##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294481
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.810133760751967	33.0	33.0	33.0	33.0	33.0
2	31.321569133492485	33.0	33.0	33.0	27.0	33.0
3	31.75147802404909	33.0	33.0	33.0	27.0	33.0
4	31.86528163107297	33.0	33.0	33.0	33.0	33.0
5	31.969444548205146	33.0	33.0	33.0	33.0	33.0
6	34.905379294419674	37.0	37.0	37.0	27.0	37.0
7	35.025169705346016	37.0	37.0	37.0	33.0	37.0
8	35.224065389617664	37.0	37.0	37.0	33.0	37.0
9	35.27737612952958	37.0	37.0	37.0	33.0	37.0
10	35.33210631585739	37.0	37.0	37.0	33.0	37.0
11	35.3586207599132	37.0	37.0	37.0	33.0	37.0
12	35.31039353982091	37.0	37.0	37.0	33.0	37.0
13	35.35632180004822	37.0	37.0	37.0	33.0	37.0
14	35.30871261643366	37.0	37.0	37.0	33.0	37.0
15	35.343373596259184	37.0	37.0	37.0	33.0	37.0
16	35.33435433865003	37.0	37.0	37.0	33.0	37.0
17	35.307598792451806	37.0	37.0	37.0	33.0	37.0
18	35.30184969488694	37.0	37.0	37.0	33.0	37.0
19	35.349553961036534	37.0	37.0	37.0	33.0	37.0
20	35.261500741983355	37.0	37.0	37.0	33.0	37.0
21	35.326982046379904	37.0	37.0	37.0	33.0	37.0
22	35.34170625609122	37.0	37.0	37.0	33.0	37.0
23	35.322835768691355	37.0	37.0	37.0	33.0	37.0
24	35.2908438914565	37.0	37.0	37.0	33.0	37.0
25	35.31317130816589	37.0	37.0	37.0	33.0	37.0
26	35.271521762015205	37.0	37.0	37.0	33.0	37.0
27	35.210145985649326	37.0	37.0	37.0	33.0	37.0
28	35.208828413378114	37.0	37.0	37.0	33.0	37.0
29	35.253490038406554	37.0	37.0	37.0	33.0	37.0
30	35.20319816898204	37.0	37.0	37.0	33.0	37.0
31	35.185974646921196	37.0	37.0	37.0	33.0	37.0
32	35.23365174663221	37.0	37.0	37.0	33.0	37.0
33	35.199425429824	37.0	37.0	37.0	33.0	37.0
34	35.14262719835915	37.0	37.0	37.0	33.0	37.0
35	35.153938624223635	37.0	37.0	37.0	33.0	37.0
36	35.1472387013084	37.0	37.0	37.0	33.0	37.0
37	35.15951113993772	37.0	37.0	37.0	33.0	37.0
38	35.0205785772257	37.0	37.0	37.0	27.0	37.0
39	35.07508124463038	37.0	37.0	37.0	27.0	37.0
40	35.11247245153338	37.0	37.0	37.0	33.0	37.0
41	35.15462797260265	37.0	37.0	37.0	33.0	37.0
42	35.02369932185778	37.0	37.0	37.0	27.0	37.0
43	34.85701624213447	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	8.0
17	16.0
18	9.0
19	6.0
20	20.0
21	50.0
22	126.0
23	344.0
24	726.0
25	1337.0
26	2186.0
27	3274.0
28	4777.0
29	6513.0
30	8532.0
31	10888.0
32	14254.0
33	18758.0
34	26981.0
35	50788.0
36	144879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.220931061766294	20.949059531854346	13.400524991425595	25.42948441495377
2	15.912741399275335	22.98756116693437	37.57084497811404	23.528852455676258
3	17.545444358040076	26.72905892060948	30.222662922225886	25.502833799124563
4	12.728155636526637	17.95735548303626	38.96550201880597	30.34898686163114
5	13.914310261103433	37.1779503601251	35.55102026955899	13.356719109212477
6	30.16119885493461	39.37537566090851	17.52574869006829	12.937676794088581
7	26.78169389536167	31.88083441716104	23.969627921665573	17.367843765811717
8	24.381878627144026	36.35854265640228	20.774175583484165	18.48540313296953
9	25.487892257904583	15.536825805400008	20.32762724929622	38.64765468739918
10	14.48005134456892	28.3607431379274	34.35162200617357	22.807583511330105
11	33.967556480723715	23.491159022144043	24.57102495576964	17.9702595413626
12	22.496188209086494	25.501815057677746	30.867186677578516	21.13481005565724
