##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632822.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	251832
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.710231424124018	33.0	33.0	33.0	27.0	33.0
2	31.22537644143715	33.0	33.0	33.0	27.0	33.0
3	31.658784427713712	33.0	33.0	33.0	27.0	33.0
4	31.78419740144223	33.0	33.0	33.0	27.0	33.0
5	31.880090695384226	33.0	33.0	33.0	33.0	33.0
6	34.790737475777505	37.0	37.0	37.0	27.0	37.0
7	34.96683503287906	37.0	37.0	37.0	27.0	37.0
8	35.127235617395726	37.0	37.0	37.0	33.0	37.0
9	35.2195273039169	37.0	37.0	37.0	33.0	37.0
10	35.248765049715686	37.0	37.0	37.0	33.0	37.0
11	35.28234299056514	37.0	37.0	37.0	33.0	37.0
12	35.25171542933384	37.0	37.0	37.0	33.0	37.0
13	35.25542028018679	37.0	37.0	37.0	33.0	37.0
14	35.25632167476731	37.0	37.0	37.0	33.0	37.0
15	35.26822246577083	37.0	37.0	37.0	33.0	37.0
16	35.257755170113406	37.0	37.0	37.0	33.0	37.0
17	35.21606864894056	37.0	37.0	37.0	33.0	37.0
18	35.21815337208933	37.0	37.0	37.0	33.0	37.0
19	35.245425521776426	37.0	37.0	37.0	33.0	37.0
20	35.25608342069316	37.0	37.0	37.0	33.0	37.0
21	35.234179929476795	37.0	37.0	37.0	33.0	37.0
22	35.25729057466883	37.0	37.0	37.0	33.0	37.0
23	35.250115156135834	37.0	37.0	37.0	33.0	37.0
24	35.2441230661711	37.0	37.0	37.0	33.0	37.0
25	35.228255344833066	37.0	37.0	37.0	33.0	37.0
26	35.182828234696146	37.0	37.0	37.0	33.0	37.0
27	35.12930842784078	37.0	37.0	37.0	33.0	37.0
28	35.103918485339435	37.0	37.0	37.0	33.0	37.0
29	35.14040709679469	37.0	37.0	37.0	33.0	37.0
30	35.11004161504495	37.0	37.0	37.0	33.0	37.0
31	35.10055116109152	37.0	37.0	37.0	33.0	37.0
32	35.13684916928746	37.0	37.0	37.0	33.0	37.0
33	35.09082642396518	37.0	37.0	37.0	33.0	37.0
34	35.04098367165412	37.0	37.0	37.0	27.0	37.0
35	35.04170240477779	37.0	37.0	37.0	27.0	37.0
36	35.02824899139109	37.0	37.0	37.0	27.0	37.0
37	35.04477588233426	37.0	37.0	37.0	27.0	37.0
38	34.89815035420439	37.0	37.0	37.0	27.0	37.0
39	34.972565043362245	37.0	37.0	37.0	27.0	37.0
40	35.00966120270657	37.0	37.0	37.0	27.0	37.0
41	35.018762508338895	37.0	37.0	37.0	27.0	37.0
42	34.92540662028654	37.0	37.0	37.0	27.0	37.0
43	34.74550493980114	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	10.0
17	18.0
18	10.0
19	12.0
20	23.0
21	41.0
22	145.0
23	383.0
24	683.0
25	1297.0
26	2075.0
27	3063.0
28	4373.0
29	5753.0
30	7903.0
31	9847.0
32	12645.0
33	16512.0
34	23553.0
35	42965.0
36	120509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.755217764223765	20.081244639283334	12.950697290256997	25.2128403062359
2	16.387909399917405	22.830299564789225	36.74711712570285	24.03467390959052
3	18.8323962006417	24.893579846882048	30.128418945963976	26.14560500651228
4	13.452222116331521	17.01253216430001	38.04441055942057	31.4908351599479
5	14.768575875980813	36.445725721909845	34.60759553988373	14.178102862225611
6	31.294672638902128	38.799278884335585	16.515772419708377	13.39027605705391
7	27.885653928015504	31.329219479653105	22.318450395501763	18.46667619682963
8	25.489215032243717	35.20442199561612	20.40685854061438	18.89950443152578
9	25.617475142158263	15.02271355506846	20.092363162743414	39.267448140029856
10	14.711394898186093	28.34310175037327	33.41076590743035	23.534737444010293
11	34.89469169922806	22.968884017916707	23.853600813240572	18.282823469614666
12	23.60502239588297	24.98054258394485	30.243575081800568	21.170859938371613
13	28.740588964071286	21.82089647066298	26.099145462054068	23.339369103211666
14	22.964516026557387	20.02485784173576	26.707487531370123	30.30313860033673
