FastQCFastQC Report
Fri 10 Feb 2017
ERR1632816.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632816.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220438
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85383.8731979059871713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72373.2830092815213345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36691.6644135766065742No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.8709932044384363No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13610.6174071621045374No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.4527350093903955No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG9260.4200727642239542No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8190.37153303876827043No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7880.3574701276549415No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7560.3429535742476343No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7470.3388707936018291No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.2998575563196908No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5820.26401981509540096No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.2622052459194876No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5710.25902974986163907No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5340.24224498498444008No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.22863571616508951No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4930.22364565093132763No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4530.20549995917219355No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4490.20368538999628014No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4300.19506618641069146No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4140.1878079097070378No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4060.184178771355211No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4040.18327148676725427No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4010.1819105598853192No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4000.18145691759134086No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3990.18100327529736254No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3840.17419864088768722No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3740.1696622179479037No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.16739400647801195No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3610.16376486812618515No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3500.15877480289242327No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3480.15786751830446658No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.15514566454059645No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3380.15333109536468303No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3300.1497019570128562No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG3300.1497019570128562No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3300.1497019570128562No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3250.14743374554296446No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3240.1469801032489861No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3110.14108275342726753No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3080.13972182654533247No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3070.13926818425135412No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3070.13926818425135412No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG3040.13790725736941908No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3010.13654633048748402No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG3010.13654633048748402No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3000.13609268819350567No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2990.1356390458995273No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2930.1329171921356572No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2930.1329171921356572No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2900.13155626525372213No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA2890.1311026229597438No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.12928805378383038No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2770.12565891543200355No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2720.1233907039621118No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2710.12293706166813345No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.12248341937415509No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2690.12202977708017675No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2690.12202977708017675No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2670.12112249249222004No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2670.12112249249222004No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2600.11794699643437156No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG2550.11567878496447981No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2490.11295693120060969No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2400.10887415055480454No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2390.10842050826082618No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2380.10796686596684782No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2380.10796686596684782No Hit
TATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATGA2360.10705958137889111No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2300.104337727615021No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC2270.10297680073308596No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2230.10116223155717255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCAA451.0732401E-732.888891
TCGCTTC352.3796221E-531.71428730
TTAGATC250.0054868329.5999983
TTATGAC250.0054868329.5999983
GTATAAG250.0054868329.5999981
TGCGTCT250.0054868329.59999810
AAAGACT250.0054868329.59999836
CTAGTGC405.9187005E-527.7500029
CTTTATG405.9187005E-527.7500021
TAGTGCA405.9187005E-527.75000210
GTACGGA405.9187005E-527.7500021
TTTAGAT358.846572E-426.4285722
TTTATGA358.846572E-426.4285722
GTATTAT1000.025.8999981
GGTATCA12150.025.5802461
TATTATA1002.0008883E-1124.052
GCCGGCA3600.023.6388915
GCTCTCT3650.023.3150730
CGCTCTC3650.023.3150729
GCCGCTC3750.023.18666527
Produced by FastQC (version 0.11.5)