13	27.603478662460397	22.0710334452817	26.461809081061254	23.86367881119665
14	21.644859940030088	20.71033445281699	27.554579073013198	30.090226534139724
15	23.60050393743569	28.851436934810735	23.97370288745284	23.57435624030073
16	22.968544659927126	28.487406657814933	25.211134164852737	23.332914517405197
17	22.443892814816575	27.85408905837728	26.61869526387101	23.08332286293513
18	22.89213905141588	27.92574054013672	27.499567034885104	21.682553373562303
19	23.948234351282427	26.936203014795524	26.987140087136353	22.1284225467857
20	23.704075984528714	26.835008031078406	27.081203880725752	22.379712103667128
21	23.06328761448107	27.345737076415794	27.055056183590793	22.53591912551234
22	22.765135951046076	27.05233953973261	27.07746849542076	23.10505601380055
23	22.851049813060946	26.995290018710882	27.463911084246522	22.68974908398165
24	22.98314662066483	27.469683952445152	26.25262750398158	23.294541922908437
25	23.24190694815625	26.918205249235093	27.172890610939245	22.66699719166941
26	22.919305489997658	27.48836087897012	26.972877706880922	22.619455924151303
27	23.072116707020147	27.219752717492806	26.63193890267963	23.076191672807415
28	22.89349737334497	26.92635518080963	27.29785622841542	22.882291217429987
29	23.65755345845742	26.88934090824196	26.751471232439446	22.701634400861177
30	23.24258610912079	27.11346402654161	26.8570807624261	22.786869101911496
31	23.34412067332018	26.814972782624345	27.0475854129808	22.79332113107467
32	22.573612559044555	26.84383712361748	27.32196644265674	23.260583874681217
33	22.55663353493095	27.00004414546269	27.54710830240321	22.89621401720315
34	23.06770216075061	26.694761291899987	27.2968374869686	22.940699060380805
35	22.91183471938767	27.23469425871279	27.275783497067724	22.57768752483182
36	23.71290507706779	26.81768942648252	26.66250114608413	22.80690435036556
37	22.88263079791226	26.648917926793242	26.96880274109365	23.499648534200848
38	23.08060621907695	26.936542595277796	26.998346243051337	22.98450494259392
39	22.71487803966979	27.037737578994907	27.00343995028542	23.243944431049883
40	22.7685317558688	27.26593566308183	27.35388700799033	22.611645573059043
41	22.734913288123852	26.512066992437543	27.433688421324298	23.31933129811431
42	22.671072157456678	27.29547916503951	27.00581701366132	23.027631663842488
43	23.10369769187146	26.35144542432279	27.29887496986223	23.245981913943513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	44.0
4	79.0
5	79.0
6	96.5
7	114.0
8	115.0
9	116.0
10	151.0
11	186.0
12	186.0
13	283.0
14	380.0
15	674.5
16	969.0
17	1164.0
18	1359.0
19	1359.0
20	1464.0
21	1569.0
22	1908.0
23	2247.0
24	3061.5
25	3876.0
26	3876.0
27	5011.5
28	6147.0
29	7560.0
30	8973.0
31	10642.5
32	12312.0
33	12312.0
34	13995.0
35	15678.0
36	17331.0
37	18984.0
38	20604.0
39	22224.0
40	22224.0
41	23259.0
42	24294.0
43	24049.0
44	23804.0
45	23548.5
46	23293.0
47	23293.0
48	22408.5
49	21524.0
50	21333.5
51	21143.0
52	19937.5
53	18732.0
54	18732.0
55	17778.5
56	16825.0
57	15024.5
58	13224.0
59	12246.5
60	11269.0
61	11269.0
62	9970.0
63	8671.0
64	7184.5
65	5698.0
66	4907.0
67	4116.0
68	4116.0
69	3435.5
70	2755.0
71	2174.0
72	1593.0
73	1293.5
74	994.0
75	994.0
76	768.0
77	542.0
78	451.0
79	360.0
80	278.5
81	197.0
82	197.0
83	153.5
84	110.0
85	92.5
86	75.0
87	48.0
88	21.0
89	21.0
90	17.5
91	14.0
