##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632821.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392163
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745786828436135	33.0	33.0	33.0	27.0	33.0
2	31.25326203644913	33.0	33.0	33.0	27.0	33.0
3	31.693515196487176	33.0	33.0	33.0	27.0	33.0
4	31.821665990927244	33.0	33.0	33.0	27.0	33.0
5	31.939277800302424	33.0	33.0	33.0	33.0	33.0
6	34.806218332683095	37.0	37.0	37.0	27.0	37.0
7	34.99607561141668	37.0	37.0	37.0	33.0	37.0
8	35.154874376216014	37.0	37.0	37.0	33.0	37.0
9	35.25954513811859	37.0	37.0	37.0	33.0	37.0
10	35.27855764057293	37.0	37.0	37.0	33.0	37.0
11	35.308139727613266	37.0	37.0	37.0	33.0	37.0
12	35.27992441918284	37.0	37.0	37.0	33.0	37.0
13	35.29535422770633	37.0	37.0	37.0	33.0	37.0
14	35.260927216489065	37.0	37.0	37.0	33.0	37.0
15	35.28609277264811	37.0	37.0	37.0	33.0	37.0
16	35.30582181388861	37.0	37.0	37.0	33.0	37.0
17	35.26782995846115	37.0	37.0	37.0	33.0	37.0
18	35.26635608152733	37.0	37.0	37.0	33.0	37.0
19	35.265448295734174	37.0	37.0	37.0	33.0	37.0
20	35.265167800123926	37.0	37.0	37.0	33.0	37.0
21	35.279934619023216	37.0	37.0	37.0	33.0	37.0
22	35.28362441127796	37.0	37.0	37.0	33.0	37.0
23	35.265524794536965	37.0	37.0	37.0	33.0	37.0
24	35.261615705714206	37.0	37.0	37.0	33.0	37.0
25	35.26408661704444	37.0	37.0	37.0	33.0	37.0
26	35.219492914935884	37.0	37.0	37.0	33.0	37.0
27	35.16783837332946	37.0	37.0	37.0	33.0	37.0
28	35.13402590249463	37.0	37.0	37.0	33.0	37.0
29	35.18349767826133	37.0	37.0	37.0	33.0	37.0
30	35.12789324847066	37.0	37.0	37.0	33.0	37.0
31	35.12056211320293	37.0	37.0	37.0	33.0	37.0
32	35.16368448833776	37.0	37.0	37.0	33.0	37.0
33	35.135446230266496	37.0	37.0	37.0	33.0	37.0
34	35.07185787542425	37.0	37.0	37.0	33.0	37.0
35	35.07301300734643	37.0	37.0	37.0	33.0	37.0
36	35.05140719547739	37.0	37.0	37.0	27.0	37.0
37	35.065597723395626	37.0	37.0	37.0	27.0	37.0
38	34.91312030966716	37.0	37.0	37.0	27.0	37.0
39	34.97615787312929	37.0	37.0	37.0	27.0	37.0
40	35.00105823343865	37.0	37.0	37.0	27.0	37.0
41	35.018780456085864	37.0	37.0	37.0	27.0	37.0
42	34.92099968635491	37.0	37.0	37.0	27.0	37.0
43	34.72144235942707	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	21.0
17	16.0
18	11.0
19	15.0
20	30.0
21	93.0
22	203.0
23	492.0
24	967.0
25	1896.0
26	3082.0
27	4736.0
28	6807.0
29	9242.0
30	11886.0
31	15147.0
32	19633.0
33	25666.0
34	35948.0
35	66213.0
36	190045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90110489770835	20.395090816828716	13.862858046271576	24.840946239191357
2	15.69296440510706	23.252321101174765	38.0153150603193	23.039399433398867
3	17.77551681316187	26.960218072587168	30.38863941779311	24.875625696457853
4	12.792384799177892	18.05346246331245	39.178351858793405	29.975800878716246
5	14.266771725022503	36.42668992230272	35.078271025058456	14.228267327616322
6	29.29011660967506	39.97138944775514	17.493491226862297	13.2450027157075
7	26.25081917467992	32.30365944772964	23.543016551790966	17.902504825799475
8	24.546680844444786	35.52935896553219	20.851278677488697	19.072681512534327
9	26.118731241856064	15.390794134071802	20.118420146724702	38.372054477347426
10	14.905536728350203	28.108975094539772	34.04247723523127	22.94301094187876
11	33.956543580092976	23.567496168684958	24.345998985115884	18.129961266106186
12	23.039144437389556	25.432281984786936	30.44881847599085	21.079755101832657
13	28.34790635526554	22.013805483944175	26.26407896716416	23.37420919362612
14	22.251971756642007	21.01983103964423	27.773400346284582	28.954796857429184
