##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632818.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	235251
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.340891218315758	33.0	33.0	33.0	27.0	33.0
2	30.967672826045373	33.0	33.0	33.0	27.0	33.0
3	31.413439262744898	33.0	33.0	33.0	27.0	33.0
4	31.559470522973335	33.0	33.0	33.0	27.0	33.0
5	31.675380763524917	33.0	33.0	33.0	27.0	33.0
6	34.461077742496315	37.0	33.0	37.0	27.0	37.0
7	34.66779737386876	37.0	37.0	37.0	27.0	37.0
8	34.80089351373639	37.0	37.0	37.0	27.0	37.0
9	34.88769016922351	37.0	37.0	37.0	27.0	37.0
10	34.93024896812341	37.0	37.0	37.0	27.0	37.0
11	34.974010737467644	37.0	37.0	37.0	27.0	37.0
12	34.92580265333623	37.0	37.0	37.0	27.0	37.0
13	34.96602777458969	37.0	37.0	37.0	27.0	37.0
14	34.94477813059243	37.0	37.0	37.0	27.0	37.0
15	34.96138592397057	37.0	37.0	37.0	27.0	37.0
16	34.94111820991196	37.0	37.0	37.0	27.0	37.0
17	34.89443615542548	37.0	37.0	37.0	27.0	37.0
18	34.903787869127015	37.0	37.0	37.0	27.0	37.0
19	34.91907792102903	37.0	37.0	37.0	27.0	37.0
20	34.95434663402069	37.0	37.0	37.0	27.0	37.0
21	34.90845097364092	37.0	37.0	37.0	27.0	37.0
22	34.93289295263357	37.0	37.0	37.0	27.0	37.0
23	34.92642326706369	37.0	37.0	37.0	27.0	37.0
24	34.915426501906474	37.0	37.0	37.0	27.0	37.0
25	34.88832353528784	37.0	37.0	37.0	27.0	37.0
26	34.821922117227984	37.0	37.0	37.0	27.0	37.0
27	34.77325919974835	37.0	37.0	37.0	27.0	37.0
28	34.75109988905467	37.0	37.0	37.0	27.0	37.0
29	34.788192186218126	37.0	37.0	37.0	27.0	37.0
30	34.75749731138231	37.0	37.0	37.0	27.0	37.0
31	34.75149946227646	37.0	37.0	37.0	27.0	37.0
32	34.78433672970572	37.0	37.0	37.0	27.0	37.0
33	34.757433549697986	37.0	37.0	37.0	27.0	37.0
34	34.68224152075868	37.0	37.0	37.0	27.0	37.0
35	34.67872187578374	37.0	37.0	37.0	27.0	37.0
36	34.64507696035299	37.0	37.0	37.0	27.0	37.0
37	34.69637536078486	37.0	37.0	37.0	27.0	37.0
38	34.5315981653638	37.0	37.0	37.0	27.0	37.0
39	34.61944051247391	37.0	37.0	37.0	27.0	37.0
40	34.65142762411212	37.0	37.0	37.0	27.0	37.0
41	34.654245890559444	37.0	37.0	37.0	27.0	37.0
42	34.60022274081725	37.0	37.0	37.0	27.0	37.0
43	34.30872982474038	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	15.0
17	11.0
18	10.0
19	9.0
20	29.0
21	77.0
22	199.0
23	481.0
24	994.0
25	1597.0
26	2631.0
27	3683.0
28	5185.0
29	6894.0
30	8556.0
31	10679.0
32	13355.0
33	17239.0
34	23788.0
35	41928.0
36	97887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.62278587551169	21.612235442144772	14.001640800676723	24.763337881666818
2	15.133198158562555	24.316581013470717	38.44234455964056	22.10787626832617
3	17.49408079030482	27.23559092203646	32.2523602450149	23.017968042643812
4	13.176989683359475	18.94699703720707	38.314396113087724	29.561617166345734
5	13.260304950882249	38.479326336551175	35.756702415717676	12.503666296848898
6	28.94440406204437	39.85147778330379	18.063260092411934	13.140858062239905
7	24.564826504456942	33.54799767057313	24.50531559908353	17.381860225886395
8	22.899796387688042	37.84851073959303	21.320206927919543	17.931485944799384
9	24.7072275994576	16.726390110987836	20.40926499781085	38.15711729174372
10	13.821407772974398	29.651308602301373	34.78539942444453	21.741884200279703
11	31.949704783401558	24.574603296054	25.577362051595955	17.898329868948483
12	21.255170009904315	26.68086426837718	30.740358170634767	21.323607551083736
13	26.315297278226236	22.815205886478697	28.17628830483186	22.693208530463206
14	20.93933713352972	22.23837518225215	27.786491874636027	29.035795809582105
15	23.06217614377835	29.727822623495754	25.21902138566892	21.990979847056973
16	22.344219578237713	30.05640783673608	24.937194740936278	22.66217784408993
17	21.957823771206073	28.931226647283115	26.623478752481393	22.48747082902942
18	20.749752392125856	29.06937696332853	28.21752086069772	21.963349783847892
19	23.457073508720473	28.005406990831073	28.497647193848273	20.039872306600184
20	23.17779733136097	27.046856336423648	29.006465434790922	20.768880897424452
21	21.29257686471046	28.94525421783542	27.13569761658824	22.626471300865884
22	21.233491037232575	28.64174860043103	27.455356194022556	22.669404168313847
23	21.234766270919145	28.975009670522123	27.642390468053268	22.14783359050546
