702888E-4	0.010776011902278953	6.952265743405776E-4	0.0
12	0.0	3.476132871702888E-4	0.010776011902278953	6.952265743405776E-4	0.0
13	0.0	3.476132871702888E-4	0.01147123847661953	6.952265743405776E-4	0.0
14	0.0	3.476132871702888E-4	0.01181885176378982	6.952265743405776E-4	0.0
15	0.0	3.476132871702888E-4	0.012861691625300686	6.952265743405776E-4	0.0
16	0.0	3.476132871702888E-4	0.013904531486811552	6.952265743405776E-4	0.0
17	0.0	3.476132871702888E-4	0.013904531486811552	6.952265743405776E-4	0.0
18	0.0	3.476132871702888E-4	0.01425214477398184	6.952265743405776E-4	0.0
19	0.0	3.476132871702888E-4	0.01425214477398184	6.952265743405776E-4	0.0
20	0.0	3.476132871702888E-4	0.01425214477398184	6.952265743405776E-4	0.0
21	0.0	3.476132871702888E-4	0.015294984635492706	6.952265743405776E-4	0.0
22	0.0	3.476132871702888E-4	0.015990211209833283	0.0010428398615108664	0.0
23	0.0	3.476132871702888E-4	0.015990211209833283	0.001390453148681155	0.0
24	0.0	3.476132871702888E-4	0.015990211209833283	0.0020856797230217328	0.0
25	0.0	3.476132871702888E-4	0.015990211209833283	0.0020856797230217328	0.0
26	0.0	3.476132871702888E-4	0.016337824497003575	0.00278090629736231	0.0
27	0.0	3.476132871702888E-4	0.016337824497003575	0.003476132871702888	0.0
28	0.0	3.476132871702888E-4	0.016337824497003575	0.009733172040768087	0.0
29	0.0	3.476132871702888E-4	0.016337824497003575	0.02294247695323906	0.0
30	0.0	3.476132871702888E-4	0.016337824497003575	0.04692779376798899	0.0
31	0.0	3.476132871702888E-4	0.016337824497003575	0.10671727916127866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAC	25	0.0054895794	29.6	1
CGTGCAA	25	0.0054895794	29.6	11
TAACCGT	25	0.0054895794	29.6	7
GTGAGTA	25	0.0054895794	29.6	2
GTATCAA	430	0.0	24.953487	1
GGTATCA	160	0.0	24.28125	1
TCGCCAT	55	1.8962364E-5	23.545454	13
ATTGCAG	40	0.0019276886	23.125	22
GCCTTGT	50	2.695283E-4	22.2	25
CCGTACA	60	3.7137987E-5	21.583332	17
TACACTG	70	5.08001E-6	21.142859	5
AATTCCG	45	0.003818943	20.555555	5
TAGAGTC	55	5.1302195E-4	20.181818	5
GTCGCCA	55	5.1302195E-4	20.181818	12
ACAGGGT	75	9.226229E-6	19.733334	3
TAATAGT	50	0.0070223785	18.5	4
ATTAGAG	50	0.0070223785	18.5	3
AGCCCCG	70	1.2151692E-4	18.5	13
ATAGCAC	60	9.2138856E-4	18.5	3
ATACTGG	70	1.2151692E-4	18.5	6
>>END_MODULE