##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364368
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79186426909059	33.0	33.0	33.0	33.0	33.0
2	31.283858626443596	33.0	33.0	33.0	27.0	33.0
3	31.729007486936283	33.0	33.0	33.0	27.0	33.0
4	31.850788214113205	33.0	33.0	33.0	27.0	33.0
5	31.975294208053395	33.0	33.0	33.0	33.0	33.0
6	34.87650122952619	37.0	37.0	37.0	27.0	37.0
7	35.065469525315066	37.0	37.0	37.0	33.0	37.0
8	35.22513502832302	37.0	37.0	37.0	33.0	37.0
9	35.311964826768545	37.0	37.0	37.0	33.0	37.0
10	35.356214047336756	37.0	37.0	37.0	33.0	37.0
11	35.386318227725816	37.0	37.0	37.0	33.0	37.0
12	35.343916040925656	37.0	37.0	37.0	33.0	37.0
13	35.383269112545555	37.0	37.0	37.0	33.0	37.0
14	35.33111305054231	37.0	37.0	37.0	33.0	37.0
15	35.36471644052167	37.0	37.0	37.0	33.0	37.0
16	35.368627321828484	37.0	37.0	37.0	33.0	37.0
17	35.32948283054495	37.0	37.0	37.0	33.0	37.0
18	35.34323815483248	37.0	37.0	37.0	33.0	37.0
19	35.34292528432793	37.0	37.0	37.0	33.0	37.0
20	35.34159695692267	37.0	37.0	37.0	33.0	37.0
21	35.33797699029552	37.0	37.0	37.0	33.0	37.0
22	35.34960808852588	37.0	37.0	37.0	33.0	37.0
23	35.334730272691345	37.0	37.0	37.0	33.0	37.0
24	35.34050465463488	37.0	37.0	37.0	33.0	37.0
25	35.34031254116717	37.0	37.0	37.0	33.0	37.0
26	35.29753984982216	37.0	37.0	37.0	33.0	37.0
27	35.248520726298686	37.0	37.0	37.0	33.0	37.0
28	35.24114905809511	37.0	37.0	37.0	33.0	37.0
29	35.27037500548896	37.0	37.0	37.0	33.0	37.0
30	35.224533987616915	37.0	37.0	37.0	33.0	37.0
31	35.21951159267554	37.0	37.0	37.0	33.0	37.0
32	35.262665766477845	37.0	37.0	37.0	33.0	37.0
33	35.24484861458745	37.0	37.0	37.0	33.0	37.0
34	35.169551113160324	37.0	37.0	37.0	33.0	37.0
35	35.174002656654814	37.0	37.0	37.0	33.0	37.0
36	35.15495323409301	37.0	37.0	37.0	33.0	37.0
37	35.20084914152725	37.0	37.0	37.0	33.0	37.0
38	35.05210940587538	37.0	37.0	37.0	27.0	37.0
39	35.11653602950863	37.0	37.0	37.0	33.0	37.0
40	35.165826856364994	37.0	37.0	37.0	33.0	37.0
41	35.190189588547845	37.0	37.0	37.0	33.0	37.0
42	35.115449216177055	37.0	37.0	37.0	33.0	37.0
43	34.898448820972206	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	9.0
16	18.0
17	13.0
18	17.0
19	13.0
20	27.0
21	67.0
22	164.0
23	392.0
24	859.0
25	1615.0
26	2597.0
27	4036.0
28	5750.0
29	7843.0
30	10377.0
31	13725.0
32	17225.0
33	23071.0
34	32950.0
35	61847.0
36	181752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89025380933562	20.513876081324376	13.354630483467265	25.241239625872748
2	15.825209678127608	23.34013963904624	37.941586527905855	22.8930641549203
3	17.718350678434987	26.642570148860496	30.995861327010054	24.643217845694462
4	12.98906599920959	18.036984587010934	38.62605936855047	30.347890045228997
5	13.516005796337769	37.56833750494006	35.857155403328505	13.058501295393668
6	29.60770429895051	39.322607912879285	17.758145611030606	13.311542177139597
7	26.05058622052431	32.525633425547795	23.866530540552407	17.55724981337549
8	24.089931058709876	36.471369604356035	21.0759451982611	18.36275413867299
9	25.596649541123263	15.923461994467134	19.96580380274887	38.51408466166074
10	14.454342862161331	28.68802968427524	34.54035480613006	22.317272647433363
11	33.25099899003205	23.731502217538313	24.673407104904932	18.3440916875247
12	22.203376805866597	25.8184033724147	30.356123479559127	21.622096342159576
