##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632824.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	294791
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75671238267111	33.0	33.0	33.0	27.0	33.0
2	31.285001916612106	33.0	33.0	33.0	27.0	33.0
3	31.70869192071671	33.0	33.0	33.0	27.0	33.0
4	31.84376727919102	33.0	33.0	33.0	27.0	33.0
5	31.947260940802128	33.0	33.0	33.0	33.0	33.0
6	34.88684525646984	37.0	37.0	37.0	27.0	37.0
7	35.042542004335274	37.0	37.0	37.0	33.0	37.0
8	35.21631935846074	37.0	37.0	37.0	33.0	37.0
9	35.298326611056645	37.0	37.0	37.0	33.0	37.0
10	35.30848635134723	37.0	37.0	37.0	33.0	37.0
11	35.345794138898405	37.0	37.0	37.0	33.0	37.0
12	35.30913087577301	37.0	37.0	37.0	33.0	37.0
13	35.34468148620548	37.0	37.0	37.0	33.0	37.0
14	35.3225844751027	37.0	37.0	37.0	33.0	37.0
15	35.33674365906693	37.0	37.0	37.0	33.0	37.0
16	35.354495218646434	37.0	37.0	37.0	33.0	37.0
17	35.30205128379089	37.0	37.0	37.0	33.0	37.0
18	35.298404632434504	37.0	37.0	37.0	33.0	37.0
19	35.31067773439488	37.0	37.0	37.0	33.0	37.0
20	35.313951240031074	37.0	37.0	37.0	33.0	37.0
21	35.32722844320213	37.0	37.0	37.0	33.0	37.0
22	35.333877221489125	37.0	37.0	37.0	33.0	37.0
23	35.32315776261826	37.0	37.0	37.0	33.0	37.0
24	35.31104748788125	37.0	37.0	37.0	33.0	37.0
25	35.295792612393186	37.0	37.0	37.0	33.0	37.0
26	35.26292525891225	37.0	37.0	37.0	33.0	37.0
27	35.21201122150948	37.0	37.0	37.0	33.0	37.0
28	35.200243562388266	37.0	37.0	37.0	33.0	37.0
29	35.226947227018464	37.0	37.0	37.0	33.0	37.0
30	35.19669528581266	37.0	37.0	37.0	33.0	37.0
31	35.16999501341628	37.0	37.0	37.0	33.0	37.0
32	35.20107805190796	37.0	37.0	37.0	33.0	37.0
33	35.188957600469486	37.0	37.0	37.0	33.0	37.0
34	35.13887466035259	37.0	37.0	37.0	33.0	37.0
35	35.1233585828604	37.0	37.0	37.0	33.0	37.0
36	35.108592189042405	37.0	37.0	37.0	33.0	37.0
37	35.13493627688769	37.0	37.0	37.0	33.0	37.0
38	34.98410399231998	37.0	37.0	37.0	27.0	37.0
39	35.079880321990835	37.0	37.0	37.0	27.0	37.0
40	35.07731240098918	37.0	37.0	37.0	33.0	37.0
41	35.1227276273699	37.0	37.0	37.0	33.0	37.0
42	35.03692785736335	37.0	37.0	37.0	27.0	37.0
43	34.821212316522555	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	11.0
17	9.0
18	15.0
19	9.0
20	16.0
21	33.0
22	154.0
23	318.0
24	722.0
25	1287.0
26	2249.0
27	3393.0
28	4773.0
29	6578.0
30	8760.0
31	11149.0
32	14266.0
33	18846.0
34	26982.0
35	50357.0
36	144853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09247229393028	21.410422977635	13.774504649056448	24.72260007937827
2	15.716219287563051	23.14860358694804	38.34988178065138	22.78529534483753
3	17.098893792551333	27.64195650477796	30.471757957332485	24.787391745338223
4	12.310416532390745	18.33807680695815	39.93168041086736	29.419826249783743
5	13.41051796018196	37.51810604801368	36.12796862862163	12.943407363182729
6	28.68031927704713	40.867258498393774	17.687446360302722	12.764975864256373
7	25.76028440488346	32.585798073889634	24.42374427984572	17.230173241381184
8	23.552279411515276	36.5560685366921	21.128867570583907	18.762784481208723
9	25.315223327713532	15.535752448344759	20.97316403825083	38.17586018569088
10	14.537757258532316	28.402156103815923	34.97698369353203	22.08310294411973
11	33.35040757689346	24.094358375934135	24.620154618017512	17.935079429154893
12	22.361944564114918	26.37733173672195	30.543334090932216	20.717389608230917
13	27.110732688582758	22.687938234206605	26.645318208493475	23.55601086871716
14	21.334776163451394	21.235722935910527	28.09108826253176	29.33841263810632
15	23.35790441363542	29.161338032707917	24.952593532366997	22.52816402128966
16	22.91114721955555	28.78174706826192	25.612722233718127	22.694383478464403
17	21.91620504018101	28.247809465010803	26.762350275279772	23.073635219528413