15	23.821217197950855	28.560318031022813	24.367163653888817	23.251301117137515
16	22.78287344803054	28.202813625966755	25.431516996759	23.58279592924371
17	22.418484150723042	27.31440752952216	26.577214066599858	23.68989425315494
18	23.38670399808243	26.80875044305557	27.705061415788844	22.099484143073163
19	24.706053350265066	26.15111573503875	27.473015047314508	21.669815867381676
20	24.33528915272476	26.20211493690124	27.118825590379508	22.34377031999449
21	23.305105275102445	26.914573786920236	27.26519329972486	22.515127638252462
22	23.06362405428355	26.439006229552508	27.407481072921207	23.089888643242734
23	22.989165219564313	26.635353156723095	27.71602624418928	22.659455379523312
24	23.07713884277711	26.707517027358524	27.13030041079857	23.085043719065794
25	23.44586307224292	26.446656109831878	27.72316613245003	22.384314685475175
26	23.25155611314683	26.58766890298167	27.261623355594484	22.899151628277018
27	23.2038718594054	26.82736515173538	27.16115492792538	22.807608060933845
28	23.01083988035587	26.55247945369655	27.5423739618475	22.89430670410008
29	23.43642821989836	26.35791749859115	27.087461081234075	23.118193200276416
30	23.321935011717066	26.601693683493856	27.29630281286098	22.780068491928102
31	23.377014149728556	26.453286006074006	27.27284318000423	22.896856664193205
32	22.573776720394324	26.44334116171082	27.571953498927744	23.410928618967112
33	22.682915012380057	26.527744840793243	27.76702544605177	23.02231470077493
34	23.039144437389556	26.227614537832483	27.563793626629746	23.16944739814822
35	22.809138036989722	26.79064572639438	27.46281520694201	22.937401029673886
36	23.30459528308382	26.471900714753815	27.36693670744053	22.856567294721838
37	23.21968161198277	26.19625002868705	27.498769644255066	23.08529871507511
38	23.04092940945474	26.670797602017526	27.060941496265585	23.227331492262145
39	22.73850414241017	26.49128041146156	27.533704097530876	23.236511348597393
40	22.89634667217458	26.53386474501674	27.844544232882757	22.725244349925923
41	23.05112924982724	26.107256421437004	27.527584193307376	23.314030135428382
42	22.49243299342365	27.0859311051782	27.534979077577436	22.886656823820708
43	22.900681604332892	26.093741632943445	27.70888635592853	23.296690406795133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	18.5
2	32.0
3	117.0
4	202.0
5	202.0
6	243.0
7	284.0
8	287.0
9	290.0
10	375.5
11	461.0
12	461.0
13	718.5
14	976.0
15	1705.5
16	2435.0
17	2747.0
18	3059.0
19	3059.0
20	2836.5
21	2614.0
22	2870.5
23	3127.0
24	4144.5
25	5162.0
26	5162.0
27	6640.5
28	8119.0
29	9778.5
30	11438.0
31	13234.0
32	15030.0
33	15030.0
34	17363.5
35	19697.0
36	21492.0
37	23287.0
38	24777.0
39	26267.0
40	26267.0
41	27609.5
42	28952.0
43	29832.0
44	30712.0
45	30871.0
46	31030.0
47	31030.0
48	30789.5
49	30549.0
50	29981.0
51	29413.0
52	28206.0
53	26999.0
54	26999.0
55	25148.5
56	23298.0
57	20928.0
58	18558.0
59	17221.5
60	15885.0
61	15885.0
62	13755.0
63	11625.0
64	9626.5
65	7628.0
66	6660.0
67	5692.0
68	5692.0
69	4672.5
70	3653.0
71	3017.5
72	2382.0
73	1949.0
74	1516.0
75	1516.0
76	1139.5
77	763.0
78	598.5
79	434.0
80	347.5
81	261.0
82	261.0
83	200.5
84	140.0
85	123.5
86	107.0
87	82.0
88	57.0
89	57.0
90	38.5
91	20.0
92	11.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	392163.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.04819370155644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.63474075728139	65.47655905226688
2	6.169257686426864	9.01306260945851
3	1.567175262879751	3.4343796650138305
4	0.723059880923392	2.1127287295806507
5	0.43147834006028996	1.5759356681375052