24	23.210528329316347	28.761620566968897	26.646007880944182	21.381843222770573
25	22.858988909717706	27.54674794156029	27.89403658220369	21.700226566518317
26	22.255803375968647	27.72060480082975	27.396270366544666	22.627321456656933
27	22.007132807086897	27.460032050873323	27.57395292687385	22.95888221516593
28	20.693642109916645	28.529953113908125	28.42028301686284	22.356121759312394
29	22.56100930495513	28.81305499232734	27.138248083961386	21.487687618756137
30	21.343161134277857	29.1055085844481	27.3410102401265	22.21032004114754
31	22.474293414268164	28.125704035264466	27.7197546450387	21.680247905428672
32	21.452406153427617	28.264279429205402	27.83367552103923	22.449638896327752
33	21.829450246757716	27.709552775546122	27.967575058129402	22.49342191956676
34	21.168454119217348	28.217095782802197	27.971825837084648	22.642624260895808
35	22.237099948565575	28.532503581281272	27.19648375564822	22.033912714504932
36	22.20351879481915	28.80072773335714	26.740800251646114	22.254953220177597
37	23.06557676694254	27.116994189185167	27.131446837632993	22.685982206239295
38	22.931252151956848	28.068743597264202	27.242392168364855	21.757612082414102
39	22.079821127221564	27.92931804753221	27.993929887651912	21.996930937594314
40	22.416907898372376	27.325707435887626	27.806045457830148	22.451339207909847
41	21.652192764324063	28.03983830036854	28.16396104586165	22.144007889445742
42	22.630722079821126	27.28022410106652	27.600732834291886	22.48832098482047
43	21.96122439437027	27.460457128768844	27.87660838848719	22.701710088373694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	8.0
2	8.0
3	37.0
4	66.0
5	66.0
6	80.0
7	94.0
8	92.0
9	90.0
10	135.0
11	180.0
12	180.0
13	248.5
14	317.0
15	741.5
16	1166.0
17	1364.0
18	1562.0
19	1562.0
20	1608.5
21	1655.0
22	2254.0
23	2853.0
24	3465.5
25	4078.0
26	4078.0
27	5055.0
28	6032.0
29	8250.5
30	10469.0
31	10772.0
32	11075.0
33	11075.0
34	12122.5
35	13170.0
36	14186.5
37	15203.0
38	16776.5
39	18350.0
40	18350.0
41	19669.0
42	20988.0
43	19148.5
44	17309.0
45	17626.0
46	17943.0
47	17943.0
48	17691.0
49	17439.0
50	17942.0
51	18445.0
52	18615.5
53	18786.0
54	18786.0
55	14940.0
56	11094.0
57	9658.5
58	8223.0
59	7067.0
60	5911.0
61	5911.0
62	5127.5
63	4344.0
64	3612.5
65	2881.0
66	2434.5
67	1988.0
68	1988.0
69	1677.0
70	1366.0
71	1117.0
72	868.0
73	716.0
74	564.0
75	564.0
76	432.5
77	301.0
78	246.0
79	191.0
80	144.0
81	97.0
82	97.0
83	78.0
84	59.0
85	51.5
86	44.0
87	34.0
88	24.0
89	24.0
90	17.0
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	235251.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.39795869323667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.89021667194994	43.79089550679316
2	6.722991243922355	6.776500700123813
3	1.8598010779053646	2.8119053370573477
4	0.9539440916417983	1.9230733970488207
5	0.5975003985654507	1.5056400203047013
6	0.42856003269357323	1.2959130495157714
7	0.3071161032572288	1.0834617280189935
8	0.2514253737774897	1.013706048165561
9	0.17787929870964816	0.8068278193875678
>10	1.4160573529669276	14.456073549672388
>50	0.23352509655769435	8.383668509288961
>100	0.15303346753142524	13.735894635858326
>500	0.007949790521112998	2.41643969876459
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	722	0.30690624056858423	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	685	0.2911783584341831	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	651	0.27672570998635504	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	638	0.27119969734453836	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.2363433099115413	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	531	0.22571636252343244	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	529	0.22486620673238372	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	513	0.21806496040399403	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	482	0.204887545642739	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	433	0.18405872876204565	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	423	0.1798079498068021	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	411	0.17470701506050984	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	407	0.17300670347841243	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	406	0.17258162558288806	