##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632814.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	337122
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7259211798696	33.0	33.0	33.0	27.0	33.0
2	31.223405770017976	33.0	33.0	33.0	27.0	33.0
3	31.68274393246362	33.0	33.0	33.0	27.0	33.0
4	31.81384187326843	33.0	33.0	33.0	27.0	33.0
5	31.92439235647629	33.0	33.0	33.0	33.0	33.0
6	34.832538368899094	37.0	37.0	37.0	27.0	37.0
7	34.99767739868653	37.0	37.0	37.0	33.0	37.0
8	35.17169452008472	37.0	37.0	37.0	33.0	37.0
9	35.26077206471248	37.0	37.0	37.0	33.0	37.0
10	35.29085612923512	37.0	37.0	37.0	33.0	37.0
11	35.30998570250532	37.0	37.0	37.0	33.0	37.0
12	35.28967258143936	37.0	37.0	37.0	33.0	37.0
13	35.32958394883751	37.0	37.0	37.0	33.0	37.0
14	35.270611232728804	37.0	37.0	37.0	33.0	37.0
15	35.29371859445542	37.0	37.0	37.0	33.0	37.0
16	35.29869008845463	37.0	37.0	37.0	33.0	37.0
17	35.250591773897874	37.0	37.0	37.0	33.0	37.0
18	35.2785519782156	37.0	37.0	37.0	33.0	37.0
19	35.28142334229152	37.0	37.0	37.0	33.0	37.0
20	35.29217019357977	37.0	37.0	37.0	33.0	37.0
21	35.28048006359715	37.0	37.0	37.0	33.0	37.0
22	35.284377762353095	37.0	37.0	37.0	33.0	37.0
23	35.27167316283126	37.0	37.0	37.0	33.0	37.0
24	35.26975694259052	37.0	37.0	37.0	33.0	37.0
25	35.25874015934884	37.0	37.0	37.0	33.0	37.0
26	35.2100278237552	37.0	37.0	37.0	33.0	37.0
27	35.1570321723293	37.0	37.0	37.0	33.0	37.0
28	35.15135173616673	37.0	37.0	37.0	33.0	37.0
29	35.172272945699184	37.0	37.0	37.0	33.0	37.0
30	35.13761190310926	37.0	37.0	37.0	33.0	37.0
31	35.14044767176274	37.0	37.0	37.0	33.0	37.0
32	35.15854794406773	37.0	37.0	37.0	33.0	37.0
33	35.12661885014921	37.0	37.0	37.0	33.0	37.0
34	35.06794276256073	37.0	37.0	37.0	33.0	37.0
35	35.06177882191017	37.0	37.0	37.0	27.0	37.0
36	35.04922550293366	37.0	37.0	37.0	27.0	37.0
37	35.07378931069464	37.0	37.0	37.0	33.0	37.0
38	34.91560918599202	37.0	37.0	37.0	27.0	37.0
39	34.98265909670683	37.0	37.0	37.0	27.0	37.0
40	35.02200983620173	37.0	37.0	37.0	27.0	37.0
41	35.028645416199474	37.0	37.0	37.0	27.0	37.0
42	34.93935429903714	37.0	37.0	37.0	27.0	37.0
43	34.72102087671526	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	19.0
17	14.0
18	9.0
19	10.0
20	16.0
21	60.0
22	170.0
23	415.0
24	874.0
25	1662.0
26	2711.0
27	3980.0
28	5732.0
29	7853.0
30	10153.0
31	13200.0
32	16639.0
33	21981.0
34	31149.0
35	57800.0
36	162667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.2714744217227	21.222584109016914	13.907428171403824	24.598513297856563
2	15.357645006852119	23.844483599409116	38.63141533332147	22.166456060417296
3	17.492183838491705	27.385041616981386	31.09883069037322	24.02394385415369
4	12.37415535028862	18.28002918824639	40.09379393809956	29.25202152336543
5	13.346800268152181	37.561772889339764	35.98252264758752	13.108904194920534
6	28.500661481600133	40.61259721999751	17.967975984955	12.91876531344736
7	25.43797201013283	32.78071440012814	24.381974478082117	17.399339111656907
8	23.736214189521895	36.466917021137746	21.54412942495595	18.252739364384407
9	25.53793582145336	15.674444266467333	20.858027657643227	37.92959225443608
10	14.133755732346154	28.897550441679865	34.90309146243793	22.065602363536048
11	32.7697391448793	24.217345649349493	24.936966439449222	18.075948766321982
12	22.086366359952777	26.048730133304858	31.460124228024277	20.40477927871809
13	27.40283932819573	22.644917863562746	27.215963360445176	22.736279447796345
14	21.525738456701134	21.71380093853264	28.46091325988811	28.299547344878118
15	23.001761973410222	29.613315061016486	25.072229044678192	22.312693920895107
