FastQCFastQC Report
Fri 10 Feb 2017
ERR1632828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287283
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6080.21163800155247614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.16847498807795797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA250.005489566429.623
GGTATCA1550.025.0645161
TAACCAG400.001927681323.1250025
GGGGTTA400.001927681323.1250026
GGGTTAG400.001927681323.1250027
ATATAGC603.7137746E-521.5833346
ATAGGGG450.003818930120.5555553
TAACGTC450.003818930120.55555527
TCTCGAT450.003818930120.55555523
CTCCTAG450.003818930120.5555551
AACTAGA555.130197E-420.18181827
ACTGTGT656.879121E-519.9230778
CATAATA656.879121E-519.9230772
CTTAACC500.007022354818.53
GTCGCCC609.21384E-418.537
GTATTAT609.21384E-418.51
TTAGTCC500.007022354818.510
CTATTAT500.007022354818.51
TATACTG500.007022354818.55
CTCTAAT609.21384E-418.51
Produced by FastQC (version 0.11.5)