##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632820.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321267
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.720836562734423	33.0	33.0	33.0	27.0	33.0
2	31.239483046811532	33.0	33.0	33.0	27.0	33.0
3	31.67291069422007	33.0	33.0	33.0	27.0	33.0
4	31.819137975577945	33.0	33.0	33.0	27.0	33.0
5	31.916026233631218	33.0	33.0	33.0	33.0	33.0
6	34.78390871144562	37.0	37.0	37.0	27.0	37.0
7	34.98271842423903	37.0	37.0	37.0	33.0	37.0
8	35.13789153570083	37.0	37.0	37.0	33.0	37.0
9	35.212141303028325	37.0	37.0	37.0	33.0	37.0
10	35.259643847640746	37.0	37.0	37.0	33.0	37.0
11	35.29369029498828	37.0	37.0	37.0	33.0	37.0
12	35.24500804626681	37.0	37.0	37.0	33.0	37.0
13	35.273464127968325	37.0	37.0	37.0	33.0	37.0
14	35.23694932875147	37.0	37.0	37.0	33.0	37.0
15	35.251939975160845	37.0	37.0	37.0	33.0	37.0
16	35.26468326967911	37.0	37.0	37.0	33.0	37.0
17	35.221871527421115	37.0	37.0	37.0	33.0	37.0
18	35.24794952485005	37.0	37.0	37.0	33.0	37.0
19	35.243682046397545	37.0	37.0	37.0	33.0	37.0
20	35.23711741324195	37.0	37.0	37.0	33.0	37.0
21	35.22927347035332	37.0	37.0	37.0	33.0	37.0
22	35.2233936258626	37.0	37.0	37.0	33.0	37.0
23	35.245185468784534	37.0	37.0	37.0	33.0	37.0
24	35.2236768793558	37.0	37.0	37.0	33.0	37.0
25	35.22759885080011	37.0	37.0	37.0	33.0	37.0
26	35.17564206719022	37.0	37.0	37.0	33.0	37.0
27	35.120192861389434	37.0	37.0	37.0	33.0	37.0
28	35.11562034071349	37.0	37.0	37.0	33.0	37.0
29	35.15670143525479	37.0	37.0	37.0	33.0	37.0
30	35.12146905844671	37.0	37.0	37.0	33.0	37.0
31	35.0932900048869	37.0	37.0	37.0	33.0	37.0
32	35.12538480453953	37.0	37.0	37.0	33.0	37.0
33	35.09834810297976	37.0	37.0	37.0	33.0	37.0
34	35.03931932006711	37.0	37.0	37.0	27.0	37.0
35	35.03621286966916	37.0	37.0	37.0	27.0	37.0
36	35.029533067510826	37.0	37.0	37.0	27.0	37.0
37	35.03358888401236	37.0	37.0	37.0	27.0	37.0
38	34.903740502448116	37.0	37.0	37.0	27.0	37.0
39	34.9945932822232	37.0	37.0	37.0	27.0	37.0
40	35.01847995592451	37.0	37.0	37.0	27.0	37.0
41	35.02360030753236	37.0	37.0	37.0	27.0	37.0
42	34.9438068646951	37.0	37.0	37.0	27.0	37.0
43	34.72959563229339	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	5.0
16	11.0
17	14.0
18	10.0
19	11.0
20	15.0
21	64.0
22	191.0
23	415.0
24	833.0
25	1511.0
26	2607.0
27	3876.0
28	5640.0
29	7629.0
30	9896.0
31	12646.0
32	16379.0
33	21202.0
34	30111.0
35	54684.0
36	153516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.97422704479452	21.140982422720043	13.77763666981047	25.10715386267497
2	15.787802668808187	23.012011815717145	38.0325399122848	23.16764560318987
3	17.113180002925915	27.634957838806972	30.02487027923814	25.226991879028972
4	12.367283287732633	18.178648911964192	39.97733972054397	29.4767280797592
5	13.361471922108404	37.42058785994204	36.24586403209791	12.972076185851645
6	29.01729713913972	40.50649459795124	17.679686989326633	12.796521273582409
7	26.323587545561793	31.894654601935464	24.736122913339994	17.045634939162753
8	23.722946956892553	36.53160766589784	20.895081038513137	18.850364338696473
9	25.46853551718664	15.739244927116697	20.559223325147	38.23299623054967
10	14.606542221890203	28.2179620066798	34.815589525223565	22.359906246206425
11	33.395586848322424	24.14315818306891	24.604145461563125	17.85710950704554
12	22.332825967186174	26.350045289432156	30.43947868906548	20.87765005431619