16	22.26345358653544	29.458474973451747	25.716209562116976	22.561861877895836
17	21.61621015537402	28.383196587585502	27.20409821963562	22.796495037404856
18	22.3370174595547	27.89464941475193	28.40099429879984	21.36733882689353
19	23.84685662757103	26.97599088757186	28.313785513849588	20.86336697100753
20	23.767360184146984	26.981626829456403	28.079448982860804	21.17156400353581
21	22.291929924478378	27.803584459038568	27.82494171249577	22.079543903987283
22	21.797153552719788	27.699171219914454	28.086864695866776	22.41681053149898
23	21.94457792727855	27.68552630798346	28.31823494165317	22.051660823084816
24	22.479399149269405	27.5998006656344	27.57607038401528	22.344729801080916
25	22.4352014997538	27.583486097021254	28.16072519740628	21.820587205818665
26	22.384181394272694	27.68849259318585	27.894352786231693	22.032973226309764
27	22.426302644146627	27.715782417047834	27.509032338441276	22.34888260036426
28	21.954366668446436	27.44466394954942	28.391205557631956	22.209763824372185
29	22.792935495161988	27.528906449297285	27.73921607014671	21.93894198539401
30	22.329898375068964	27.772141835893237	27.993426711991503	21.904533077046292
31	22.58944833027806	27.47580994417451	27.96673014517	21.968011580377432
32	21.618879812056168	27.495090797990045	28.070253498733393	22.815775891220387
33	21.724182936741002	27.402542699675486	28.686647563789965	22.186626799793547
34	22.126114581664797	27.228718386815455	28.233102556344587	22.412064475175157
35	22.17624480158518	27.89998872811623	27.98541774194505	21.938348728353535
36	22.404055505128706	27.67692408089653	27.848671994114888	22.070348419859872
37	22.639281921678204	27.28656094826205	27.696204934712064	22.377952195347678
38	22.56927759090181	27.45712234739946	27.912447125966267	22.061152935732466
39	22.188999827955456	27.35033608011343	28.135808401706207	22.324855690224904
40	22.010132830251365	27.65349042779765	28.32594728317938	22.0104294587716
41	22.046618138240756	26.92853032433362	28.30992934308648	22.71492219433914
42	22.075687733224175	27.494200912429328	28.115637662329956	22.31447369201654
43	22.293413067079573	26.88907873114184	28.169030795972972	22.648477405805615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	15.5
2	20.0
3	81.0
4	142.0
5	142.0
6	183.0
7	224.0
8	210.0
9	196.0
10	268.0
11	340.0
12	340.0
13	546.5
14	753.0
15	1301.5
16	1850.0
17	2188.5
18	2527.0
19	2527.0
20	2617.5
21	2708.0
22	3346.0
23	3984.0
24	5236.0
25	6488.0
26	6488.0
27	7997.0
28	9506.0
29	11425.5
30	13345.0
31	14996.0
32	16647.0
33	16647.0
34	18113.0
35	19579.0
36	21153.0
37	22727.0
38	23924.5
39	25122.0
40	25122.0
41	25643.5
42	26165.0
43	25704.0
44	25243.0
45	24819.5
46	24396.0
47	24396.0
48	23620.0
49	22844.0
50	22757.0
51	22670.0
52	22353.5
53	22037.0
54	22037.0
55	20209.0
56	18381.0
57	16336.5
58	14292.0
59	12764.0
60	11236.0
61	11236.0
62	9713.5
63	8191.0
64	6758.0
65	5325.0
66	4507.5
67	3690.0
68	3690.0
69	3044.0
70	2398.0
71	2071.5
72	1745.0
73	1388.5
74	1032.0
75	1032.0
76	792.5
77	553.0
78	420.0
79	287.0
80	227.5
81	168.0
82	168.0
83	140.5
84	113.0
85	104.0
86	95.0
87	73.0
88	51.0
89	51.0
90	44.0
91	37.0
92	20.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	337122.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.90228214012635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.18916662569201	65.56709186843501
2	4.915237733725458	7.068336206322462
3	1.316429016745611	2.8396275173847614
4	0.6095826173523125	1.753215253623306
5	0.36944132410448177	1.3281837159991157
