##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412490
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.753986763315474	33.0	33.0	33.0	27.0	33.0
2	31.26326456398943	33.0	33.0	33.0	27.0	33.0
3	31.70791776770346	33.0	33.0	33.0	27.0	33.0
4	31.843472569031977	33.0	33.0	33.0	27.0	33.0
5	31.938223956944412	33.0	33.0	33.0	33.0	33.0
6	34.83864578535237	37.0	37.0	37.0	27.0	37.0
7	34.98114620960508	37.0	37.0	37.0	33.0	37.0
8	35.18618390748867	37.0	37.0	37.0	33.0	37.0
9	35.243908943247106	37.0	37.0	37.0	33.0	37.0
10	35.28997551455793	37.0	37.0	37.0	33.0	37.0
11	35.33665785837233	37.0	37.0	37.0	33.0	37.0
12	35.24581686828772	37.0	37.0	37.0	33.0	37.0
13	35.31975320613833	37.0	37.0	37.0	33.0	37.0
14	35.25559407500788	37.0	37.0	37.0	33.0	37.0
15	35.285788746393855	37.0	37.0	37.0	33.0	37.0
16	35.29106645009576	37.0	37.0	37.0	33.0	37.0
17	35.27304419501079	37.0	37.0	37.0	33.0	37.0
18	35.25006666828287	37.0	37.0	37.0	33.0	37.0
19	35.285252975829714	37.0	37.0	37.0	33.0	37.0
20	35.21855075274552	37.0	37.0	37.0	33.0	37.0
21	35.282986254212226	37.0	37.0	37.0	33.0	37.0
22	35.2878784940241	37.0	37.0	37.0	33.0	37.0
23	35.26409609929938	37.0	37.0	37.0	33.0	37.0
24	35.25676016388276	37.0	37.0	37.0	33.0	37.0
25	35.26669495018061	37.0	37.0	37.0	33.0	37.0
26	35.22567819825935	37.0	37.0	37.0	33.0	37.0
27	35.15720381100148	37.0	37.0	37.0	33.0	37.0
28	35.15911900894567	37.0	37.0	37.0	33.0	37.0
29	35.18471235666319	37.0	37.0	37.0	33.0	37.0
30	35.165924022400546	37.0	37.0	37.0	33.0	37.0
31	35.14727629760721	37.0	37.0	37.0	33.0	37.0
32	35.187643336808165	37.0	37.0	37.0	33.0	37.0
33	35.1589541564644	37.0	37.0	37.0	33.0	37.0
34	35.11058449901816	37.0	37.0	37.0	33.0	37.0
35	35.09846541734345	37.0	37.0	37.0	33.0	37.0
36	35.082787461514215	37.0	37.0	37.0	33.0	37.0
37	35.106846226575186	37.0	37.0	37.0	33.0	37.0
38	34.95470920507164	37.0	37.0	37.0	27.0	37.0
39	35.020577468544694	37.0	37.0	37.0	27.0	37.0
40	35.064377318238016	37.0	37.0	37.0	27.0	37.0
41	35.08695968387112	37.0	37.0	37.0	33.0	37.0
42	34.95295643530752	37.0	37.0	37.0	27.0	37.0
43	34.786894227738856	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	10.0
16	18.0
17	15.0
18	13.0
19	17.0
20	28.0
21	66.0
22	207.0
23	458.0
24	1025.0
25	1954.0
26	3184.0
27	4835.0
28	7004.0
29	9285.0
30	12272.0
31	15945.0
32	20501.0
33	27099.0
34	38838.0
35	70525.0
36	199187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47079929210405	20.890445828989794	13.570753230381344	25.068001648524813
2	16.082814128827366	23.228926761860894	37.62466968896216	23.063589420349583
3	17.522849038764576	26.612523939974302	30.804383136560887	25.060243884700235
4	12.729763145773232	18.198501781861378	39.10591771921744	29.965817353147955
5	13.974156949259376	36.93253169773813	35.442556183180194	13.650755169822299
6	29.42398603602512	40.03151591553735	17.571819922907224	12.972678125530315
7	26.22390845838687	32.43569541079784	23.641057965041576	17.699338165773714
8	24.29780115881597	35.71092632548668	20.947416907076537	19.043855608620817
9	25.911658464447623	15.22509636597251	20.248975732745038	38.61426943683483
10	14.813934883269898	28.128924337559695	34.11646342941647	22.940677349753933
11	33.936337850614564	23.518145894445926	24.48810880263764	18.057407452301874
12	22.963950641227665	25.62995466556765	30.63177289146404	20.774321801740648
13	27.91970714441562	22.210235399646052	26.194331983805668	23.675725472132658