13	27.333962093834725	22.48877102223384	26.28063262021932	23.896634263712116
14	21.730523209666725	20.990951451596334	27.82763246769821	29.450892871038732
15	23.449965293665393	29.265377396371246	24.47092293948647	22.813734370476894
16	22.87567661789104	28.646577457379685	25.57530029539293	22.902445629336345
17	22.130190775896686	28.07571272492973	26.445293167365463	23.34880333180812
18	22.62790762823446	27.97112681974806	27.653322625728755	21.747642926288727
19	24.006200450092912	26.637656528681752	27.543133904198065	21.81300911702727
20	23.367790653879794	27.076543809354835	27.60756629221177	21.948099244553596
21	22.438034407517733	27.652700090578865	27.545935312372578	22.363330189530824
22	22.266214706147846	27.34018744533363	27.470608559235778	22.922989289282746
23	22.37266821677919	27.256456467673306	27.982021184871154	22.388854130676354
24	22.536083693625553	28.03618174291166	26.160483336290376	23.267251227172416
25	22.917075205358785	27.36633392162905	27.64803107695468	22.068559796057485
26	22.626973825509623	27.571770521093047	27.610678967961228	22.1905766854361
27	22.374535822228864	27.661726850252283	26.789243837680182	23.17449348983867
28	22.408463987897917	27.14191000009338	28.22979017452773	22.219835837480975
29	23.637348373782554	26.957639595725674	27.074676203905163	22.33033582658661
30	23.030687870213875	26.9112607270588	27.382208567951267	22.67584283477606
31	22.81093296230238	26.998104380468586	27.677912764149447	22.513049893079586
32	22.181549925762685	26.938963541228944	27.711529662243557	23.167956870764815
33	21.9207076979584	27.27824519791949	27.970815552173118	22.830231551949
34	22.92547942988231	26.609019911786767	27.881481758163773	22.58401890016715
35	22.63880199335755	27.545001509647737	27.377850821902033	22.43834567509268
36	23.58816809694117	27.18299731998618	26.68030018644928	22.548534396623367
37	22.708214662570388	26.611821319961276	27.366022654054106	23.31394136341423
38	22.76673296666013	27.346412796832542	27.151248027341744	22.735606209165585
39	22.17096682821454	27.189533939060034	27.901091615385333	22.738407617340094
40	22.498420317057153	27.359797302555194	28.091898638826894	22.04988374156076
41	22.378582300703155	26.51377203385346	27.73860994126381	23.369035724179575
42	22.126766832572283	27.675422623549885	27.43823673144145	22.759573812436386
43	23.00329632361867	26.41261007199619	27.62063953035948	22.963454074025655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	9.0
2	12.0
3	54.5
4	97.0
5	97.0
6	114.0
7	131.0
8	133.0
9	135.0
10	175.0
11	215.0
12	215.0
13	324.0
14	433.0
15	769.5
16	1106.0
17	1341.0
18	1576.0
19	1576.0
20	1540.0
21	1504.0
22	1836.0
23	2168.0
24	2972.5
25	3777.0
26	3777.0
27	5169.0
28	6561.0
29	8025.0
30	9489.0
31	11513.5
32	13538.0
33	13538.0
34	15768.5
35	17999.0
36	20039.0
37	22079.0
38	23972.0
39	25865.0
40	25865.0
41	27037.5
42	28210.0
43	28711.0
44	29212.0
45	28385.0
46	27558.0
47	27558.0
48	25932.5
49	24307.0
50	23496.0
51	22685.0
52	21086.0
53	19487.0
54	19487.0
55	18626.5
56	17766.0
57	15068.0
58	12370.0
59	11570.0
60	10770.0
61	10770.0
62	9382.0
63	7994.0
64	6583.0
65	5172.0
66	4311.5
67	3451.0
68	3451.0
69	2837.0
70	2223.0
71	1803.5
72	1384.0
73	1142.5
74	901.0
75	901.0
76	690.5
77	480.0
78	368.0
79	256.0
80	197.0
81	138.0
82	138.0
83	115.5
84	93.0
85	76.0
86	59.0
87	48.0
88	37.0
89	37.0
90	28.5
91	20.0
