##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632812.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260286
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72670062930776	33.0	33.0	33.0	27.0	33.0
2	31.242571632742443	33.0	33.0	33.0	27.0	33.0
3	31.67796577610782	33.0	33.0	33.0	27.0	33.0
4	31.81895683978393	33.0	33.0	33.0	27.0	33.0
5	31.930288221417978	33.0	33.0	33.0	33.0	33.0
6	34.84356438686675	37.0	37.0	37.0	27.0	37.0
7	35.010265630882955	37.0	37.0	37.0	33.0	37.0
8	35.180950953950656	37.0	37.0	37.0	33.0	37.0
9	35.26774010127321	37.0	37.0	37.0	33.0	37.0
10	35.29792228548597	37.0	37.0	37.0	33.0	37.0
11	35.33341401381557	37.0	37.0	37.0	33.0	37.0
12	35.298955764044166	37.0	37.0	37.0	33.0	37.0
13	35.32474662486649	37.0	37.0	37.0	33.0	37.0
14	35.295920641140896	37.0	37.0	37.0	33.0	37.0
15	35.30893709227542	37.0	37.0	37.0	33.0	37.0
16	35.31003972553268	37.0	37.0	37.0	33.0	37.0
17	35.26804745549127	37.0	37.0	37.0	33.0	37.0
18	35.278731856496314	37.0	37.0	37.0	33.0	37.0
19	35.27427137840683	37.0	37.0	37.0	33.0	37.0
20	35.27677631528395	37.0	37.0	37.0	33.0	37.0
21	35.293488700890556	37.0	37.0	37.0	33.0	37.0
22	35.28670385652705	37.0	37.0	37.0	33.0	37.0
23	35.271966221771436	37.0	37.0	37.0	33.0	37.0
24	35.286965107612396	37.0	37.0	37.0	33.0	37.0
25	35.29003864979292	37.0	37.0	37.0	33.0	37.0
26	35.25617974074672	37.0	37.0	37.0	33.0	37.0
27	35.189622184827456	37.0	37.0	37.0	33.0	37.0
28	35.16279784544693	37.0	37.0	37.0	33.0	37.0
29	35.20092513619634	37.0	37.0	37.0	33.0	37.0
30	35.1736051881392	37.0	37.0	37.0	33.0	37.0
31	35.16271716496469	37.0	37.0	37.0	33.0	37.0
32	35.18528080649747	37.0	37.0	37.0	33.0	37.0
33	35.159593677723734	37.0	37.0	37.0	33.0	37.0
34	35.10907617005909	37.0	37.0	37.0	33.0	37.0
35	35.08517169575006	37.0	37.0	37.0	33.0	37.0
36	35.07197467401243	37.0	37.0	37.0	33.0	37.0
37	35.105107458718486	37.0	37.0	37.0	33.0	37.0
38	34.970155905427106	37.0	37.0	37.0	27.0	37.0
39	35.03846538038926	37.0	37.0	37.0	27.0	37.0
40	35.074810016673965	37.0	37.0	37.0	33.0	37.0
41	35.09734676471266	37.0	37.0	37.0	33.0	37.0
42	35.00547090508133	37.0	37.0	37.0	27.0	37.0
43	34.827263087526795	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	14.0
17	10.0
18	9.0
19	10.0
20	15.0
21	49.0
22	149.0
23	335.0
24	698.0
25	1182.0
26	1984.0
27	3074.0
28	4386.0
29	5952.0
30	7705.0
31	9878.0
32	12792.0
33	16545.0
34	24244.0
35	44387.0
36	126865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30648594238645	20.13054870411778	13.023750797200004	25.539214556295768
2	16.439992930852984	22.83603420852447	36.91093643146385	23.813036429158696
3	18.234941564279293	25.137733108964756	30.35353418931483	26.273791137441123
4	13.359535280422305	17.16342792159394	38.158794556756796	31.318242241226958
5	14.403387043482937	36.561705201201754	35.07603174969072	13.958876005624582
6	31.03509216784614	38.730473402334354	16.98593086066865	13.248503569150857
7	27.735644637053085	31.602544892925472	22.795693967405086	17.866116502616354
8	25.07242033762861	35.54551531776584	20.49245829587454	18.88960604873101
9	25.960289835027623	15.047678323075386	20.01260152294015	38.979430318956844
10	14.506350706530508	28.322691193533267	34.07213603497691	23.098822064959315
11	34.413683409787694	22.92247758235172	24.38740462414421	18.27643438371637
