FastQCFastQC Report
Fri 10 Feb 2017
ERR1632808.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632808.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364368
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7110.19513239362402846No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5620.1542396697843938No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5590.15341632635138103No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5360.14710402669828304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.13804724893514247No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4780.131186053660036No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC4560.12514820181794228No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3830.10511351161463137No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3790.10401572037061432No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC3730.10236903350458876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1600.026.5937521
GTATCAA4550.023.1758231
CCGTACA603.7173006E-521.58333417
GCTCGGA806.9332964E-720.812511
AACCGGG450.003820870320.55555533
CGAACCG450.003820870320.55555531
ACGTTGC450.003820870320.55555525
ATTATAC450.003820870320.5555553
CGCAATA450.003820870320.55555534
ATACTAC555.133834E-420.1818183
GCCCCGT656.885605E-519.92307714
CCCCTAT1103.830064E-818.51
ATTAGAG609.220317E-418.53
GAACCGG609.220317E-418.532
CGTTGCC500.0070258918.49999826
AATTAGG500.0070258918.4999982
GTGAGAC500.0070258918.4999983
TATACAC500.0070258918.4999983
CTCGGAG1054.7750655E-717.61904712
TACCTTC1054.7750655E-717.6190477