13	27.170333289421684	22.269518728318623	27.061926404075	23.49822157818469
14	21.68741492117859	21.26339305317701	27.429960918631714	29.619231107012688
15	23.577262547753918	28.983061081104815	24.59491503095771	22.84476134018355
16	23.2539630263909	28.79314319588987	24.933034734114962	23.019859043604267
17	22.447635357660385	28.07271769200369	26.289081368287004	23.190565582048915
18	22.20310235805559	28.142152988187767	27.529859921837264	22.124884731919376
19	24.361085495982085	26.95351951872832	27.454112326000086	21.231282659289512
20	24.04766609581522	26.473510297281873	27.722522285162256	21.756301321740658
21	22.492095903043076	27.68300180037764	26.82507794317833	22.999824353400957
22	22.338130681069686	27.647872480569095	26.870636279805034	23.143360558556182
23	22.2681464892636	27.706055416501997	27.39812497255522	22.62767312167918
24	23.351940894919423	27.80348438940851	26.140056206911694	22.704518508760373
25	23.266587625697095	27.073727659948183	27.19475914460106	22.464925569753653
26	23.053341676546786	27.30289158213674	26.88545646159926	22.758310279717207
27	22.962499451104378	27.218636104158435	26.54431783252097	23.27454661221622
28	22.269244280507618	27.486771615509593	27.499670662626798	22.74431344135599
29	23.578360338997935	27.446153339480965	26.659311465331754	22.316174856189345
30	22.722357616475648	27.734597988846442	26.92497694638388	22.61806744829403
31	23.207855794142183	27.14837746454134	27.054516313177885	22.589250428138584
32	22.258266368067446	27.183506784349888	27.261724410486103	23.29650243709656
33	22.36090984938304	26.93677820225706	27.704134281824967	22.998177666534932
34	22.638376586308347	27.082784437711325	27.34872436657445	22.930114609405873
35	23.024799104202344	27.45877793878716	26.88545646159926	22.630966495411233
36	23.13265709392702	27.534799982435338	26.568743687700348	22.76379923593729
37	23.36017432924955	26.467746893250784	26.88737759627629	23.284701181223376
38	23.47050234927326	27.05863303034295	26.80284767048698	22.668016949896806
39	22.7964585254468	27.01746585869231	27.315241733631936	22.870833882228954
40	22.7835594783296	27.159355376981516	27.293011460940587	22.764073683748297
41	22.3002568831511	26.853894963333776	27.720052254863216	23.125795898651912
42	22.793439599525755	27.046282878847762	27.06494532999605	23.095332191630437
43	22.7964585254468	26.39474377552365	27.434352083607784	23.37444561542177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	9.5
2	17.0
3	53.5
4	90.0
5	90.0
6	114.5
7	139.0
8	124.5
9	110.0
10	162.5
11	215.0
12	215.0
13	323.0
14	431.0
15	809.0
16	1187.0
17	1400.5
18	1614.0
19	1614.0
20	1743.0
21	1872.0
22	2461.5
23	3051.0
24	3988.5
25	4926.0
26	4926.0
27	6273.5
28	7621.0
29	9931.5
30	12242.0
31	13848.5
32	15455.0
33	15455.0
34	17360.5
35	19266.0
36	21089.5
37	22913.0
38	25071.0
39	27229.0
40	27229.0
41	29039.0
42	30849.0
43	29559.5
44	28270.0
45	28325.5
46	28381.0
47	28381.0
48	27921.0
49	27461.0
50	27824.5
51	28188.0
52	28049.5
53	27911.0
54	27911.0
55	24340.5
56	20770.0
57	18273.0
58	15776.0
59	13789.5
60	11803.0
61	11803.0
62	10365.0
63	8927.0
64	7419.5
65	5912.0
66	5120.0
67	4328.0
68	4328.0
69	3533.0
70	2738.0
71	2322.0
72	1906.0
73	1559.0
74	1212.0
75	1212.0
76	939.5
77	667.0
78	503.5
79	340.0
80	284.5
81	229.0
82	229.0
83	182.0
84	135.0
85	119.0
86	103.0
87	80.0
88	57.0
89	57.0
90	38.0
91	19.0
92	12.5
