FastQCFastQC Report
Fri 10 Feb 2017
ERR1632813.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632813.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283833
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5120.18038776322696798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.14163257972117407No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3260.11485627111717102No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3170.11168539246669697No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC2990.10534363516574888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG250.00548945729.61
GTATCAA3150.022.317461
CTAGACT603.713572E-521.5833344
AATTAGT450.00381881920.5555552
GGTATCA1202.3646862E-1020.0416681
TAGACTG759.225512E-619.7333345
TAGGACC851.238559E-619.5882364
TAGAGGG951.6650301E-719.4736845
GTCAACC951.6650301E-719.47368415
ATACTGT701.2150963E-418.56
CATTAGT609.213469E-418.51
CTTATAG500.007022152718.53
TACTAAG500.007022152718.57
TATACTG500.007022152718.55
AGAGGGT500.007022152718.56
TCAAGAC801.6096908E-518.53
TCAACCC1002.8560135E-718.516
ATCTATC650.001575974317.07692136
TGTCAAC1107.756771E-716.81818214
AGTCCTG1005.8464775E-616.6500025
Produced by FastQC (version 0.11.5)