FastQCFastQC Report
Fri 10 Feb 2017
ERR1632812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260286
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6110.2347417840375587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.1744235187447654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA250.0054886329.62
TGCCGAG250.0054886329.611
GTCTAGA601.3307763E-624.6666661
GGTATCA1350.023.2962971
ACTGATC400.001927197623.1258
GTATCAA3200.023.1251
TCTAGAC1059.731593E-1021.1428573
TATGAGC450.003817978320.5555576
CGGAGTC450.003817978320.55555727
TTCCGTT555.128414E-420.18181836
CTCTTAC555.128414E-420.18181831
CGCTGTT555.128414E-420.18181812
GCAATTA555.128414E-420.18181817
GCGCTGT656.875943E-519.92307711
ACAACGG500.007020623418.523
GACGTAT609.210671E-418.526
ACGGGAC701.214605E-418.516
AACGGAG609.210671E-418.525
TGCGCTG609.210671E-418.510
ACATGTA902.139228E-618.58
Produced by FastQC (version 0.11.5)