##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632823.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	383660
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731858937601	33.0	33.0	33.0	27.0	33.0
2	31.247456080904968	33.0	33.0	33.0	27.0	33.0
3	31.68809883751238	33.0	33.0	33.0	27.0	33.0
4	31.829719022050774	33.0	33.0	33.0	27.0	33.0
5	31.93705364124485	33.0	33.0	33.0	33.0	33.0
6	34.824618151488295	37.0	37.0	37.0	27.0	37.0
7	35.010186102278055	37.0	37.0	37.0	33.0	37.0
8	35.186339467236614	37.0	37.0	37.0	33.0	37.0
9	35.275712870770995	37.0	37.0	37.0	33.0	37.0
10	35.298561226085596	37.0	37.0	37.0	33.0	37.0
11	35.31923838815618	37.0	37.0	37.0	33.0	37.0
12	35.274816243548976	37.0	37.0	37.0	33.0	37.0
13	35.30928686858156	37.0	37.0	37.0	33.0	37.0
14	35.28111348589897	37.0	37.0	37.0	33.0	37.0
15	35.304360631809416	37.0	37.0	37.0	33.0	37.0
16	35.29751863629255	37.0	37.0	37.0	33.0	37.0
17	35.25022155033102	37.0	37.0	37.0	33.0	37.0
18	35.253521347026016	37.0	37.0	37.0	33.0	37.0
19	35.28152009591826	37.0	37.0	37.0	33.0	37.0
20	35.282500130323726	37.0	37.0	37.0	33.0	37.0
21	35.27293176249805	37.0	37.0	37.0	33.0	37.0
22	35.271938695720166	37.0	37.0	37.0	33.0	37.0
23	35.27202210290361	37.0	37.0	37.0	33.0	37.0
24	35.265870823124644	37.0	37.0	37.0	33.0	37.0
25	35.25439451597769	37.0	37.0	37.0	33.0	37.0
26	35.21931918886514	37.0	37.0	37.0	33.0	37.0
27	35.17832716467706	37.0	37.0	37.0	33.0	37.0
28	35.149546473440026	37.0	37.0	37.0	33.0	37.0
29	35.18994422144607	37.0	37.0	37.0	33.0	37.0
30	35.1416488557577	37.0	37.0	37.0	33.0	37.0
31	35.136759109628315	37.0	37.0	37.0	33.0	37.0
32	35.17249648125945	37.0	37.0	37.0	33.0	37.0
33	35.15077672939582	37.0	37.0	37.0	33.0	37.0
34	35.08233331595684	37.0	37.0	37.0	33.0	37.0
35	35.08976958765574	37.0	37.0	37.0	33.0	37.0
36	35.08657926288902	37.0	37.0	37.0	33.0	37.0
37	35.11144763592765	37.0	37.0	37.0	33.0	37.0
38	34.949556899337956	37.0	37.0	37.0	27.0	37.0
39	35.03100922691967	37.0	37.0	37.0	27.0	37.0
40	35.05669863942032	37.0	37.0	37.0	27.0	37.0
41	35.08710055778554	37.0	37.0	37.0	33.0	37.0
42	35.01069436480217	37.0	37.0	37.0	27.0	37.0
43	34.79281655632592	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	4.0
16	18.0
17	18.0
18	17.0
19	26.0
20	22.0
21	78.0
22	180.0
23	457.0
24	929.0
25	1814.0
26	2948.0
27	4591.0
28	6513.0
29	8744.0
30	11616.0
31	14713.0
32	19106.0
33	24867.0
34	35584.0
35	65018.0
36	186396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20174112495439	21.280300265860397	13.396496898295366	25.121461710889854
2	16.056404107803786	22.872334879841528	37.717770943022465	23.35349006933222
3	17.175102955742062	27.180055257259035	30.201741124954385	25.44310066204452
4	12.431058749934838	18.25470468644112	39.74925715477246	29.56497940885159
5	13.588072772767553	37.42323932648699	35.86561017567638	13.123077725069072
6	29.21128082156076	40.4188604493562	17.529062190481152	12.840796538601888
7	26.640515039357766	32.10524943960799	23.985560131366316	17.268675389667933
8	24.114059323359225	36.07908043580254	20.762393786164836	19.044466454673408
9	25.641192722723243	15.505656049627275	20.583068341760935	38.27008288588855
10	14.827972684147422	28.090496794036383	34.323359224313194	22.758171297502997
11	33.65088880779857	23.78434030130845	24.423187196997343	18.141583693895637
12	22.887713079288954	26.180472293176248	29.994005108689986	20.937809518844812
13	27.37684408069645	22.567898660272114	26.154928843246623	23.90032841578481
14	21.825314080175154	20.965438148360526	27.63045404785487	29.578793723609447
15	23.873742376062136	28.887296043371734	24.432049210238233	22.806912370327893
16	22.972162852525674	28.541938174425276	25.342230099567324	23.14366887348173
17	22.242610644841786	27.76807590053693	26.42652348433509	23.56278997028619
