FastQCFastQC Report
Fri 10 Feb 2017
ERR1632814.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632814.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337122
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11450.3396396556736137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.2616263548507662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4630.1373390048706403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAT358.8563433E-426.4285722
TTCGGTC358.8563433E-426.4285728
GGTATCA2750.026.2363641
GTATCAA7150.023.8041951
ATAGGGG400.001928372823.1253
CCATCGG400.001928372823.12519
TTTTCGG400.001928372823.12526
ACGGGTC502.6966163E-422.216
TCGAGGT502.6966163E-422.215
AGGTCGT502.6966163E-422.218
TCTATAC705.0842937E-621.1428573
ACGGGCT450.003820285220.55555516
CGGTCAC450.003820285220.55555530
GGTCGTA555.1327364E-420.18181819
TTTTTCG555.1327364E-420.18181825
AACGGCC656.883653E-519.92307737
CGGGTCA500.007024823718.517
TACCAAC609.21837E-418.57
ACCATCG500.007024823718.518
AACATCG609.21837E-418.511
Produced by FastQC (version 0.11.5)