12	23.43768009036214	25.26451672391139	30.154906525898433	21.142896659828036
13	28.320001844125308	21.498659167223746	26.341024872640094	23.84031411601085
14	22.457220134774825	20.275005186602428	26.950738802701647	30.3170358759211
15	23.698546982934158	29.05112069031757	23.66204866954043	23.58828365720784
16	23.701236332342116	28.241626518521933	24.47500057628916	23.582136572846792
17	22.998163558547134	27.55353726285701	25.97719431702051	23.471104861575345
18	23.698931175706722	26.910014368809694	27.003757405315692	22.38729705016789
19	24.62560414313486	26.401727330705455	26.558862174684773	22.413806351474914
20	24.01358505643792	26.835480970931975	26.591134367580278	22.55979960504983
21	23.315890981458857	26.786688488816147	27.295359719692954	22.602060810032043
22	23.279008475292564	26.41709504160808	26.988005501640504	23.315890981458857
23	23.25595690893863	26.337567137687007	26.86621639273722	23.540259560637146
24	22.866001244784584	26.808971669624952	26.344482607593182	23.980544477997277
25	23.701236332342116	26.71714959698178	26.712155090938428	22.869458979737672
26	23.852992477505513	26.346403571456012	26.574229885587393	23.22637406545108
27	23.40118177696841	26.653373596735896	26.346403571456012	23.599041054839674
28	22.96320201624367	26.173132631028945	27.258093020754092	23.605572331973292
29	23.626318741691833	26.30606333033663	26.38328607762231	23.68433185034923
30	23.176044812244992	26.77593109118431	26.92422950139462	23.123794595176076
31	23.53910698231945	26.43323113805583	26.799751043083376	23.227910836541344
32	23.165287414613157	26.550794126460893	26.903098898903515	23.380819560022438
33	23.14415681212205	26.56155152409273	27.25002497253022	23.044266691255004
34	23.821872862927705	25.789708244008512	26.75864241641886	23.62977647664492
35	23.056176667204536	26.88888376631859	27.014130610174963	23.040808956301913
36	24.134221587023504	25.875383232290634	27.067149212789012	22.92324596789685
37	23.747339465049983	26.08207894393091	26.681419669133184	23.489161921885927
38	23.85683440523117	26.250355378314623	26.793988151494897	23.098822064959315
39	23.391961150426837	26.38251769207718	27.041792489799683	23.183728667696304
40	23.26095141498198	26.957654272607822	27.044097646435077	22.73729666597512
41	23.768470067541088	25.84311103939513	26.930376585755667	23.458042307308116
42	23.209853776230762	26.515064198612297	26.953428152109606	23.32165387304734
43	23.559469199265422	25.73092674980598	27.06791759833414	23.641686452594453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	11.0
2	15.0
3	40.0
4	65.0
5	65.0
6	83.0
7	101.0
8	97.5
9	94.0
10	129.5
11	165.0
12	165.0
13	240.0
14	315.0
15	598.0
16	881.0
17	1010.5
18	1140.0
19	1140.0
20	1268.5
21	1397.0
22	1635.0
23	1873.0
24	2416.0
25	2959.0
26	2959.0
27	3817.0
28	4675.0
29	5568.0
30	6461.0
31	7946.0
32	9431.0
33	9431.0
34	11204.5
35	12978.0
36	14253.5
37	15529.0
38	16686.0
39	17843.0
40	17843.0
41	18397.0
42	18951.0
43	19881.0
44	20811.0
45	21056.5
46	21302.0
47	21302.0
48	20410.0
49	19518.0
50	19745.5
51	19973.0
52	19184.0
53	18395.0
54	18395.0
55	17389.5
56	16384.0
57	14471.0
58	12558.0
59	11926.5
60	11295.0
61	11295.0
62	10453.0
63	9611.0
64	7857.0
65	6103.0
66	5014.5
67	3926.0
68	3926.0
69	3114.5
70	2303.0
71	1854.0
72	1405.0
73	1119.5
74	834.0
75	834.0
76	612.5
