FastQCFastQC Report
Fri 10 Feb 2017
ERR1632820.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632820.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321267
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8620.2683126496029782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.17524364469428855No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4700.14629576022436167No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3780.11765914332938024No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC3750.11672534060454388No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3440.10707604578123492No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC3260.10147322943221682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA200.001839962937.029
GACGTGT250.005490522429.636
GGTATCA1900.027.2631591
CCGTGCC509.06449E-625.9000029
ACGATGT400.001928176123.12522
CGCCGCT400.001928176123.12526
ATACCCT502.6962333E-422.24
AGGAGCG450.003819899620.5555557
CCGCTCT450.003819899620.55555528
GCCGCTC450.003819899620.55555527
CGATGTC450.003819899620.55555523
GTACGAT450.003819899620.55555520
GTGTTAT555.1320164E-420.1818181
GCACCTC555.1320164E-420.1818186
GTATCAA4450.019.1235961
TAGAGAT609.2170824E-418.54
AGCTTCG500.007024121518.521
CGCTCTC500.007024121518.529
ACTAGAA1205.133188E-918.528
TACGATG500.007024121518.521
Produced by FastQC (version 0.11.5)