##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632828.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287283
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6696010554053	33.0	33.0	33.0	27.0	33.0
2	31.189910297511513	33.0	33.0	33.0	27.0	33.0
3	31.635101972619335	33.0	33.0	33.0	27.0	33.0
4	31.78101036260412	33.0	33.0	33.0	27.0	33.0
5	31.88722618463327	33.0	33.0	33.0	33.0	33.0
6	34.761197147064046	37.0	37.0	37.0	27.0	37.0
7	34.93966924600481	37.0	37.0	37.0	27.0	37.0
8	35.103103907993166	37.0	37.0	37.0	33.0	37.0
9	35.190206173007105	37.0	37.0	37.0	33.0	37.0
10	35.23325779805975	37.0	37.0	37.0	33.0	37.0
11	35.252503628825934	37.0	37.0	37.0	33.0	37.0
12	35.216932432479474	37.0	37.0	37.0	33.0	37.0
13	35.23510266879697	37.0	37.0	37.0	33.0	37.0
14	35.21431480456553	37.0	37.0	37.0	33.0	37.0
15	35.23071674968585	37.0	37.0	37.0	33.0	37.0
16	35.22562072938531	37.0	37.0	37.0	33.0	37.0
17	35.173379559528406	37.0	37.0	37.0	33.0	37.0
18	35.20048871670095	37.0	37.0	37.0	33.0	37.0
19	35.199503625345045	37.0	37.0	37.0	33.0	37.0
20	35.21588816602444	37.0	37.0	37.0	33.0	37.0
21	35.20019632209355	37.0	37.0	37.0	33.0	37.0
22	35.204181939063574	37.0	37.0	37.0	33.0	37.0
23	35.21027349338457	37.0	37.0	37.0	33.0	37.0
24	35.203544936526	37.0	37.0	37.0	33.0	37.0
25	35.2039870093253	37.0	37.0	37.0	33.0	37.0
26	35.17451084818802	37.0	37.0	37.0	33.0	37.0
27	35.11350828277343	37.0	37.0	37.0	33.0	37.0
28	35.0584162654943	37.0	37.0	37.0	33.0	37.0
29	35.118270137808366	37.0	37.0	37.0	33.0	37.0
30	35.07592513305695	37.0	37.0	37.0	33.0	37.0
31	35.053208856771896	37.0	37.0	37.0	27.0	37.0
32	35.10606962472544	37.0	37.0	37.0	33.0	37.0
33	35.074776439956416	37.0	37.0	37.0	33.0	37.0
34	34.994656836638434	37.0	37.0	37.0	27.0	37.0
35	35.01798923013196	37.0	37.0	37.0	27.0	37.0
36	34.97304748279571	37.0	37.0	37.0	27.0	37.0
37	35.025594970812755	37.0	37.0	37.0	27.0	37.0
38	34.890557394624814	37.0	37.0	37.0	27.0	37.0
39	34.95537501348844	37.0	37.0	37.0	27.0	37.0
40	35.01132332926069	37.0	37.0	37.0	27.0	37.0
41	35.017637660425436	37.0	37.0	37.0	27.0	37.0
42	34.91964021539736	37.0	37.0	37.0	27.0	37.0
43	34.73113619671195	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	12.0
17	15.0
18	10.0
19	8.0
20	28.0
21	51.0
22	180.0
23	434.0
24	860.0
25	1537.0
26	2469.0
27	3697.0
28	5131.0
29	6935.0
30	8933.0
31	11410.0
32	14366.0
33	18868.0
34	26953.0
35	48277.0
36	137104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.20257724961101	20.117793256127232	13.115986675160036	25.563642819101723
2	16.365047705572554	22.512644326326306	36.89845901080119	24.223848957299946
3	18.31225655538267	25.731421629542993	29.753936014313414	26.202385800760926
4	13.167851909093123	17.486241789454997	38.06107566406644	31.284830637385436
5	14.641311877138572	36.470309764239445	35.020867924659655	13.86751043396233
6	31.001834428072666	38.77744245221611	17.07445271735536	13.146270402355864
7	27.45794216852372	31.317899075128008	23.271477950313802	17.95268080603447
8	25.12818370735477	35.576417678734906	20.47423620611035	18.821162407799974
9	25.874138045063578	15.182241900843419	19.67258765746668	39.27103239662632
10	14.492677951706156	28.346612921753117	33.93657125552156	23.224137871019167
11	34.74309304762203	23.045220218390924	23.974617363366438	18.237069370620606
