FastQCFastQC Report
Fri 10 Feb 2017
ERR1632819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218673
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59692.7296465498712688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49792.2769157600618275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23951.095242668276376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.5794039501904671No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG11910.5446488592556008No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9590.43855437113864076No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9450.432152117545376No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9210.42117682567120773No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8710.3983116342666905No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG7430.3397767442711263No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.3256003256003256No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6970.31874076817897046No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6710.30685086864862143No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6390.29221714614973043No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA6110.2794126389632008No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5650.2583766628710449No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5330.24374294037215385No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5230.2391699020912504No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC5110.23368225615416627No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4740.2167620145148235No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4720.21584740685864282No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4580.209445153265378No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4500.2057867226406552No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4440.20304289967211317No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4440.20304289967211317No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4170.19069569631367383No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4120.18840917717322211No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.1852080503765897No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4040.18475074654849935No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4030.184293442720409No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3990.18246422740804763No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3990.18246422740804763No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3960.18109231592377661No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3790.17331815084624072No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3770.17240354319006004No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3760.17194623936196968No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3730.17057432787769866No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3720.17011702404960832No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.16828780873724694No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3680.16828780873724694No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3630.16600128959679522No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3560.1628001628001628No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3560.1628001628001628No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC3550.16234285897207246No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3420.15639790920689797No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3360.15365408623835589No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3330.15228217475408487No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3290.1504529594417235No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3280.14999565561363315No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3220.14725183264509106No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3210.14679452881700072No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3160.144508009676549No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3160.144508009676549No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3150.14405070584845864No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3050.1394776675675552No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3050.1394776675675552No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA3000.1371911484271035No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2980.1362765407709228No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2930.13399002163047108No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG2860.13078889483383865No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA2840.12987428717765795No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2810.12850237569338693No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2770.12667316038102555No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2770.12667316038102555No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2760.1262158565529352No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG2740.12530124889675454No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG2690.12301472975630279No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2690.12301472975630279No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA2670.1221001221001221No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2620.11981360295967038No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2610.11935629913158002No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2580.117984387647309No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2570.11752708381921864No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2560.1170697799911283No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2530.11569786850685727No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.11569786850685727No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2520.11524056467876692No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG2500.11432595702258624No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2480.11341134936640555No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC2480.11341134936640555No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2470.1129540455383152No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA2440.11158213405404417No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2440.11158213405404417No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC2410.11021022256977313No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.10929561491359245No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2390.10929561491359245No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2380.1088383110855021No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA2300.10517988046077935No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2280.10426527280459864No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2280.10426527280459864No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG2270.1038079689765083No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2250.1028933613203276No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2240.10243605749223728No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2230.10197875366414692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA401.5922342E-632.3751
GTGACAT453.9887727E-628.77777716
GCCGCTC3100.028.6451627
CGCTCTC3050.028.50819629
CCGCTCT3200.028.32812528
TTCGCCG3200.027.7524
GGCAGCT3150.027.60317418
GCTCTCT3150.027.60317430
GCAGCTT3200.027.17187519
GGTATCA7200.026.9791661
ATATTCT358.84635E-426.428574
CGGCAGC3250.026.18461417
GCCGGCA3250.026.18461415
CTCTCTT3250.026.18461431
CCGGCAG3350.025.95522316
CAGCTTC3350.025.95522320
TGACATC509.0472E-625.90000217
TATTATA509.0472E-625.9000022
TCTCTTC3300.025.78787832
GCTTCGC3400.025.57352822
Produced by FastQC (version 0.11.5)