18	22.145180823023768	28.295979185253284	28.00153328968659	21.557306702036357
19	23.654385649494046	26.947905465227905	27.80139149431292	21.596317390965126
20	23.131981641230567	27.144994250163673	27.94590065504035	21.77712345356541
21	22.104813240567044	27.830225481782005	27.911299870077443	22.1536614075735
22	22.12618431363237	27.48014695156908	27.771539836697862	22.622128898100687
23	22.206919478545817	27.556132989134674	28.275286558951933	21.96166097336757
24	22.4637115787117	28.276982675861884	26.257925106261727	23.001380639164694
25	22.580743645497996	27.576486392054033	27.860416362779056	21.98235359966892
26	22.51357741586412	27.80851518533469	27.619228538184682	22.058678860616503
27	22.447428856376213	27.549687744876877	27.074096563327917	22.92878683541899
28	22.20047423428802	27.25218883887229	28.356700170629363	22.19063675621033
29	23.515982509642424	27.25049272196234	27.177898918216638	22.0556258501786
30	22.95287169554023	27.22199795787524	27.552062308550806	22.273068038033724
31	22.6343409398523	27.3529381833231	27.772218283461843	22.240502593362756
32	21.636345750039858	27.346492939065303	27.82751169472609	23.189649616168744
33	21.721151595537176	27.293234868092988	28.495781757244963	22.489831779124874
34	22.721521349023547	26.847156120777093	28.09007059238579	22.34125193781357
35	22.21913152029743	27.896713264651908	27.547991627966933	22.33616358708373
36	23.17608068088917	27.3909312021059	27.164669206319054	22.268318910685874
37	22.230665115285067	26.9828454735728	27.511016279330104	23.27547313181203
38	22.59736559121547	27.63551126052016	27.187736396294326	22.57938675197004
39	21.987441950398757	27.61447941083683	27.929278709322876	22.468799929441534
40	22.27646027185362	27.96387949428578	28.069717189466438	21.689943044394163
41	22.255089198788294	26.902110308659356	27.80105227093093	23.04174822162142
42	22.067837891930214	27.839384513095716	27.649758642563715	22.443018952410352
43	22.82159224671038	26.5496572147725	27.881448212462388	22.74730232605473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	11.5
2	20.0
3	56.5
4	93.0
5	93.0
6	121.0
7	149.0
8	138.5
9	128.0
10	184.0
11	240.0
12	240.0
13	371.0
14	502.0
15	882.0
16	1262.0
17	1529.0
18	1796.0
19	1796.0
20	1813.5
21	1831.0
22	2147.5
23	2464.0
24	3336.5
25	4209.0
26	4209.0
27	5466.5
28	6724.0
29	7911.5
30	9099.0
31	10836.0
32	12573.0
33	12573.0
34	14681.0
35	16789.0
36	18550.5
37	20312.0
38	22070.0
39	23828.0
40	23828.0
41	24728.5
42	25629.0
43	25890.0
44	26151.0
45	25294.0
46	24437.0
47	24437.0
48	23046.0
49	21655.0
50	21197.5
51	20740.0
52	19216.5
53	17693.0
54	17693.0
55	16913.5
56	16134.0
57	13683.0
58	11232.0
59	10407.0
60	9582.0
61	9582.0
62	8416.0
63	7250.0
64	5905.0
65	4560.0
66	3790.0
67	3020.0
68	3020.0
69	2463.0
70	1906.0
71	1527.0
72	1148.0
73	934.5
74	721.0
75	721.0
76	557.0
77	393.0
78	302.0
79	211.0
80	171.0
81	131.0
82	131.0
83	102.5
84	74.0
85	64.5
86	55.0
87	41.0
88	27.0
89	27.0
90	22.0
91	17.0
92	10.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	294791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.99717829637675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21279322601379	56.453698561066204
2	6.2770400018521775	8.034256963440457
3	1.9293062062497885	3.7041045980902187
4	0.8920448701112591	2.28353418403514
5	0.5370074117738668	1.7183479538883981
6	0.39696935703515274	1.524295123222603
7	0.26379946806842625	1.1817695114725095
8	0.17723521551620486	0.9074042950229857
9	0.1629938857776621	0.9388033892399079
>10	0.9961608887040585	12.37102411316012
>50	0.11000838509215544	4.877822447346039
>100	0.043578200857762046	5.468929365034466
>500	0.0010628829477502938	0.5360094949809899
