FastQCFastQC Report
Fri 10 Feb 2017
ERR1632825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202825
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4260.21003327992111426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.1622088006902502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAC352.378691E-531.7142873
ATATATC250.00548580829.63
CTAATAG250.00548580829.64
GTATCAA2100.022.904761
GGTATCA806.902137E-720.81251
CGCAATA450.003815115420.55555528
CTCTAAA450.003815115420.5555551
GTGTATA555.123047E-420.1818181
GTCGCCC500.00701540918.537
ATTAGGG701.21292585E-418.53
CGTGCAA609.201106E-418.511
ACCCTAA609.201106E-418.53
TAACCGT609.201106E-418.57
ATATAGC500.00701540918.51
CCTCAGA500.00701540918.51
GCGATAG500.00701540918.57
CGATAGA500.00701540918.58
TCTGCAC650.00157387617.07692310
TAGGCGA650.00157387617.0769234
CTGCTCG803.3654156E-416.18759
Produced by FastQC (version 0.11.5)