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	379	0.16110452240373047	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.16067944450820612	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	359	0.15260296449324337	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	353	0.15005249712009724	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	343	0.1458017181648537	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	335	0.14240109500065887	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	324	0.137725238149891	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	314	0.13347445919464743	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	311	0.13219922550807436	No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA	311	0.13219922550807436	No Hit
CTGTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGC	298	0.12667321286625774	No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT	295	0.12539797917968468	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	293	0.12454782338863597	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	283	0.12029704443339242	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	279	0.11859673285129499	No Hit
ATGCAAAGCAATGTGGCCTCAGAATACACCTCTTAAATTTACA	278	0.11817165495577064	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	274	0.11647134337367322	No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA	274	0.11647134337367322	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	270	0.11477103179157581	No Hit
GGAAATAACTATATCACTATTCAAGATCATCTTCACAACATCA	263	0.11179548652290533	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	261	0.1109453307318566	No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA	253	0.10754470756766177	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTATTTTGTGGCT	253	0.10754470756766177	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.2507789552435486E-4	0.0	0.0
2	0.0	0.0	4.2507789552435486E-4	0.0	0.0
3	0.0	0.0	4.2507789552435486E-4	0.0	0.0
4	0.0	0.0	4.2507789552435486E-4	0.0	0.0
5	0.0	0.0	8.501557910487097E-4	0.0	0.0
6	0.0	0.0	0.0012752336865730644	0.0	0.0
7	0.0	0.0	0.0017003115820974195	0.0	0.0
8	0.0	0.0	0.0017003115820974195	0.0	0.0
9	0.0	0.0	0.0021253894776217743	4.2507789552435486E-4	0.0
10	0.0	0.0	0.002550467373146129	4.2507789552435486E-4	0.0
11	0.0	0.0	0.0038257010597191935	4.2507789552435486E-4	0.0
12	0.0	0.0	0.0038257010597191935	4.2507789552435486E-4	0.0
13	0.0	0.0	0.0042507789552435485	4.2507789552435486E-4	0.0
14	0.0	0.0	0.004675856850767903	4.2507789552435486E-4	0.0
15	0.0	0.0	0.005526012641816613	8.501557910487097E-4	0.0
16	0.0	0.0	0.007226324223914032	8.501557910487097E-4	0.0
17	0.0	0.0	0.008076480014962742	8.501557910487097E-4	0.0
18	0.0	0.0	0.008501557910487097	8.501557910487097E-4	0.0
19	0.0	0.0	0.00892663580601145	8.501557910487097E-4	0.0
20	0.0	0.0	0.009776791597060162	8.501557910487097E-4	0.0
21	0.0	0.0	0.009776791597060162	8.501557910487097E-4	0.0
22	0.0	0.0	0.010201869492584515	0.0012752336865730644	0.0
23	0.0	0.0	0.010201869492584515	0.0012752336865730644	0.0
24	0.0	0.0	0.010201869492584515	0.0012752336865730644	0.0
25	0.0	0.0	0.010201869492584515	0.0012752336865730644	0.0
26	0.0	0.0	0.010201869492584515	0.0012752336865730644	0.0
27	0.0	0.0	0.010201869492584515	0.0021253894776217743	0.0
28	0.0	0.0	0.010201869492584515	0.00892663580601145	0.0
29	0.0	0.0	0.010201869492584515	0.017003115820974194	0.0
30	0.0	0.0	0.010201869492584515	0.03400623164194839	0.0
31	0.0	0.0	0.010201869492584515	0.0930920591198337	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGAACA	20	0.0018389622	37.0	25
CGGCAAA	25	0.005487571	29.6	34
TCGGCAA	25	0.005487571	29.6	33
CTCGGCA	25	0.005487571	29.6	32
GGTATCA	90	5.456968E-12	26.722221	1
TTGTACC	50	9.051018E-6	25.900002	4
GTATCAA	325	0.0	25.046156	1
TCGCCAT	70	1.9094296E-7	23.785715	13
TGTACCA	55	1.8943321E-5	23.545454	5
ACTTAGA	40	0.0019266495	23.125	1
TTATACT	40	0.0019266495	23.125	4
ACCGTAC	40	0.0019266495	23.125	27
GCCTTGT	50	2.6932594E-4	22.2	1
CATGCTA	50	2.6932594E-4	22.2	4
CCTATAC	50	2.6932594E-4	22.2	3
CGGCAAG	130	1.8189894E-12	21.346153	18
GCCATTG	80	6.911814E-7	20.8125	15
GGGTGAT	45	0.003816905	20.555555	7
ATCAACG	405	0.0	20.555555	3
TCAACGC	405	0.0	20.555555	4
>>END_MODULE