92	7.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	294481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.56977459924629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.31734046518788	64.81739265362823
2	6.046929279699179	8.776485896907042
3	1.7325419985152153	3.771905469479306
4	0.8152871362421771	2.3666081484303914
5	0.4658671298668027	1.6903936303815834
6	0.3179111428916409	1.3842443989341096
7	0.22357828242315458	1.1357517892514468
8	0.1798805197948717	1.044311102104728
9	0.11605448467151505	0.7579843014459178
>10	0.7215119350172149	9.720023365274178
>50	0.042379002329376914	2.047556044836502
>100	0.020247745557368454	2.271721059090921
>500	4.708778036597315E-4	0.21562214023561538
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	631	0.21427528431375878	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	459	0.15586744136294023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	6.791609645444019E-4	0.0	0.0
2	0.0	0.0	6.791609645444019E-4	0.0	0.0
3	0.0	0.0	0.0010187414468166027	0.0	0.0
4	0.0	0.0	0.0010187414468166027	0.0	0.0
5	0.0	0.0	0.0010187414468166027	0.0	0.0
6	0.0	0.0	0.0020374828936332054	0.0	0.0
7	0.0	0.0	0.0023770633759054066	0.0	0.0
8	0.0	0.0	0.0030562243404498083	0.0	0.0
9	0.0	0.0	0.003395804822722009	6.791609645444019E-4	0.0
10	0.0	0.0	0.0037353853049942104	6.791609645444019E-4	0.0
11	0.0	0.0	0.005093707234083014	0.0010187414468166027	0.0
12	0.0	0.0	0.005093707234083014	0.0010187414468166027	0.0
13	0.0	0.0	0.0061124486808996166	0.0010187414468166027	0.0
14	0.0	0.0	0.0061124486808996166	0.0010187414468166027	0.0
15	0.0	0.0	0.0071311901277162195	0.0010187414468166027	0.0
16	0.0	0.0	0.007470770609988421	0.0010187414468166027	0.0
17	0.0	0.0	0.007810351092260621	0.0010187414468166027	0.0
18	0.0	0.0	0.008149931574532822	0.0010187414468166027	0.0
19	0.0	0.0	0.008149931574532822	0.0010187414468166027	0.0
20	0.0	0.0	0.008489512056805024	0.0010187414468166027	0.0
21	0.0	0.0	0.008829092539077224	0.0010187414468166027	0.0
22	0.0	0.0	0.008829092539077224	0.0010187414468166027	0.0
23	0.0	0.0	0.008829092539077224	0.0013583219290888037	0.0
24	0.0	0.0	0.008829092539077224	0.0016979024113610046	0.0
25	0.0	0.0	0.009168673021349424	0.0020374828936332054	0.0
26	0.0	0.0	0.009168673021349424	0.0023770633759054066	0.0
27	0.0	0.0	0.009168673021349424	0.0027166438581776075	0.0
28	0.0	0.0	0.009168673021349424	0.008489512056805024	0.0
29	0.0	0.0	0.009168673021349424	0.019016507007243253	0.0
30	0.0	0.0	0.009168673021349424	0.0370142725676699	0.0
31	0.0	0.0	0.009168673021349424	0.08489512056805024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGAGT	30	3.5930355E-4	30.833334	23
CTAAGCG	25	0.0054897876	29.6	30
CGTACTG	35	8.853669E-4	26.428572	5
GGTATCA	115	0.0	25.73913	1
AGCCGGT	45	1.3199491E-4	24.666668	31
GCCGGTG	40	0.0019277957	23.125002	32
CGGAGTT	40	0.0019277957	23.125002	24
GTATCAA	340	0.0	21.764706	1
AGCGTTC	60	3.714182E-5	21.583334	37
ATAAGAC	60	3.714182E-5	21.583334	3
CGTATTA	45	0.0038191536	20.555557	2
AAGCGTA	45	0.0038191536	20.555557	32
TAGCCGG	45	0.0038191536	20.555557	30
CCGTATT	45	0.0038191536	20.555557	1
GAGTTAG	45	0.0038191536	20.555557	26
TATTATA	95	1.6655576E-7	19.473684	2
CATATAA	60	9.21459E-4	18.5	2
TTAGCCG	50	0.0070227636	18.5	29
GCACGGA	50	0.0070227636	18.5	21
GTTAGCC	50	0.0070227636	18.5	28
>>END_MODULE