FastQCFastQC Report
Fri 10 Feb 2017
ERR1632810.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632810.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397091
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37680.9489008816618861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29230.7361033113316595No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14100.35508233629067393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13250.33367666353556236No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12520.3152929681105842No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11900.2996794185715617No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9890.2490612982918273No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9080.2286629513134269No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8460.21304940177440435No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8180.20599812133742643No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8110.20423530122818195No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG8050.20272431256311524No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7830.19718402079120403No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7730.1946657063494262No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7700.19391021201689285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.19239922335182616No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7290.18358512280560374No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7280.18333329136142598No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7180.18081497691964815No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG7050.17754116814533696No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6820.17174904492924797No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6740.1697343933758257No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6720.16923073048747014No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6300.15865380983200325No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6260.1576464840552921No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5980.1505952036183142No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5900.14858055206489193No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5890.14832872062071414No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5850.14732139484400303No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5760.14505491184640296No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5740.14455124895804738No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5600.14102560873955844No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5560.14001828296284732No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5560.14001828296284732No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA5560.14001828296284732No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT5530.13926278863031397No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT5490.13825546285360282No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT5440.13699630563271392No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5410.13624081130018056No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG5360.13498165407929166No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA5320.13397432830258052No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG5310.13372249685840273No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC5290.13321883397004716No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5240.13195967674915826No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA5210.1312041824166249No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5160.12994502519573597No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5100.12843403653066932No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC5020.12641938497724703No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5010.12616755353306924No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4960.12490839631218033No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4890.12314557620293584No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4880.12289374475875807No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCG4860.1223900818704025No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4860.1223900818704025No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAA4840.12188641898204693No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT4790.12062726176115801No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG4760.11987176742862468No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4670.11760528443102462No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT4660.11735345298684685No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4600.11584246432178014No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4460.11231682410329119No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT4440.11181316121493562No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG4430.11156132977075783No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA4410.11105766688240228No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGC4310.10853935244062443No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGAT4270.10753202666391332No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4220.1062728694430244No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4200.10576920655466883No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.10350272355706877No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT4100.103250892112891No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4100.103250892112891No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTC4100.103250892112891No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA4100.103250892112891No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4070.10249539778035766No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATG4040.1017399034478243No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4020.10123624055946873No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC3980.10022891478275761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTC200.001840485437.024
AGGTGCT200.001840485437.010
GGTATCA5500.030.6090931
CTGTCGT250.00549206529.623
AGGAGCG250.00549206529.67
TATCTCG250.00549206529.65
CCGACAA250.00549206529.633
AGCCACG405.9304894E-527.7537
GCTCTCT950.027.26315930
CGCTCTC950.027.26315929
GCTGTCG358.859132E-426.4285722
CCGCTCT1000.025.90000228
CTCTCTT1100.025.22727231
CTGAGCG701.9163417E-723.78571334
TGAGCGT701.9163417E-723.78571335
GCCGCTC1103.6379788E-1223.54545427
GCCGGCA1200.023.12500215
CCCTCGG400.001928973523.12521
GGTAGAC400.001928973523.1253
AAAGACG400.001928973523.12514
Produced by FastQC (version 0.11.5)