15	23.88616220337368	29.09717589504114	23.866307697194955	23.15035420439023
16	24.0164077639061	27.866593602083928	24.078750913307285	24.038247720702692
17	22.820769401823437	26.980685536389338	25.852949585437912	24.345595476349313
18	24.178817624448044	26.501397757234983	26.81112805362305	22.508656564693926
19	24.94996664442962	25.985180596588204	26.479557800438386	22.58529495854379
20	24.178023444200896	26.852028336351218	26.532370786873788	22.437577432574095
21	23.54347342672893	26.618142253565868	27.209012357444646	22.629371962260556
22	23.27027542170971	26.290145811493375	27.00848184503955	23.431096921757362
23	23.525207281044505	26.065789891673813	26.94693287588551	23.46206995139617
24	23.161472727850313	26.607023730105784	25.80172495949681	24.429778582547097
25	23.917135233012484	27.043425775914105	26.212713237396358	22.826725753677053
26	24.139108612090602	26.046332475618666	26.423965183137966	23.390593729152766
27	23.92031195400108	26.37234346707329	26.295307983099846	23.41203659582579
28	23.37471012420979	25.93673560151212	27.01126147590457	23.67729279837352
29	23.960020966358524	25.929587979287778	26.248054258394486	23.86233679595921
30	23.375901394580513	26.52085517329013	26.915959846246707	23.18728358588265
31	23.595492232917184	26.304441055942057	27.004510943803805	23.095555767336958
32	23.65862956256552	26.276644747291844	26.73329838940246	23.331427300740177
33	23.84168810953334	26.031640141046413	26.945344515391213	23.181327234029037
34	23.938975189809078	25.868039010133742	26.4918675942692	23.701118205787985
35	23.484704088439912	26.631246227643825	26.89173734870866	22.9923123352076
36	23.844070650274787	25.72985164712983	27.159773182121416	23.266304520473966
37	24.057308046634265	25.989151497823947	26.401331046094224	23.55220940944757
38	24.246322945455702	26.064201531179513	26.75831506718765	22.931160456177132
39	23.9262683058547	25.94070650274786	26.576447790590553	23.556577400806887
40	23.102306299437718	27.193128752501668	26.59868483751072	23.10588011054989
41	24.187156517043107	25.666317227357922	26.57763906096128	23.568887194637693
42	23.588344610692843	26.517678452301535	26.41046411893643	23.483512818069187
43	23.845659010769086	25.507481177928142	27.083928968518695	23.562930842784077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.0
2	14.0
3	52.5
4	91.0
5	91.0
6	97.0
7	103.0
8	106.5
9	110.0
10	137.5
11	165.0
12	165.0
13	250.0
14	335.0
15	623.0
16	911.0
17	1092.5
18	1274.0
19	1274.0
20	1252.0
21	1230.0
22	1427.5
23	1625.0
24	2136.0
25	2647.0
26	2647.0
27	3396.5
28	4146.0
29	5117.5
30	6089.0
31	7302.0
32	8515.0
33	8515.0
34	9975.5
35	11436.0
36	13014.5
37	14593.0
38	15349.0
39	16105.0
40	16105.0
41	16952.5
42	17800.0
43	19008.5
44	20217.0
45	20393.0
46	20569.0
47	20569.0
48	19876.0
49	19183.0
50	19472.0
51	19761.0
52	19300.0
53	18839.0
54	18839.0
55	17631.5
56	16424.0
57	14580.5
58	12737.0
59	12281.0
60	11825.0
61	11825.0
62	11132.0
63	10439.0
64	8198.0
65	5957.0
66	4817.5
67	3678.0
68	3678.0
69	2856.0
70	2034.0
71	1642.5
72	1251.0
73	1001.0
74	751.0
75	751.0
76	566.0
77	381.0
78	299.0
79	217.0
80	186.5
81	156.0
82	156.0
83	125.5
84	95.0
85	82.5
86	70.0
87	52.0
88	34.0
89	34.0
90	24.5
91	15.0
92	9.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	251832.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.483039611186776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.00633877426863	53.49414173282694
2	6.574118540768501	8.083935813014143
3	2.132541701849207	3.933454377819074
4	1.0556436554147568	2.59616722724654
5	0.6258436953806296	1.9239386356749377
