##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632825.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	202825
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18729939603106	33.0	33.0	33.0	27.0	33.0
2	30.841474177246393	33.0	33.0	33.0	27.0	33.0
3	31.290950326636263	33.0	33.0	33.0	27.0	33.0
4	31.454450881301614	33.0	33.0	33.0	27.0	33.0
5	31.569431776161714	33.0	33.0	33.0	27.0	33.0
6	34.295229877973625	37.0	33.0	37.0	27.0	37.0
7	34.464143966473564	37.0	37.0	37.0	27.0	37.0
8	34.63671638111673	37.0	37.0	37.0	27.0	37.0
9	34.760364846542586	37.0	37.0	37.0	27.0	37.0
10	34.7496314556884	37.0	37.0	37.0	27.0	37.0
11	34.77257734500185	37.0	37.0	37.0	27.0	37.0
12	34.78030321705904	37.0	37.0	37.0	27.0	37.0
13	34.806103784050286	37.0	37.0	37.0	27.0	37.0
14	34.77635893011217	37.0	37.0	37.0	27.0	37.0
15	34.76082336990016	37.0	37.0	37.0	27.0	37.0
16	34.77765068408727	37.0	37.0	37.0	27.0	37.0
17	34.73845433255269	37.0	37.0	37.0	27.0	37.0
18	34.74654012079379	37.0	37.0	37.0	27.0	37.0
19	34.75064710957722	37.0	37.0	37.0	27.0	37.0
20	34.757648218907924	37.0	37.0	37.0	27.0	37.0
21	34.752165660051766	37.0	37.0	37.0	27.0	37.0
22	34.75321089609269	37.0	37.0	37.0	27.0	37.0
23	34.70586466165413	37.0	37.0	37.0	27.0	37.0
24	34.719447799827435	37.0	37.0	37.0	27.0	37.0
25	34.70865031431037	37.0	37.0	37.0	27.0	37.0
26	34.67078022926168	37.0	37.0	37.0	27.0	37.0
27	34.608894367065204	37.0	37.0	37.0	27.0	37.0
28	34.56882041168495	37.0	37.0	37.0	27.0	37.0
29	34.58970541106866	37.0	37.0	37.0	27.0	37.0
30	34.55094786145692	37.0	37.0	37.0	27.0	37.0
31	34.54746209786762	37.0	37.0	37.0	27.0	37.0
32	34.58273388389005	37.0	37.0	37.0	27.0	37.0
33	34.56386540120794	37.0	37.0	37.0	27.0	37.0
34	34.45017626032294	37.0	37.0	37.0	27.0	37.0
35	34.48652779489708	37.0	37.0	37.0	27.0	37.0
36	34.4588734130408	37.0	37.0	37.0	27.0	37.0
37	34.48397879945766	37.0	37.0	37.0	27.0	37.0
38	34.34928140022187	37.0	37.0	37.0	27.0	37.0
39	34.39901392826328	37.0	37.0	37.0	27.0	37.0
40	34.44005423394552	37.0	37.0	37.0	27.0	37.0
41	34.46661407617404	37.0	37.0	37.0	27.0	37.0
42	34.36693701466782	37.0	37.0	37.0	27.0	37.0
43	34.13071366941945	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	3.0
17	10.0
18	6.0
19	17.0
20	29.0
21	68.0
22	206.0
23	554.0
24	948.0
25	1567.0
26	2537.0
27	3720.0
28	5016.0
29	6407.0
30	8149.0
31	9907.0
32	12224.0
33	15390.0
34	21281.0
35	37037.0
36	77745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.667200788857386	21.619129791692345	13.197584124245038	25.516085295205226
2	15.929002834956243	23.413780352520646	37.03586835942315	23.621348453099962
3	17.819302354246272	26.90841858745224	30.191051398989277	25.081227659312216
4	13.147787501540737	18.472081843954147	38.536669542709234	29.843461111795882
5	14.140268704548257	37.16750893627511	35.64057685196598	13.05164550721065
6	30.03475902871934	39.74904474300505	17.46283742142241	12.7533588068532
7	26.550967582891655	32.12868236164181	23.988167139159376	17.33218291630716
8	24.234192037470727	36.35202761000863	20.83175150992235	18.5820288425983
9	25.514113151731788	15.298409959324541	20.28497473191175	38.902502157031925
10	14.06976457537286	28.750400591643043	34.86897571798349	22.310859115000618
11	34.07863922100333	23.47935412301245	24.431406384814494	18.01060027116973
12	22.55540490570689	25.59990139282633	30.813262664858872	21.031431036607913