77	391.0
78	304.5
79	218.0
80	177.0
81	136.0
82	136.0
83	117.0
84	98.0
85	92.0
86	86.0
87	63.5
88	41.0
89	41.0
90	28.5
91	16.0
92	8.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	260286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.45580292571952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6055142958474	54.09016933142029
2	6.339780562389896	7.919121707938611
3	2.2295860045820377	4.1775175232435435
4	1.1737002338778482	2.9321756200378317
5	0.7248152835551522	2.2634460253635047
6	0.49843828126425893	1.8678217839164946
7	0.36816506838091856	1.6095831468452888
8	0.2773790993586343	1.385914749220515
9	0.2238388504413521	1.2582031617256224
>10	1.4643888212878222	17.079532492428253
>50	0.06987570706282177	2.9243588906348856
>100	0.023904847153070608	2.258252794325853
>500	6.129447987966823E-4	0.23390277289932127
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	611	0.2347417840375587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	454	0.1744235187447654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	3.841927725655625E-4	0.0	0.0
9	0.0	0.0	0.00153677109026225	3.841927725655625E-4	0.0
10	0.0	0.0	0.0019209638628278124	3.841927725655625E-4	0.0
11	0.0	0.0	0.0026893494079589376	3.841927725655625E-4	0.0
12	0.0	0.0	0.0026893494079589376	3.841927725655625E-4	0.0
13	0.0	0.0	0.0030735421805245	3.841927725655625E-4	0.0
14	0.0	0.0	0.0030735421805245	3.841927725655625E-4	0.0
15	0.0	0.0	0.0038419277256556248	3.841927725655625E-4	0.0
16	0.0	0.0	0.004226120498221187	3.841927725655625E-4	0.0
17	0.0	0.0	0.004226120498221187	3.841927725655625E-4	0.0
18	0.0	0.0	0.004226120498221187	7.68385545131125E-4	0.0
19	0.0	0.0	0.004226120498221187	7.68385545131125E-4	0.0
20	0.0	0.0	0.004226120498221187	0.0011525783176966876	0.0
21	0.0	0.0	0.004226120498221187	0.0011525783176966876	3.841927725655625E-4
22	0.0	0.0	0.004226120498221187	0.0011525783176966876	3.841927725655625E-4
23	0.0	0.0	0.004226120498221187	0.00153677109026225	3.841927725655625E-4
24	0.0	0.0	0.004226120498221187	0.00153677109026225	3.841927725655625E-4
25	0.0	0.0	0.004226120498221187	0.0019209638628278124	3.841927725655625E-4
26	0.0	0.0	0.004226120498221187	0.002305156635393375	3.841927725655625E-4
27	0.0	0.0	0.004226120498221187	0.0034577349530900627	3.841927725655625E-4
28	0.0	0.0	0.004226120498221187	0.0092206265415735	3.841927725655625E-4
29	0.0	0.0	0.004226120498221187	0.01997802417340925	3.841927725655625E-4
30	0.0	0.0	0.004226120498221187	0.03726669893885956	3.841927725655625E-4
31	0.0	0.0	0.004226120498221187	0.07837532560337475	3.841927725655625E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAATA	25	0.00548863	29.6	2
TGCCGAG	25	0.00548863	29.6	11
GTCTAGA	60	1.3307763E-6	24.666666	1
GGTATCA	135	0.0	23.296297	1
ACTGATC	40	0.0019271976	23.125	8
GTATCAA	320	0.0	23.125	1
TCTAGAC	105	9.731593E-10	21.142857	3
TATGAGC	45	0.0038179783	20.555557	6
CGGAGTC	45	0.0038179783	20.555557	27
TTCCGTT	55	5.128414E-4	20.181818	36
CTCTTAC	55	5.128414E-4	20.181818	31
CGCTGTT	55	5.128414E-4	20.181818	12
GCAATTA	55	5.128414E-4	20.181818	17
GCGCTGT	65	6.875943E-5	19.923077	11
ACAACGG	50	0.0070206234	18.5	23
GACGTAT	60	9.210671E-4	18.5	26
ACGGGAC	70	1.214605E-4	18.5	16
AACGGAG	60	9.210671E-4	18.5	25
TGCGCTG	60	9.210671E-4	18.5	10
ACATGTA	90	2.139228E-6	18.5	8
>>END_MODULE