FastQCFastQC Report
Fri 10 Feb 2017
ERR1632827.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632827.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294481
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6310.21427528431375878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.15586744136294023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGT303.5930355E-430.83333423
CTAAGCG250.005489787629.630
CGTACTG358.853669E-426.4285725
GGTATCA1150.025.739131
AGCCGGT451.3199491E-424.66666831
GCCGGTG400.001927795723.12500232
CGGAGTT400.001927795723.12500224
GTATCAA3400.021.7647061
AGCGTTC603.714182E-521.58333437
ATAAGAC603.714182E-521.5833343
CGTATTA450.003819153620.5555572
AAGCGTA450.003819153620.55555732
TAGCCGG450.003819153620.55555730
CCGTATT450.003819153620.5555571
GAGTTAG450.003819153620.55555726
TATTATA951.6655576E-719.4736842
CATATAA609.21459E-418.52
TTAGCCG500.007022763618.529
GCACGGA500.007022763618.521
GTTAGCC500.007022763618.528
Produced by FastQC (version 0.11.5)