13	27.475902871933933	22.493775422161963	26.24824355971897	23.782078146185135
14	21.5905337113275	21.046222112658697	27.59225933686676	29.770984839147047
15	23.408849993837052	29.718723037100947	23.737211882164427	23.135215086897574
16	23.115986688031555	28.72574879822507	25.241464316529026	22.91680019721435
17	22.307900899790457	27.961050166399602	26.597805990385805	23.133242943424133
18	22.287686429187723	28.12966843337853	27.803278688524593	21.77936644890916
19	23.860963885122644	26.805867126833476	27.26685566374954	22.06631332429434
20	23.378774805867128	27.353629976580795	27.302847282139776	21.9647479354123
21	22.76987550844324	27.311721927770243	27.402933563416738	22.515469000369777
22	22.46764452113891	27.296930851719463	27.06865524466905	23.166769382472573
23	22.772340687785036	27.32256871687415	27.351657833107357	22.553432762233452
24	22.935535560212006	27.843707629730062	26.123505485024033	23.097251325033895
25	22.987797362258107	27.44484161222729	27.258967089855783	22.308393935658817
26	22.853198570195982	27.21705904104524	27.361518550474546	22.568223838284236
27	22.84432392456551	27.39159373844447	27.056822383828422	22.707259953161593
28	22.440527548379144	27.413780352520646	27.33390854184642	22.81178355725379
29	23.583384691236287	27.16233205965734	27.080981141378036	22.173302107728336
30	23.263404412671022	27.140145445581165	27.26685566374954	22.329594477998274
31	23.079502033772954	27.08492542832491	27.1652902748675	22.670282263034636
32	22.068285467767783	27.194379391100703	27.470479477381982	23.26685566374954
33	22.29951929002835	27.25798101811907	27.76778010600271	22.67471958584987
34	22.850733390854185	26.984346111179587	27.33242943424134	22.832491063724884
35	22.635276716381117	27.61444595094293	27.246641193146804	22.503636139529153
36	23.32454086034759	27.294958708246025	26.705780845556514	22.67471958584987
37	22.761000862812768	27.035621841488965	27.184025637865155	23.019351657833106
38	22.961173425366695	27.31320103537532	26.84974731911747	22.875878220140518
39	22.654012079378774	27.38567730802416	27.41821767533588	22.542092937261184
40	22.67521262171823	27.729323308270676	27.21163564649328	22.38382842351781
41	22.503636139529153	26.785159620362382	27.309256748428446	23.40194749168002
42	22.46074201898188	27.585849870578084	27.501540737088625	22.451867373351412
43	23.01540737088623	26.524343646000247	27.653395784543328	22.806853198570195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	11.5
2	17.0
3	36.0
4	55.0
5	55.0
6	62.5
7	70.0
8	63.0
9	56.0
10	88.0
11	120.0
12	120.0
13	180.0
14	240.0
15	446.0
16	652.0
17	789.0
18	926.0
19	926.0
20	973.5
21	1021.0
22	1301.0
23	1581.0
24	2126.0
25	2671.0
26	2671.0
27	3453.5
28	4236.0
29	5213.0
30	6190.0
31	7442.0
32	8694.0
33	8694.0
34	9830.0
35	10966.0
36	11884.5
37	12803.0
38	14113.0
39	15423.0
40	15423.0
41	16241.0
42	17059.0
43	17227.5
44	17396.0
45	16928.0
46	16460.0
47	16460.0
48	15919.5
49	15379.0
50	15224.5
51	15070.0
52	14444.5
53	13819.0
54	13819.0
55	12731.5
56	11644.0
57	10111.0
58	8578.0
59	7898.5
60	7219.0
61	7219.0
62	6285.5
63	5352.0
64	4389.5
65	3427.0
66	2839.5
67	2252.0
68	2252.0
69	1841.5
70	1431.0
71	1112.5
72	794.0
73	657.0
74	520.0
75	520.0
76	398.0
77	276.0
78	226.5
79	177.0
80	136.0
81	95.0
82	95.0
83	83.0
84	71.0
85	63.0
86	55.0
87	34.0
88	13.0
