FastQCFastQC Report
Fri 10 Feb 2017
ERR1632811.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632811.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411436
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18400.4472141475223364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.34245909448857176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6780.1647886913152957No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6490.15774020746847625No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG5650.13732390942941308No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5510.13392119308956923No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG5500.13367814192243752No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5090.12371304407003762No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5000.12152558356585227No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4950.12031032773019376No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4940.12006727656306206No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4860.11812286722600843No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4670.11350489505050604No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT4580.11131743454632069No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4420.10742861587221342No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4250.10329674603097444No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC4210.10232454136244762No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4160.10110928552678909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTT250.005492293329.59999820
GTATCAA8750.025.161
TAATAGT400.001929091523.1254
GGTCACA400.001929091523.1251
TGCACCG400.001929091523.1255
GGTATCA2950.022.5762711
CGAGTCG502.698018E-422.19999921
GAGTCGC603.7188045E-521.58333422
AGTCGCC603.7188045E-521.58333423
TTTACCG450.003821695720.55555730
GACCGTT450.003821695720.5555577
ACGGCTA450.003821695720.55555737
CATAATA450.003821695720.5555572
TTACCGG450.003821695720.55555731
TGTCCGG909.4412826E-820.55555714
GTACTTA656.888372E-519.9230771
GTATAAA656.888372E-519.9230771
CTAATAC1500.019.7333343
GCCGCTC1052.2488166E-819.3809527
GTATTAG1355.4569682E-1119.1851861
Produced by FastQC (version 0.11.5)