14	22.085868748333294	21.144997454483743	27.51824286649373	29.250890930689227
15	23.99258163834275	28.84942665276734	24.243496812043926	22.914494896845987
16	22.858008678998278	28.53547964799147	25.335644500472736	23.270867172537514
17	22.276903682513517	27.538849426652767	26.52233993551359	23.661906955320127
18	22.890009454774663	27.164294891997383	27.882130475890328	22.063565177337633
19	24.38725787291813	26.728647967223445	27.083080801958836	21.801013357899585
20	24.23476932774128	26.3693665300977	27.242114960362674	22.153749181798347
21	23.24807874130282	27.17593153773425	27.319207738369418	22.25678198259352
22	22.837159688719726	26.958229290407036	27.07386845741715	23.13074256345608
23	23.068195592620427	26.852529758297173	27.604305558922643	22.47496909015976
24	23.24953332201993	27.347572062353027	26.107784431137727	23.29511018448932
25	23.42190113699726	26.98926034570535	27.497636306334698	22.09120221096269
26	23.277412785764504	27.084777812795462	27.106838953671602	22.530970447768432
27	23.123954520109578	27.1376275788504	26.79798298140561	22.940434919634413
28	22.71230817716793	26.931319547140536	27.267327692792552	23.08904458289898
29	23.68518024679386	26.9630778927974	26.837014230648016	22.51472762976072
30	23.489539140342796	26.633857790491888	27.47242357390482	22.40417949526049
31	23.600329704962544	26.715799170888992	26.937622730248005	22.74624839390046
32	22.483454144342893	26.9604111614827	27.25253945550195	23.303595238672454
33	22.515454920119275	26.98683604451017	27.810371160512982	22.687337874857572
34	23.132924434531745	26.419791994957453	27.358723847850857	23.088559722659944
35	22.836189968241655	27.214720356857136	27.208417173749666	22.740672501151543
36	23.828941307668064	26.837014230648016	26.802589153676454	22.531455308007466
37	23.06019539867633	26.58197774491503	27.116536158452327	23.241290697956313
38	23.121045358675364	26.82537758491115	26.94974423622391	23.10383282018958
39	22.79182525636985	26.693010739654294	27.55606196513855	22.959102038837305
40	22.738248199956363	27.279449198768457	27.400906688646998	22.581395912628185
41	22.991587674852724	26.100269097432665	27.40575529103736	23.502387936677252
42	22.525152124899996	27.149991514945814	27.317995587771826	23.00686077238236
43	23.067225872142355	26.02754006157725	27.639942786491794	23.2652912797886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	14.0
2	21.0
3	81.0
4	141.0
5	141.0
6	168.5
7	196.0
8	189.5
9	183.0
10	262.5
11	342.0
12	342.0
13	510.5
14	679.0
15	1199.0
16	1719.0
17	2042.0
18	2365.0
19	2365.0
20	2340.0
21	2315.0
22	2783.0
23	3251.0
24	4538.5
25	5826.0
26	5826.0
27	7105.0
28	8384.0
29	10244.0
30	12104.0
31	14287.5
32	16471.0
33	16471.0
34	18581.0
35	20691.0
36	22684.5
37	24678.0
38	27043.0
39	29408.0
40	29408.0
41	31355.0
42	33302.0
43	34023.0
44	34744.0
45	34125.0
46	33506.0
47	33506.0
48	32578.0
49	31650.0
50	31075.0
51	30500.0
52	29418.0
53	28336.0
54	28336.0
55	26346.5
56	24357.0
57	21516.5
58	18676.0
59	17039.5
60	15403.0
61	15403.0
62	13566.0
63	11729.0
64	9803.0
65	7877.0
66	6512.0
67	5147.0
68	5147.0
69	4269.0
70	3391.0
71	2753.5
72	2116.0
73	1712.0
74	1308.0
75	1308.0
76	996.0
77	684.0
78	541.5
79	399.0
80	328.5
81	258.0
82	258.0
83	203.5
84	149.0
85	122.5
86	96.0
87	73.5
88	51.0
89	51.0
90	36.5
91	22.0
92	14.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	412490.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.210207649506856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.10222245081867	55.68959775499294
