FastQCFastQC Report
Fri 10 Feb 2017
ERR1632818.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632818.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235251
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT7220.30690624056858423No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6850.2911783584341831No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC6510.27672570998635504No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG6380.27119969734453836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5560.2363433099115413No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5310.22571636252343244No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5290.22486620673238372No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5130.21806496040399403No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA4820.204887545642739No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT4330.18405872876204565No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA4230.1798079498068021No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC4110.17470701506050984No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4070.17300670347841243No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC4060.17258162558288806No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG3790.16110452240373047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.16067944450820612No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT3590.15260296449324337No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA3530.15005249712009724No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG3430.1458017181648537No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC3350.14240109500065887No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG3240.137725238149891No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG3140.13347445919464743No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA3110.13219922550807436No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA3110.13219922550807436No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC2980.12667321286625774No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT2950.12539797917968468No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC2930.12454782338863597No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC2830.12029704443339242No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA2790.11859673285129499No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA2780.11817165495577064No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT2740.11647134337367322No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA2740.11647134337367322No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA2700.11477103179157581No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA2630.11179548652290533No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG2610.1109453307318566No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA2530.10754470756766177No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTATTTTGTGGCT2530.10754470756766177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAACA200.001838962237.025
CGGCAAA250.00548757129.634
TCGGCAA250.00548757129.633
CTCGGCA250.00548757129.632
GGTATCA905.456968E-1226.7222211
TTGTACC509.051018E-625.9000024
GTATCAA3250.025.0461561
TCGCCAT701.9094296E-723.78571513
TGTACCA551.8943321E-523.5454545
ACTTAGA400.001926649523.1251
TTATACT400.001926649523.1254
ACCGTAC400.001926649523.12527
GCCTTGT502.6932594E-422.21
CATGCTA502.6932594E-422.24
CCTATAC502.6932594E-422.23
CGGCAAG1301.8189894E-1221.34615318
GCCATTG806.911814E-720.812515
GGGTGAT450.00381690520.5555557
ATCAACG4050.020.5555553
TCAACGC4050.020.5555554
Produced by FastQC (version 0.11.5)