FastQCFastQC Report
Fri 10 Feb 2017
ERR1632815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328000
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9650.2942073170731707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.22743902439024388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3760.1146341463414634No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3730.11371951219512194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA905.456968E-1226.72222129
GGTATCA2000.025.9000021
GCGAACT1054.0017767E-1122.9047628
ACACTCC652.6731504E-622.769238
AGCGAAC1107.2759576E-1121.86363627
GTATCAA5350.021.7850461
ATGGGCG450.003820069420.55555511
CACGACA450.003820069420.55555519
CGACACG450.003820069420.55555521
TCTACAC450.003820069420.5555553
CTACACG450.003820069420.55555516
ACGTACT450.003820069420.55555525
TACACGA450.003820069420.55555517
ACGACAC450.003820069420.55555520
GAACTAC1202.3646862E-1020.04166630
CAAAGCG1254.0927262E-1019.2424
ACACGTA500.00702442918.523
GTATTAG1306.89397E-1018.51
TAGACAG1002.8593968E-718.55
CGTTGTA609.217646E-418.533
Produced by FastQC (version 0.11.5)