89	13.0
90	11.0
91	9.0
92	5.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	202825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.20670612861131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.39287485744309	50.429964784909664
2	9.033221551091424	11.057874737445825
3	3.0131169414075054	5.532688894916079
4	1.567564107443592	3.8378174264823524
5	0.9929897444491974	3.0388815738613437
6	0.5994888062213409	2.201564111386898
7	0.3990635235049585	1.7097754666871314
8	0.3286883310013347	1.609434406680193
9	0.25639964059738724	1.4124039708173253
>10	1.331458505445863	14.347813562566577
>50	0.06236559834699709	2.6708231881376143
>100	0.022768393047316395	2.150957876109014
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.21003327992111426	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.1622088006902502	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	9.860717367188463E-4	0.0	0.0
2	0.0	0.0	9.860717367188463E-4	0.0	0.0
3	0.0	0.0	9.860717367188463E-4	0.0	0.0
4	0.0	0.0	9.860717367188463E-4	0.0	0.0
5	0.0	0.0	0.0014791076050782695	0.0	0.0
6	0.0	0.0	0.0019721434734376927	0.0	0.0
7	0.0	0.0	0.002465179341797116	0.0	0.0
8	0.0	0.0	0.002465179341797116	9.860717367188463E-4	0.0
9	0.0	0.0	0.003451251078515962	0.0014791076050782695	0.0
10	0.0	0.0	0.003944286946875385	0.0014791076050782695	0.0
11	0.0	0.0	0.0054233945519536544	0.0014791076050782695	0.0
12	0.0	0.0	0.0054233945519536544	0.0014791076050782695	0.0
13	0.0	0.0	0.0054233945519536544	0.0014791076050782695	0.0
14	0.0	0.0	0.005916430420313078	0.0014791076050782695	0.0
15	0.0	0.0	0.007395538025391347	0.0014791076050782695	0.0
16	0.0	0.0	0.007395538025391347	0.0014791076050782695	0.0
17	0.0	0.0	0.007395538025391347	0.0014791076050782695	0.0
18	0.0	0.0	0.007395538025391347	0.0014791076050782695	0.0
19	0.0	0.0	0.007395538025391347	0.0014791076050782695	0.0
20	0.0	0.0	0.007395538025391347	0.0019721434734376927	0.0
21	0.0	0.0	0.008381609762110194	0.0019721434734376927	0.0
22	0.0	0.0	0.008381609762110194	0.0019721434734376927	0.0
23	0.0	0.0	0.008381609762110194	0.0019721434734376927	0.0
24	0.0	0.0	0.008381609762110194	0.0019721434734376927	0.0
25	0.0	0.0	0.008381609762110194	0.0019721434734376927	0.0
26	0.0	0.0	0.008381609762110194	0.0019721434734376927	0.0
27	0.0	0.0	0.008381609762110194	0.002958215210156539	4.930358683594232E-4
28	0.0	0.0	0.008381609762110194	0.006902502157031924	4.930358683594232E-4
29	0.0	0.0	0.008381609762110194	0.0192283988660175	4.930358683594232E-4
30	0.0	0.0	0.008381609762110194	0.04141501294219154	4.930358683594232E-4
31	0.0	0.0	0.008381609762110194	0.08578824109453963	4.930358683594232E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCAC	35	2.378691E-5	31.714287	3
ATATATC	25	0.005485808	29.6	3
CTAATAG	25	0.005485808	29.6	4
GTATCAA	210	0.0	22.90476	1
GGTATCA	80	6.902137E-7	20.8125	1
CGCAATA	45	0.0038151154	20.555555	28
CTCTAAA	45	0.0038151154	20.555555	1
GTGTATA	55	5.123047E-4	20.181818	1
GTCGCCC	50	0.007015409	18.5	37
ATTAGGG	70	1.21292585E-4	18.5	3
CGTGCAA	60	9.201106E-4	18.5	11
ACCCTAA	60	9.201106E-4	18.5	3
TAACCGT	60	9.201106E-4	18.5	7
ATATAGC	50	0.007015409	18.5	1
CCTCAGA	50	0.007015409	18.5	1
GCGATAG	50	0.007015409	18.5	7
CGATAGA	50	0.007015409	18.5	8
TCTGCAC	65	0.001573876	17.076923	10
TAGGCGA	65	0.001573876	17.076923	4
CTGCTCG	80	3.3654156E-4	16.1875	9
>>END_MODULE