FastQCFastQC Report
Fri 10 Feb 2017
ERR1632826.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632826.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236867
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6990.29510231480113314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.22755385933878508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2880.12158721983222653No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC2460.10385575027336015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1050.033.476191
GCCGGTA303.5909313E-430.83333231
TACGCAC303.5909313E-430.83333223
CCGGTAC303.5909313E-430.83333232
GTACGCA303.5909313E-430.83333222
AGTACCG250.005487646429.5999985
GTCGCCA358.8485325E-426.42857212
CGCACGG358.8485325E-426.42857225
AGTACGC358.8485325E-426.42857221
TTGGCTA551.894403E-523.54545422
GGCCGGT400.001926687723.12500230
GTCTAAG400.001926687723.12500216
GTATCAA3450.022.521741
ACAGTCT450.003816979220.5555578
ACGCACG450.003816979220.55555724
CTAGGCC450.003816979220.5555573
TATCACC555.126543E-420.1818184
GTATTAT701.2140189E-418.51
ACGGGGG701.2140189E-418.515
CTGTTAA801.6078575E-518.51
Produced by FastQC (version 0.11.5)