18	22.44721889172705	28.010999322316632	27.65912526716364	21.882656518792682
19	24.39634050982641	26.578480946671533	27.2201949642913	21.80498357921076
20	23.711098368346974	26.75546056404108	27.494917374758902	22.038523692853047
21	22.694312672678933	27.44200594276182	27.51576917061982	22.347912213939424
22	22.517854350205912	27.310118333941514	27.08908929781578	23.082938018036803
23	22.641922535578377	27.122712818641503	27.93984256894125	22.295522076838868
24	22.767033310743887	27.877547828806755	26.004795913048014	23.350622947401344
25	22.921597247562946	27.389876453109522	27.390919042902567	22.29760725642496
26	22.941145806182558	27.56685607047907	27.245738414220927	22.246259709117446
27	22.842099775843195	27.511077516551115	26.589949434395038	23.056873273210655
28	22.378147317937756	27.14825626857113	27.836365531981443	22.63723088150967
29	24.027785017984673	26.980138664442478	26.633477558254704	22.358598759318145
30	23.468956888912057	26.65172287963301	27.274930928426212	22.604389303028725
31	23.058437157900226	27.059375488713965	27.206901944429962	22.675285408955848
32	22.032007506646508	27.120366991607153	27.555387582755564	23.292237918990775
33	22.21029036125736	27.02366678830214	27.963040191836523	22.803002658603972
34	23.02246781004014	26.672314028045662	27.61273002137309	22.692488140541105
35	22.810822082051814	27.737840796538606	27.13418130636501	22.317155815044572
36	23.715790022415682	27.2655476202888	26.459104415367772	22.55955794192775
37	22.731063962883805	26.647291873012563	27.200385758223426	23.42125840588021
38	23.003701193765313	27.23244539435959	26.830005734243862	22.933847677631235
39	22.3995204086952	27.179273314914248	27.428973570348745	22.992232706041808
40	22.432101339727883	27.840014596257102	27.449043423864882	22.278840640150133
41	22.43679299379659	26.610279935359433	27.433665224417453	23.519261846426524
42	22.386227388833863	27.534535786894647	27.24625970911745	22.832977115154044
43	23.142626283688685	26.098889641870404	27.42740968565918	23.331074388781733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	8.0
2	12.0
3	56.0
4	100.0
5	100.0
6	123.0
7	146.0
8	149.5
9	153.0
10	198.5
11	244.0
12	244.0
13	367.5
14	491.0
15	911.0
16	1331.0
17	1597.5
18	1864.0
19	1864.0
20	1783.5
21	1703.0
22	2106.0
23	2509.0
24	3482.0
25	4455.0
26	4455.0
27	5933.0
28	7411.0
29	9232.0
30	11053.0
31	13634.5
32	16216.0
33	16216.0
34	18556.0
35	20896.0
36	23234.0
37	25572.0
38	27786.0
39	30000.0
40	30000.0
41	31515.5
42	33031.0
43	33593.0
44	34155.0
45	32837.5
46	31520.0
47	31520.0
48	30195.0
49	28870.0
50	28275.5
51	27681.0
52	26058.5
53	24436.0
54	24436.0
55	23378.5
56	22321.0
57	19220.0
58	16119.0
59	14644.5
60	13170.0
61	13170.0
62	11599.5
63	10029.0
64	8340.0
65	6651.0
66	5536.0
67	4421.0
68	4421.0
69	3618.5
70	2816.0
71	2298.0
72	1780.0
73	1427.0
74	1074.0
75	1074.0
76	819.5
77	565.0
78	456.0
79	347.0
80	282.0
81	217.0
82	217.0
83	173.5
84	130.0
85	115.0
86	100.0
87	71.5
88	43.0
89	43.0
90	31.5
91	20.0
92	11.5
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	383660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.163139406268975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36392226211237	54.04614895802295
2	6.317611895195458	7.728099541210858
3	1.738227153743792	3.189462891725811
4	0.8497642893120759	2.078970067586543
5	0.5044556607356345	1.5427045950927571
6	0.36245299308323653	1.3301257766501662
7	0.2433653140000461	1.0419490638784672
8	0.18415275667006395	0.9010688582607882
9	0.1544533388132478	0.8502165984238533
>10	1.0774106614733494	12.56584893261243
>50	0.1263789482296097	5.290134771847292
>100	0.07565542479040283	8.517429852818987
>500	0.002149301840636444	0.9178400918690913