12	22.94218592816143	24.94300045599635	30.646783833362925	21.4680297824793
13	28.50255671237073	21.61527135263834	25.7996470379382	24.082524897052732
14	22.232084738741936	20.167221868331925	26.875937664254412	30.724755728671727
15	23.891424135782486	28.7977360303255	23.534633096981025	23.77620673691099
16	23.923448307070032	27.852326799706212	24.49431396915237	23.729910924071387
17	22.96515979017206	27.51189593536687	26.021727704040963	23.50121657042011
18	23.560739758356743	26.996724484219392	26.876285753072754	22.56625000435111
19	24.775221645555078	26.470066102066603	26.195424024394065	22.559288227984254
20	24.19948274001594	26.502786450990833	26.647939488239818	22.649791320753405
21	23.609472192924745	26.534114444641695	26.86131793388401	22.995095428549547
22	23.301413588691293	26.37294932174894	26.840432604783437	23.485204484776332
23	23.30628683214809	26.278617251978016	26.91422743427213	23.500868481601767
24	23.34005144752735	26.79170017021543	26.057928941148624	23.810319441108593
25	23.68605173296018	26.472850812613345	26.833470828416576	23.00762662600989
26	23.77620673691099	26.510096316176035	26.51810235899792	23.195594587915053
27	23.559695491901714	26.71546871899834	26.240327481960296	23.484508307139652
28	23.31324860851495	26.47702787843346	26.746448623830855	23.463274889220735
29	23.908480487881288	26.359025769015222	26.39035376266608	23.34213998043741
30	23.865665563225114	26.343709861008136	26.363550923653676	23.427073652113073
31	23.692317331690354	26.230580995046697	26.586327767393126	23.490773905869823
32	23.290970924141003	26.284882850708186	26.878374285982808	23.545771939168
33	23.339355269890667	26.29010418298333	27.023875412050142	23.346665135075867
34	23.684659377686813	26.147387767462742	26.52123515836301	23.646717696487435
35	23.364417664811356	26.74087920273737	26.494084230532263	23.400618901919014
36	23.950251146082437	26.39000567384774	26.34440603864482	23.315337141425005
37	23.72294914770453	26.021379615222624	26.498261296352375	23.757409940720475
38	23.687792177051897	26.483293477163635	26.48259729952695	23.34631704625752
39	23.30628683214809	26.453009749967805	26.634364024324448	23.60633939355966
40	23.306983009784776	26.83973642714675	26.6120863399505	23.24119422311797
41	23.507134080331937	25.977520424111418	26.903784769721845	23.611560725834803
42	23.278091637862317	26.550822707922155	26.57727745811621	23.593808196099317
43	23.643236808304007	25.76623051137728	26.831730384324864	23.758802295993846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.5
2	6.0
3	36.5
4	67.0
5	67.0
6	84.5
7	102.0
8	93.0
9	84.0
10	114.0
11	144.0
12	144.0
13	230.5
14	317.0
15	592.5
16	868.0
17	1073.5
18	1279.0
19	1279.0
20	1283.0
21	1287.0
22	1619.5
23	1952.0
24	2640.0
25	3328.0
26	3328.0
27	4380.5
28	5433.0
29	6663.5
30	7894.0
31	9269.0
32	10644.0
33	10644.0
34	12249.5
35	13855.0
36	15410.5
37	16966.0
38	18109.0
39	19252.0
40	19252.0
41	20125.5
42	20999.0
43	21732.0
44	22465.0
45	22533.5
46	22602.0
47	22602.0
48	22316.5
49	22031.0
50	21789.5
51	21548.0
52	20533.5
53	19519.0
54	19519.0
55	18706.0
56	17893.0
57	16108.0
58	14323.0
59	13563.0
60	12803.0
61	12803.0
62	11553.5
63	10304.0
64	8661.5
65	7019.0
66	5862.5
67	4706.0
68	4706.0
69	3869.5
70	3033.0
71	2455.5
72	1878.0