6	0.2838393220650665	1.2440369866996466
7	0.19069568235941384	0.9750982600128658
8	0.14909273265765854	0.8712763851736808
9	0.11596337203347908	0.762382337632851
>10	0.6670382327293717	9.004232087160998
>50	0.04732605507859542	2.374375709245881
>100	0.01928098539243787	2.174120983986229
>500	3.5056337077159764E-4	0.1580014896489403
>1k	7.011267415431953E-4	0.82381003598159
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1842	0.46970264915354076	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1375	0.3506195128046246	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	617	0.15733253774578426	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	426	0.10862829996710552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.099920186249085E-4	0.0	0.0
2	0.0	0.0	7.649880279373628E-4	0.0	0.0
3	0.0	0.0	0.001019984037249817	0.0	0.0
4	0.0	0.0	0.0015299760558747256	0.0	0.0
5	0.0	0.0	0.0017849720651871798	0.0	0.0
6	0.0	0.0	0.004334932158311723	0.0	0.0
7	0.0	0.0	0.009689848353873261	0.0	0.0
8	0.0	0.0	0.010964828400435533	0.0	0.0
9	0.0	0.0	0.012749800465622713	2.5499600931245425E-4	0.0
10	2.5499600931245425E-4	0.0	0.016064748586684618	2.5499600931245425E-4	0.0
11	2.5499600931245425E-4	0.0	0.024224620884683155	2.5499600931245425E-4	0.0
12	2.5499600931245425E-4	0.0	0.027284572996432607	2.5499600931245425E-4	0.0
13	2.5499600931245425E-4	0.0	0.030344525108182058	2.5499600931245425E-4	0.0
14	2.5499600931245425E-4	0.0	0.03110951313611942	2.5499600931245425E-4	0.0
15	2.5499600931245425E-4	0.0	0.0328944852013066	2.5499600931245425E-4	0.0
16	2.5499600931245425E-4	0.0	0.0356994413037436	2.5499600931245425E-4	0.0
17	2.5499600931245425E-4	0.0	0.0356994413037436	2.5499600931245425E-4	0.0
18	2.5499600931245425E-4	0.0	0.03646442933168096	5.099920186249085E-4	0.0
19	2.5499600931245425E-4	0.0	0.03722941735961832	7.649880279373628E-4	0.0
20	2.5499600931245425E-4	0.0	0.037994405387555684	0.001019984037249817	0.0
21	2.5499600931245425E-4	0.0	0.039269385434117954	0.0015299760558747256	0.0
22	2.5499600931245425E-4	0.0	0.04054436548068023	0.0017849720651871798	0.0
23	2.5499600931245425E-4	0.0	0.04130935350861759	0.002039968074499634	0.0
24	2.5499600931245425E-4	0.0	0.041564349517930047	0.0022949640838120884	0.0
25	2.5499600931245425E-4	0.0	0.041564349517930047	0.0022949640838120884	0.0
26	2.5499600931245425E-4	0.0	0.041564349517930047	0.003059952111749451	0.0
27	2.5499600931245425E-4	0.0	0.041564349517930047	0.004079936148999268	0.0
28	2.5499600931245425E-4	0.0	0.041564349517930047	0.014789768540122348	0.0
29	2.5499600931245425E-4	0.0	0.041564349517930047	0.039269385434117954	0.0
30	2.5499600931245425E-4	0.0	0.041564349517930047	0.06425899434673847	0.0
31	2.5499600931245425E-4	0.0	0.041564349517930047	0.14789768540122347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	275	0.0	28.927273	1
TCTATAC	80	9.731593E-10	25.4375	3
GTATCAA	855	0.0	25.099415	1
ATACCGC	40	0.0019289315	23.125	6
CCGCTCT	105	9.767973E-10	21.142859	28
GCCGCTC	105	9.767973E-10	21.142859	27
CGCTCTC	105	9.767973E-10	21.142859	29
GCGGTAA	45	0.003821382	20.555555	23
CGGTTCG	45	0.003821382	20.555555	22
CGTATTA	100	1.283297E-8	20.349998	2
TCTAATA	100	1.283297E-8	20.349998	2
TTAGCTC	55	5.134794E-4	20.181818	4
GTCTACG	55	5.134794E-4	20.181818	1
AACTCCG	55	5.134794E-4	20.181818	5
CGGAGTT	110	1.7444108E-9	20.181818	24
AGCTTCG	120	2.3646862E-10	20.041666	21
CGCGATA	65	6.887321E-5	19.923077	34
GCTTCGC	130	3.274181E-11	19.923077	22
TTCGCCG	125	4.0927262E-10	19.240002	24
CGGCAGC	140	9.276846E-11	18.5	17
>>END_MODULE