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.2528280248136371	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	698	0.1819319188865141	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.18141062398999114	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	565	0.14726580826773705	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	562	0.14648386592295262	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	496	0.12928113433769484	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	488	0.12719595475160297	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	475	0.12380753792420372	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	473	0.12328624302768076	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	457	0.11911588385549705	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	421	0.10973257571808373	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	404	0.10530156909763853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.10399833185633113	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	387	0.10087056247719335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.606474482614815E-4	0.0	0.0
2	0.0	0.0	2.606474482614815E-4	0.0	0.0
3	0.0	0.0	5.21294896522963E-4	0.0	0.0
4	0.0	0.0	5.21294896522963E-4	0.0	0.0
5	0.0	0.0	5.21294896522963E-4	0.0	0.0
6	0.0	0.0	0.001042589793045926	0.0	0.0
7	0.0	0.0	0.0023458270343533335	0.0	0.0
8	0.0	0.0	0.0023458270343533335	0.0	0.0
9	0.0	0.0	0.002867121930876297	2.606474482614815E-4	0.0
10	0.0	0.0	0.002867121930876297	2.606474482614815E-4	0.0
11	0.0	0.0	0.004431006620445186	2.606474482614815E-4	0.0
12	0.0	0.0	0.004952301516968149	2.606474482614815E-4	0.0
13	0.0	0.0	0.005734243861752594	2.606474482614815E-4	0.0
14	0.0	0.0	0.006255538758275557	2.606474482614815E-4	0.0
15	0.0	0.0	0.0072981285513214825	2.606474482614815E-4	0.0
16	0.0	0.0	0.008080070896105927	5.21294896522963E-4	2.606474482614815E-4
17	0.0	0.0	0.008080070896105927	5.21294896522963E-4	2.606474482614815E-4
18	0.0	0.0	0.00860136579262889	5.21294896522963E-4	2.606474482614815E-4
19	0.0	0.0	0.009122660689151853	5.21294896522963E-4	2.606474482614815E-4
20	0.0	0.0	0.009643955585674817	5.21294896522963E-4	2.606474482614815E-4
21	0.0	0.0	0.010165250482197779	7.819423447844446E-4	2.606474482614815E-4
22	0.0	0.0	0.010425897930459262	7.819423447844446E-4	2.606474482614815E-4
23	0.0	0.0	0.010425897930459262	0.0013032372413074077	2.606474482614815E-4
24	0.0	0.0	0.010425897930459262	0.0023458270343533335	2.606474482614815E-4
25	0.0	0.0	0.010425897930459262	0.0023458270343533335	2.606474482614815E-4
26	0.0	0.0	0.010425897930459262	0.0026064744826148154	2.606474482614815E-4
27	0.0	0.0	0.010425897930459262	0.0033884168273992598	2.606474482614815E-4
28	0.0	0.0	0.010425897930459262	0.00860136579262889	2.606474482614815E-4
29	0.0	0.0	0.010425897930459262	0.024240212688317782	2.606474482614815E-4
30	0.0	0.0	0.010425897930459262	0.048219777928374084	2.606474482614815E-4
31	0.0	0.0	0.010425897930459262	0.10582286399416149	2.606474482614815E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	30	3.5950495E-4	30.833332	25
CCGTCCG	30	3.5950495E-4	30.833332	28
TTAGGTT	25	0.005491838	29.599998	4
GGCGTCA	25	0.005491838	29.599998	25
GGTCTAA	25	0.005491838	29.599998	19
CGGCGAG	40	5.9299742E-5	27.750002	18
CGAGTCG	40	5.9299742E-5	27.750002	21
GTCCGGC	40	5.9299742E-5	27.750002	15
GCCGTCC	40	5.9299742E-5	27.750002	27
TTAGTAC	35	8.8585855E-4	26.42857	3
CGTCCGC	35	8.8585855E-4	26.42857	29
GTATCAA	590	0.0	25.711864	1
GGTATCA	185	0.0	24.0	1
TTGTCCG	55	1.8983783E-5	23.545454	13
CCTCGTA	40	0.0019288551	23.125002	22
ATAAGAC	40	0.0019288551	23.125002	3
AGTCGCC	50	2.6975584E-4	22.199999	23
TCCGAAT	70	5.0873186E-6	21.142857	17
TCCGCCG	45	0.0038212326	20.555557	31
GTCCGCC	45	0.0038212326	20.555557	30
>>END_MODULE