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.31446007510405677	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	649	0.22015597491103867	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	492	0.1668979039387227	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	471	0.15977421291694793	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	400	0.1356893527957095	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	387	0.13127944882984896	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	384	0.1302617786838811	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.11974585384221363	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	351	0.1190674070782351	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	343	0.1163536200223209	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	339	0.1149967264943638	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	330	0.11194371605646034	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	309	0.10482002503468558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0010176701459678212	0.0	0.0
4	0.0	0.0	0.0010176701459678212	0.0	0.0
5	0.0	0.0	0.0010176701459678212	0.0	0.0
6	0.0	0.0	0.001356893527957095	0.0	0.0
7	0.0	0.0	0.0033922338198927376	0.0	0.0
8	0.0	0.0	0.004070680583871285	0.0	0.0
9	0.0	0.0	0.00542757411182838	3.3922338198927375E-4	0.0
10	3.3922338198927375E-4	0.0	0.005766797493817654	3.3922338198927375E-4	0.0
11	3.3922338198927375E-4	0.0	0.007462914403764022	3.3922338198927375E-4	0.0
12	3.3922338198927375E-4	0.0	0.007462914403764022	6.784467639785475E-4	0.0
13	3.3922338198927375E-4	0.0	0.00814136116774257	6.784467639785475E-4	0.0
14	3.3922338198927375E-4	0.0	0.00915903131371039	6.784467639785475E-4	0.0
15	3.3922338198927375E-4	0.0	0.009837478077688939	6.784467639785475E-4	0.0
16	3.3922338198927375E-4	0.0	0.01085514822365676	6.784467639785475E-4	0.0
17	3.3922338198927375E-4	0.0	0.01085514822365676	6.784467639785475E-4	0.0
18	3.3922338198927375E-4	0.0	0.011194371605646033	6.784467639785475E-4	0.0
19	3.3922338198927375E-4	0.0	0.011194371605646033	6.784467639785475E-4	0.0
20	3.3922338198927375E-4	0.0	0.012212041751613856	6.784467639785475E-4	0.0
21	3.3922338198927375E-4	0.0	0.012890488515592402	0.0010176701459678212	0.0
22	3.3922338198927375E-4	0.0	0.013229711897581677	0.0020353402919356424	0.0
23	3.3922338198927375E-4	0.0	0.013229711897581677	0.0020353402919356424	0.0
24	3.3922338198927375E-4	0.0	0.013229711897581677	0.00271378705591419	0.0
25	3.3922338198927375E-4	0.0	0.013229711897581677	0.003053010437903464	0.0
26	3.3922338198927375E-4	0.0	0.013229711897581677	0.003053010437903464	0.0
27	3.3922338198927375E-4	0.0	0.013229711897581677	0.004749127347849833	0.0
28	3.3922338198927375E-4	0.0	0.013229711897581677	0.012890488515592402	0.0
29	3.3922338198927375E-4	0.0	0.013229711897581677	0.024424083503227712	0.0
30	3.3922338198927375E-4	0.0	0.013229711897581677	0.045455933186562686	0.0
31	3.3922338198927375E-4	0.0	0.013229711897581677	0.10515924841667486	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	195	0.0	28.46154	1
TAACGGC	80	2.7084752E-8	23.125002	36
GTTGTAT	40	0.0019278002	23.125002	1
CACATGT	85	5.147922E-8	21.764706	28
ATCGGGA	70	5.080714E-6	21.142859	21
TAGACCA	45	0.0038191625	20.555557	4
GTCTATA	45	0.0038191625	20.555557	1
CCCCAAT	45	0.0038191625	20.555557	1
CATGCTA	165	0.0	20.181818	4
TCTATTA	55	5.1306334E-4	20.181818	24
TCGGGAT	75	9.2275E-6	19.733332	22
TTAACGG	85	1.2388919E-6	19.588234	35
GTGACAC	95	1.6655758E-7	19.473684	24
ACCATCG	80	1.6100343E-5	18.5	18
TTTAACG	90	2.1411888E-6	18.5	34
GTGTAAG	80	1.6100343E-5	18.5	1
GTATCAA	410	0.0	18.5	1
TGCTCGC	50	0.00702278	18.5	10
TATAGTG	50	0.00702278	18.5	5
ACATGTT	105	4.7674257E-7	17.619047	29
>>END_MODULE