FastQCFastQC Report
Fri 10 Feb 2017
ERR1632809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454217
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14980.32979831226043943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11330.24944024552141378No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7740.17040313330412554No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7650.16842170152151945No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7080.15587263356501407No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6910.1521299290867581No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6550.14420420195633366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6280.1382599066085153No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6060.13341640669547813No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5650.12438988413027253No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT5310.11690447517376057No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5310.11690447517376057No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5290.11646415699984808No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5210.11470288430419821No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG4920.10831827078246742No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATT4670.10281429360856154No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA4640.10215381634769284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC303.596082E-430.8333323
GTCGCAA250.00549288829.59999818
ATACCGC250.00549288829.5999986
AGTCGCC751.3691533E-824.66666623
CGAGTCG802.7166607E-823.12500221
GGTATCA3050.023.049181
AACGGCC1300.022.7692337
TTAACGG1300.022.7692335
TCGCCGT753.7304744E-722.225
TTGTACT753.7304744E-722.24
GTATCAA8750.022.21
TAACGGC1350.021.92592636
CTCTAAT1700.021.7647061
TACCGCA957.1158865E-921.4210537
ATGCTAG1650.021.3030315
TTTAACG1400.021.14285734
GAGTCGC806.9410635E-720.812522
GTCGCCG909.446558E-820.55555724
ATAACAC450.003822298720.5555573
TCGAGCC450.003822298720.55555718
Produced by FastQC (version 0.11.5)