FastQCFastQC Report
Fri 10 Feb 2017
ERR1632822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251832
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7230.28709615934432475No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG5090.20211887289939326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.20132469265224437No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG4950.196559611169351No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT4690.1862352679564154No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT4400.17471965437275644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2790.11078814447727056No Hit
GATTATAACTGTAATGTGAGGTTACCCTATGTCTGCAAGTTCA2580.10244925188220717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA250.00548829529.616
GCACCGC250.00548829529.610
CCTATCG405.9220023E-527.75000219
GGTATCA1850.025.01
CGAACTA701.9105391E-723.78571529
GCGAACT701.9105391E-723.78571528
TAAGACT701.9105391E-723.7857159
AGTTTCA400.001927023823.12500227
TATTAGA400.001927023823.1250022
GTATCAA3950.022.9493681
GACGTAT1054.0017767E-1122.9047626
CCCAATC1054.0017767E-1122.9047617
ACGTATG1054.0017767E-1122.9047627
CATGCTA753.7168138E-722.24
CTATCGC502.6939894E-422.220
TCGCTCC502.6939894E-422.223
TATCGCT502.6939894E-422.221
AAAGCGA753.7168138E-722.225
GTCTAGA603.7114296E-521.5833341
TATACTG603.7114296E-521.5833345
Produced by FastQC (version 0.11.5)