FastQCFastQC Report
Fri 10 Feb 2017
ERR1632823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383660
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9700.2528280248136371No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6980.1819319188865141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.18141062398999114No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5650.14726580826773705No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC5620.14648386592295262No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4960.12928113433769484No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG4880.12719595475160297No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4750.12380753792420372No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4730.12328624302768076No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG4570.11911588385549705No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4210.10973257571808373No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4040.10530156909763853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3990.10399833185633113No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT3870.10087056247719335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT303.5950495E-430.83333225
CCGTCCG303.5950495E-430.83333228
TTAGGTT250.00549183829.5999984
GGCGTCA250.00549183829.59999825
GGTCTAA250.00549183829.59999819
CGGCGAG405.9299742E-527.75000218
CGAGTCG405.9299742E-527.75000221
GTCCGGC405.9299742E-527.75000215
GCCGTCC405.9299742E-527.75000227
TTAGTAC358.8585855E-426.428573
CGTCCGC358.8585855E-426.4285729
GTATCAA5900.025.7118641
GGTATCA1850.024.01
TTGTCCG551.8983783E-523.54545413
CCTCGTA400.001928855123.12500222
ATAAGAC400.001928855123.1250023
AGTCGCC502.6975584E-422.19999923
TCCGAAT705.0873186E-621.14285717
TCCGCCG450.003821232620.55555731
GTCCGCC450.003821232620.55555730
Produced by FastQC (version 0.11.5)