6	0.24591496179840497	1.0609108179424385
7	0.1946112527337903	0.9795095241195898
8	0.14583526990276596	0.8388710978024124
9	0.10302836103775388	0.6667176855394242
>10	0.8177570340827427	11.421673493299666
>50	0.06767105294839174	3.2636032177565117
>100	0.024494430208252597	2.6071810576996155
>500	4.151598340381796E-4	0.263285286568704
>1k	4.151598340381796E-4	0.34179325750699097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1145	0.3396396556736137	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	882	0.2616263548507662	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	463	0.1373390048706403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.9662852023896395E-4	0.0	0.0
2	2.9662852023896395E-4	0.0	2.9662852023896395E-4	0.0	0.0
3	2.9662852023896395E-4	0.0	2.9662852023896395E-4	0.0	0.0
4	2.9662852023896395E-4	0.0	5.932570404779279E-4	0.0	0.0
5	2.9662852023896395E-4	0.0	8.898855607168918E-4	0.0	0.0
6	2.9662852023896395E-4	0.0	8.898855607168918E-4	0.0	0.0
7	2.9662852023896395E-4	0.0	0.0023730281619117116	0.0	0.0
8	2.9662852023896395E-4	0.0	0.0029662852023896393	0.0	0.0
9	2.9662852023896395E-4	0.0	0.004746056323823423	0.0011865140809558558	0.0
10	2.9662852023896395E-4	0.0	0.00533931336430135	0.0011865140809558558	0.0
11	2.9662852023896395E-4	0.0	0.008602227086929953	0.0011865140809558558	0.0
12	2.9662852023896395E-4	0.0	0.008602227086929953	0.0011865140809558558	0.0
13	2.9662852023896395E-4	0.0	0.008898855607168918	0.0011865140809558558	0.0
14	2.9662852023896395E-4	0.0	0.009195484127407881	0.0011865140809558558	0.0
15	2.9662852023896395E-4	0.0	0.010381998208363738	0.0011865140809558558	0.0
16	2.9662852023896395E-4	0.0	0.011271883769080629	0.0011865140809558558	0.0
17	2.9662852023896395E-4	0.0	0.011865140809558557	0.0011865140809558558	0.0
18	2.9662852023896395E-4	0.0	0.012161769329797522	0.0011865140809558558	0.0
19	5.932570404779279E-4	0.0	0.01275502637027545	0.0014831426011948196	0.0
20	5.932570404779279E-4	0.0	0.013051654890514413	0.0014831426011948196	0.0
21	5.932570404779279E-4	0.0	0.013051654890514413	0.0017797711214337837	0.0
22	5.932570404779279E-4	0.0	0.013051654890514413	0.0017797711214337837	0.0
23	5.932570404779279E-4	0.0	0.013051654890514413	0.0020763996416727475	0.0
24	5.932570404779279E-4	0.0	0.013051654890514413	0.0020763996416727475	0.0
25	5.932570404779279E-4	0.0	0.013051654890514413	0.0020763996416727475	0.0
26	5.932570404779279E-4	0.0	0.013051654890514413	0.0020763996416727475	0.0
27	5.932570404779279E-4	0.0	0.013051654890514413	0.0035595422428675674	0.0
28	5.932570404779279E-4	0.0	0.013051654890514413	0.013348283410753376	0.0
29	5.932570404779279E-4	0.0	0.013051654890514413	0.031145994625091215	0.0
30	5.932570404779279E-4	0.0	0.013051654890514413	0.058435818487075894	0.0
31	5.932570404779279E-4	0.0	0.013051654890514413	0.11538849437295697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGAT	35	8.8563433E-4	26.42857	22
TTCGGTC	35	8.8563433E-4	26.42857	28
GGTATCA	275	0.0	26.236364	1
GTATCAA	715	0.0	23.804195	1
ATAGGGG	40	0.0019283728	23.125	3
CCATCGG	40	0.0019283728	23.125	19
TTTTCGG	40	0.0019283728	23.125	26
ACGGGTC	50	2.6966163E-4	22.2	16
TCGAGGT	50	2.6966163E-4	22.2	15
AGGTCGT	50	2.6966163E-4	22.2	18
TCTATAC	70	5.0842937E-6	21.142857	3
ACGGGCT	45	0.0038202852	20.555555	16
CGGTCAC	45	0.0038202852	20.555555	30
GGTCGTA	55	5.1327364E-4	20.181818	19
TTTTTCG	55	5.1327364E-4	20.181818	25
AACGGCC	65	6.883653E-5	19.923077	37
CGGGTCA	50	0.0070248237	18.5	17
TACCAAC	60	9.21837E-4	18.5	7
ACCATCG	50	0.0070248237	18.5	18
AACATCG	60	9.21837E-4	18.5	11
>>END_MODULE