92	10.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	321267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.97876247443639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04877802442613	56.081818398391434
2	5.71054217894641	7.192857589762326
3	1.650064566690232	3.1175707323920374
4	0.7759595538195455	1.9547588971908318
5	0.541335910744074	1.7046332870816865
6	0.33993105009211755	1.2845062116862327
7	0.25464278133247575	1.1225961068957486
8	0.18025229037241136	0.9081652944669786
9	0.16713713406001596	0.9473480879961398
>10	1.1494244798720616	14.106406668018229
>50	0.1290969744600768	5.51797696011355
>100	0.05183816735076432	5.61403458611165
>500	9.968878336685447E-4	0.44732717989315035
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	862	0.2683126496029782	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	563	0.17524364469428855	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	470	0.14629576022436167	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	378	0.11765914332938024	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	375	0.11672534060454388	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	344	0.10707604578123492	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	326	0.10147322943221682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	3.1126757494545035E-4	0.0	0.0
4	0.0	0.0	3.1126757494545035E-4	0.0	0.0
5	0.0	0.0	3.1126757494545035E-4	0.0	0.0
6	0.0	0.0	0.0018676054496727021	0.0	0.0
7	0.0	0.0	0.003423943324399954	0.0	0.0
8	0.0	0.0	0.004046478474290855	0.0	0.0
9	0.0	0.0	0.004357746049236305	0.0	0.0
10	0.0	0.0	0.004357746049236305	0.0	0.0
11	0.0	0.0	0.006536619073854458	0.0	0.0
12	0.0	0.0	0.007781689373636259	0.0	0.0
13	0.0	0.0	0.00902675967341806	0.0	0.0
14	0.0	0.0	0.009649294823308962	0.0	0.0
15	0.0	0.0	0.009960562398254411	0.0	0.0
16	0.0	0.0	0.010894365123090763	0.0	0.0
17	0.0	0.0	0.010894365123090763	0.0	0.0
18	0.0	0.0	0.010894365123090763	0.0	0.0
19	0.0	0.0	0.010894365123090763	0.0	0.0
20	0.0	0.0	0.010894365123090763	0.0	0.0
21	0.0	0.0	0.011516900272981663	0.0	0.0
22	0.0	0.0	0.011516900272981663	6.225351498909007E-4	0.0
23	0.0	0.0	0.012139435422872564	6.225351498909007E-4	0.0
24	0.0	0.0	0.012139435422872564	9.338027248363511E-4	0.0
25	0.0	0.0	0.012139435422872564	9.338027248363511E-4	0.0
26	0.0	0.0	0.012139435422872564	9.338027248363511E-4	0.0
27	0.0	0.0	0.012139435422872564	0.0015563378747272519	0.0
28	0.0	0.0	0.012139435422872564	0.0074704217986908085	0.0
29	0.0	0.0	0.012139435422872564	0.018364786921781572	0.0
30	0.0	0.0	0.012139435422872564	0.039530982018072196	0.0
31	0.0	0.0	0.012139435422872564	0.09493661035836236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCCA	20	0.0018399629	37.0	29
GACGTGT	25	0.0054905224	29.6	36
GGTATCA	190	0.0	27.263159	1
CCGTGCC	50	9.06449E-6	25.900002	9
ACGATGT	40	0.0019281761	23.125	22
CGCCGCT	40	0.0019281761	23.125	26
ATACCCT	50	2.6962333E-4	22.2	4
AGGAGCG	45	0.0038198996	20.555555	7
CCGCTCT	45	0.0038198996	20.555555	28
GCCGCTC	45	0.0038198996	20.555555	27
CGATGTC	45	0.0038198996	20.555555	23
GTACGAT	45	0.0038198996	20.555555	20
GTGTTAT	55	5.1320164E-4	20.181818	1
GCACCTC	55	5.1320164E-4	20.181818	6
GTATCAA	445	0.0	19.123596	1
TAGAGAT	60	9.2170824E-4	18.5	4
AGCTTCG	50	0.0070241215	18.5	21
CGCTCTC	50	0.0070241215	18.5	29
ACTAGAA	120	5.133188E-9	18.5	28
TACGATG	50	0.0070241215	18.5	21
>>END_MODULE