73	1518.5
74	1159.0
75	1159.0
76	889.5
77	620.0
78	502.5
79	385.0
80	315.0
81	245.0
82	245.0
83	189.5
84	134.0
85	108.0
86	82.0
87	57.0
88	32.0
89	32.0
90	26.0
91	20.0
92	11.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	287283.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.51385423417653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95505375351117	64.6591500079478
2	6.910647559817411	10.160566747523697
3	2.002054135466435	4.415361476508295
4	0.9694983569526621	2.850862435731666
5	0.5388700388564477	1.9807206743828965
6	0.36148284263553454	1.5944398200998675
7	0.24028609155895106	1.2365049696555306
8	0.1738333338591374	1.0223326693089256
9	0.13086611537412055	0.8658425276825423
>10	0.6821407075397375	8.750881375425228
>50	0.027641753200620383	1.4066703516385248
>100	0.007148729276022419	0.8436518572218018
>500	4.7658195173482795E-4	0.21301508687327883
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.21163800155247614	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	484	0.16847498807795797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	6.961776366857768E-4	0.0	0.0
8	0.0	0.0	0.0010442664550286652	0.0	0.0
9	0.0	0.0	0.0013923552733715535	0.0	0.0
10	0.0	0.0	0.0013923552733715535	0.0	0.0
11	0.0	0.0	0.002784710546743107	0.0	0.0
12	0.0	0.0	0.0034808881834288838	0.0	0.0
13	0.0	0.0	0.0038289770017717723	0.0	0.0
14	0.0	0.0	0.004525154638457549	0.0	0.0
15	3.480888183428884E-4	0.0	0.004873243456800437	0.0	0.0
16	3.480888183428884E-4	0.0	0.004873243456800437	0.0	0.0
17	3.480888183428884E-4	0.0	0.005221332275143325	0.0	0.0
18	3.480888183428884E-4	0.0	0.005221332275143325	0.0	0.0
19	3.480888183428884E-4	0.0	0.005221332275143325	0.0	0.0
20	3.480888183428884E-4	0.0	0.005569421093486214	0.0	0.0
21	3.480888183428884E-4	0.0	0.005917509911829102	0.0	0.0
22	3.480888183428884E-4	0.0	0.005917509911829102	3.480888183428884E-4	0.0
23	3.480888183428884E-4	0.0	0.005917509911829102	3.480888183428884E-4	0.0
24	3.480888183428884E-4	0.0	0.005917509911829102	6.961776366857768E-4	0.0
25	3.480888183428884E-4	0.0	0.005917509911829102	6.961776366857768E-4	0.0
26	3.480888183428884E-4	0.0	0.005917509911829102	6.961776366857768E-4	0.0
27	3.480888183428884E-4	0.0	0.005917509911829102	0.0013923552733715535	0.0
28	3.480888183428884E-4	0.0	0.005917509911829102	0.006613687548514879	0.0
29	3.480888183428884E-4	0.0	0.005917509911829102	0.018796796190515972	0.0
30	3.480888183428884E-4	0.0	0.005917509911829102	0.038289770017717724	0.0
31	3.480888183428884E-4	0.0	0.005917509911829102	0.0898069151324652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAA	25	0.0054895664	29.6	23
GGTATCA	155	0.0	25.064516	1
TAACCAG	40	0.0019276813	23.125002	5
GGGGTTA	40	0.0019276813	23.125002	6
GGGTTAG	40	0.0019276813	23.125002	7
ATATAGC	60	3.7137746E-5	21.583334	6
ATAGGGG	45	0.0038189301	20.555555	3
TAACGTC	45	0.0038189301	20.555555	27
TCTCGAT	45	0.0038189301	20.555555	23
CTCCTAG	45	0.0038189301	20.555555	1
AACTAGA	55	5.130197E-4	20.181818	27
ACTGTGT	65	6.879121E-5	19.923077	8
CATAATA	65	6.879121E-5	19.923077	2
CTTAACC	50	0.0070223548	18.5	3
GTCGCCC	60	9.21384E-4	18.5	37
GTATTAT	60	9.21384E-4	18.5	1
TTAGTCC	50	0.0070223548	18.5	10
CTATTAT	50	0.0070223548	18.5	1
TATACTG	50	0.0070223548	18.5	5
CTCTAAT	60	9.21384E-4	18.5	1
>>END_MODULE