FastQCFastQC Report
Fri 10 Feb 2017
ERR1632824.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632824.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294791
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9270.31446007510405677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6490.22015597491103867No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4920.1668979039387227No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4710.15977421291694793No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4000.1356893527957095No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3870.13127944882984896No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3840.1302617786838811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3530.11974585384221363No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3510.1190674070782351No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3430.1163536200223209No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3390.1149967264943638No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3300.11194371605646034No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3090.10482002503468558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1950.028.461541
TAACGGC802.7084752E-823.12500236
GTTGTAT400.001927800223.1250021
CACATGT855.147922E-821.76470628
ATCGGGA705.080714E-621.14285921
TAGACCA450.003819162520.5555574
GTCTATA450.003819162520.5555571
CCCCAAT450.003819162520.5555571
CATGCTA1650.020.1818184
TCTATTA555.1306334E-420.18181824
TCGGGAT759.2275E-619.73333222
TTAACGG851.2388919E-619.58823435
GTGACAC951.6655758E-719.47368424
ACCATCG801.6100343E-518.518
TTTAACG902.1411888E-618.534
GTGTAAG801.6100343E-518.51
GTATCAA4100.018.51
TGCTCGC500.0070227818.510
TATAGTG500.0070227818.55
ACATGTT1054.7674257E-717.61904729
Produced by FastQC (version 0.11.5)