6	0.4128301277840106	1.522923065954137
7	0.31518569226109533	1.3565002081517774
8	0.22796100145045664	1.1212588225587385
9	0.2027726886515282	1.1220373123585874
>10	1.2948818286108708	15.497169902176989
>50	0.10940673688667604	4.647329816158123
>100	0.04054484955212112	4.013045465770599
>500	0.0019307071215295772	0.6880976202893977
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.28709615934432475	No Hit
GCCTTAGGCCGTATGACAAAATGAAGAGACTGAAATGACAGCG	509	0.20211887289939326	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.20132469265224437	No Hit
ACCCCACACAGGGCACCGAGCCCAATGGAGAAGGTTGGGAGTG	495	0.196559611169351	No Hit
CTTTAAAGCCTTAGGCCGTATGACAAAATGAAGAGACTGAAAT	469	0.1862352679564154	No Hit
GTGTGGGGTCATGGAGCCCAATCCAGACGTATGAGTAGCTGTT	440	0.17471965437275644	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.11078814447727056	No Hit
GATTATAACTGTAATGTGAGGTTACCCTATGTCTGCAAGTTCA	258	0.10244925188220717	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	3.970901235744465E-4	0.0	0.0
5	0.0	0.0	3.970901235744465E-4	0.0	0.0
6	0.0	0.0	3.970901235744465E-4	0.0	0.0
7	0.0	0.0	3.970901235744465E-4	0.0	0.0
8	0.0	0.0	3.970901235744465E-4	0.0	0.0
9	0.0	0.0	3.970901235744465E-4	0.0	0.0
10	3.970901235744465E-4	0.0	0.0011912703707233393	0.0	0.0
11	3.970901235744465E-4	0.0	0.001588360494297786	0.0	0.0
12	3.970901235744465E-4	0.0	0.001588360494297786	0.0	0.0
13	3.970901235744465E-4	0.0	0.001588360494297786	0.0	0.0
14	3.970901235744465E-4	0.0	0.0019854506178722323	3.970901235744465E-4	0.0
15	3.970901235744465E-4	0.0	0.0023825407414466786	3.970901235744465E-4	0.0
16	3.970901235744465E-4	0.0	0.003573811112170018	3.970901235744465E-4	0.0
17	3.970901235744465E-4	0.0	0.003573811112170018	3.970901235744465E-4	0.0
18	3.970901235744465E-4	0.0	0.003573811112170018	3.970901235744465E-4	0.0
19	3.970901235744465E-4	0.0	0.0039709012357444645	3.970901235744465E-4	0.0
20	3.970901235744465E-4	0.0	0.0039709012357444645	3.970901235744465E-4	0.0
21	3.970901235744465E-4	0.0	0.0039709012357444645	3.970901235744465E-4	0.0
22	3.970901235744465E-4	0.0	0.0039709012357444645	7.94180247148893E-4	0.0
23	3.970901235744465E-4	0.0	0.0039709012357444645	7.94180247148893E-4	0.0
24	3.970901235744465E-4	0.0	0.0039709012357444645	0.001588360494297786	0.0
25	3.970901235744465E-4	0.0	0.0039709012357444645	0.001588360494297786	0.0
26	3.970901235744465E-4	0.0	0.0039709012357444645	0.001588360494297786	0.0
27	3.970901235744465E-4	0.0	0.0039709012357444645	0.0019854506178722323	0.0
28	3.970901235744465E-4	0.0	0.0039709012357444645	0.007544712347914482	0.0
29	3.970901235744465E-4	0.0	0.0039709012357444645	0.015883604942977858	0.0
30	3.970901235744465E-4	0.0	0.0039709012357444645	0.036135201245274626	0.0
31	3.970901235744465E-4	0.0	0.0039709012357444645	0.07425585310842149	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	25	0.005488295	29.6	16
GCACCGC	25	0.005488295	29.6	10
CCTATCG	40	5.9220023E-5	27.750002	19
GGTATCA	185	0.0	25.0	1
CGAACTA	70	1.9105391E-7	23.785715	29
GCGAACT	70	1.9105391E-7	23.785715	28
TAAGACT	70	1.9105391E-7	23.785715	9
AGTTTCA	40	0.0019270238	23.125002	27
TATTAGA	40	0.0019270238	23.125002	2
GTATCAA	395	0.0	22.949368	1
GACGTAT	105	4.0017767E-11	22.90476	26
CCCAATC	105	4.0017767E-11	22.90476	17
ACGTATG	105	4.0017767E-11	22.90476	27
CATGCTA	75	3.7168138E-7	22.2	4
CTATCGC	50	2.6939894E-4	22.2	20
TCGCTCC	50	2.6939894E-4	22.2	23
TATCGCT	50	2.6939894E-4	22.2	21
AAAGCGA	75	3.7168138E-7	22.2	25
GTCTAGA	60	3.7114296E-5	21.583334	1
TATACTG	60	3.7114296E-5	21.583334	5
>>END_MODULE