2	6.56554220941748	8.300185727777619
3	1.8551936516744205	3.5180152785716103
4	0.8453221379491683	2.1373195148196804
5	0.4860393163601843	1.5361323056475802
6	0.341444633940309	1.2949671727306074
7	0.23740233831022495	1.0504375770747456
8	0.19217668459599316	0.9718022510965211
9	0.14280374236063778	0.8123988786968332
>10	1.0643729265933226	13.52130530826964
>50	0.11526330849563607	5.053130184085668
>100	0.05144302023229935	5.5804962477148505
>500	3.8678962580676206E-4	0.22004147307528435
>1k	3.8678962580676206E-4	0.31417032544637824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1285	0.31152270358069284	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	900	0.21818710756624404	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.11757860796625373	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	427	0.10351766103420688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.4243011951804894E-4	0.0	0.0
2	0.0	0.0	4.8486023903609787E-4	0.0	0.0
3	0.0	0.0	7.272903585541468E-4	0.0	0.0
4	0.0	0.0	9.697204780721957E-4	0.0	0.0
5	0.0	0.0	0.0012121505975902446	0.0	0.0
6	0.0	0.0	0.0019394409561443915	0.0	0.0
7	0.0	0.0	0.004121312031806832	0.0	0.0
8	0.0	0.0	0.004848602390360978	0.0	0.0
9	0.0	0.0	0.005575892748915125	4.8486023903609787E-4	0.0
10	0.0	0.0	0.006303183107469272	4.8486023903609787E-4	0.0
11	0.0	0.0	0.009939634900240005	4.8486023903609787E-4	0.0
12	0.0	0.0	0.010182065019758055	4.8486023903609787E-4	0.0
13	0.0	0.0	0.010909355378312202	7.272903585541468E-4	0.0
14	0.0	0.0	0.0113942156173483	7.272903585541468E-4	0.0
15	0.0	0.0	0.012121505975902447	7.272903585541468E-4	0.0
16	0.0	0.0	0.012848796334456593	7.272903585541468E-4	0.0
17	0.0	0.0	0.014060946932046837	7.272903585541468E-4	0.0
18	0.0	0.0	0.014303377051564887	7.272903585541468E-4	0.0
19	0.0	0.0	0.014545807171082935	7.272903585541468E-4	0.0
20	0.0	0.0	0.015273097529637082	7.272903585541468E-4	0.0
21	0.0	0.0	0.01600038788819123	9.697204780721957E-4	0.0
22	0.0	0.0	0.016242818007709277	9.697204780721957E-4	0.0
23	0.0	0.0	0.016242818007709277	9.697204780721957E-4	0.0
24	0.0	0.0	0.016242818007709277	0.0012121505975902446	0.0
25	0.0	0.0	0.016242818007709277	0.0014545807171082936	0.0
26	0.0	0.0	0.016242818007709277	0.0014545807171082936	0.0
27	0.0	0.0	0.016242818007709277	0.002424301195180489	0.0
28	0.0	0.0	0.016242818007709277	0.0113942156173483	0.0
29	0.0	0.0	0.016242818007709277	0.02884918422264782	0.0
30	0.0	0.0	0.016242818007709277	0.05381948653300686	0.0
31	0.0	0.0	0.016242818007709277	0.1229120705956508	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGATT	45	1.3212037E-4	24.666666	22
ACGTAGA	45	1.3212037E-4	24.666666	24
GTATCAA	680	0.0	24.213234	1
GTCCTAG	50	2.698034E-4	22.199999	1
CTGCTCG	145	0.0	21.689655	9
CGCTCTC	60	3.7188343E-5	21.583334	29
TGCTCGG	95	7.1122486E-9	21.421053	10
GCTCGGA	95	7.1122486E-9	21.421053	11
GGTATCA	280	0.0	20.482143	1
ACCGCAA	100	1.2838427E-8	20.349998	8
TTAACGG	100	1.2838427E-8	20.349998	35
CCTACGT	55	5.1354134E-4	20.181818	34
CTACGTG	55	5.1354134E-4	20.181818	35
TACGTGA	55	5.1354134E-4	20.181818	36
CTCGGAG	120	2.3646862E-10	20.041668	12
TTCGCCG	65	6.888425E-5	19.923077	24
ACAATGC	75	9.242169E-6	19.733334	8
TAACGGC	105	2.2489985E-8	19.38095	36
AACGGCC	105	2.2489985E-8	19.38095	37
GGTCGCC	135	5.4569682E-11	19.185186	36
>>END_MODULE