FastQCFastQC Report
Fri 10 Feb 2017
ERR1632817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412490
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12850.31152270358069284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.21818710756624404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4850.11757860796625373No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4270.10351766103420688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATT451.3212037E-424.66666622
ACGTAGA451.3212037E-424.66666624
GTATCAA6800.024.2132341
GTCCTAG502.698034E-422.1999991
CTGCTCG1450.021.6896559
CGCTCTC603.7188343E-521.58333429
TGCTCGG957.1122486E-921.42105310
GCTCGGA957.1122486E-921.42105311
GGTATCA2800.020.4821431
ACCGCAA1001.2838427E-820.3499988
TTAACGG1001.2838427E-820.34999835
CCTACGT555.1354134E-420.18181834
CTACGTG555.1354134E-420.18181835
TACGTGA555.1354134E-420.18181836
CTCGGAG1202.3646862E-1020.04166812
TTCGCCG656.888425E-519.92307724
ACAATGC759.242169E-619.7333348
TAACGGC1052.2489985E-819.3809536
AACGGCC1052.2489985E-819.3809537
GGTCGCC1355.4569682E-1119.18518636
Produced by FastQC (version 0.11.5)