FastQCFastQC Report
Fri 10 Feb 2017
ERR1632821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392163
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18420.46970264915354076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13750.3506195128046246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6170.15733253774578426No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG4260.10862829996710552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2750.028.9272731
TCTATAC809.731593E-1025.43753
GTATCAA8550.025.0994151
ATACCGC400.001928931523.1256
CCGCTCT1059.767973E-1021.14285928
GCCGCTC1059.767973E-1021.14285927
CGCTCTC1059.767973E-1021.14285929
GCGGTAA450.00382138220.55555523
CGGTTCG450.00382138220.55555522
CGTATTA1001.283297E-820.3499982
TCTAATA1001.283297E-820.3499982
TTAGCTC555.134794E-420.1818184
GTCTACG555.134794E-420.1818181
AACTCCG555.134794E-420.1818185
CGGAGTT1101.7444108E-920.18181824
AGCTTCG1202.3646862E-1020.04166621
CGCGATA656.887321E-519.92307734
GCTTCGC1303.274181E-1119.92307722
TTCGCCG1254.0927262E-1019.24000224
CGGCAGC1409.276846E-1118.517
Produced by FastQC (version 0.11.5)