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	364368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.164374596528326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.46093689911075	53.22196944757953
2	6.102043606751278	7.342512747218692
3	1.765639298986357	3.1868575255970053
4	0.7870954127370586	1.8942041302048584
5	0.5318770464321698	1.6000024930420076
6	0.354230010934663	1.2787216242723234
7	0.2561046931849812	1.0785865087697113
8	0.18652107903532175	0.8977539255383811
9	0.15859864993308923	0.8587789726570239
>10	1.1700618604857786	14.28071016066953
>50	0.15601663078399716	6.552098340107494
>100	0.06857368137703103	7.012846835138889
>500	0.002301130247551377	0.7949572892045546
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.19513239362402846	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	562	0.1542396697843938	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	559	0.15341632635138103	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	536	0.14710402669828304	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	503	0.13804724893514247	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	478	0.131186053660036	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	456	0.12514820181794228	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	383	0.10511351161463137	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	379	0.10401572037061432	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	373	0.10236903350458876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	2.7444781100425945E-4	0.0	0.0
3	0.0	0.0	2.7444781100425945E-4	0.0	0.0
4	0.0	0.0	5.488956220085189E-4	0.0	0.0
5	0.0	0.0	5.488956220085189E-4	0.0	0.0
6	0.0	0.0	8.233434330127783E-4	0.0	0.0
7	0.0	0.0	0.0021955824880340756	0.0	0.0
8	0.0	0.0	0.0027444781100425942	0.0	0.0
9	0.0	0.0	0.0032933737320511134	0.0	0.0
10	2.7444781100425945E-4	0.0	0.0035678215430553725	2.7444781100425945E-4	0.0
11	2.7444781100425945E-4	0.0	0.004391164976068151	2.7444781100425945E-4	0.0
12	2.7444781100425945E-4	0.0	0.004940060598076669	2.7444781100425945E-4	0.0
13	2.7444781100425945E-4	0.0	0.005763404031089448	2.7444781100425945E-4	0.0
14	2.7444781100425945E-4	0.0	0.006037851842093708	2.7444781100425945E-4	0.0
15	2.7444781100425945E-4	0.0	0.006586747464102227	2.7444781100425945E-4	0.0
16	2.7444781100425945E-4	0.0	0.006586747464102227	5.488956220085189E-4	0.0
17	2.7444781100425945E-4	0.0	0.006586747464102227	5.488956220085189E-4	0.0
18	2.7444781100425945E-4	0.0	0.006861195275106486	5.488956220085189E-4	0.0
19	2.7444781100425945E-4	0.0	0.006861195275106486	5.488956220085189E-4	0.0
20	2.7444781100425945E-4	0.0	0.006861195275106486	5.488956220085189E-4	0.0
21	2.7444781100425945E-4	0.0	0.007135643086110745	5.488956220085189E-4	0.0
22	2.7444781100425945E-4	0.0	0.007135643086110745	5.488956220085189E-4	0.0
23	2.7444781100425945E-4	0.0	0.007135643086110745	8.233434330127783E-4	0.0
24	2.7444781100425945E-4	0.0	0.007135643086110745	0.0013722390550212971	0.0
25	2.7444781100425945E-4	0.0	0.007135643086110745	0.0013722390550212971	0.0
26	2.7444781100425945E-4	0.0	0.007135643086110745	0.001921134677029816	0.0
27	2.7444781100425945E-4	0.0	0.007135643086110745	0.0024700302990383347	0.0
28	2.7444781100425945E-4	0.0	0.007135643086110745	0.013722390550212972	0.0
29	2.7444781100425945E-4	0.0	0.007135643086110745	0.02854257234444298	0.0
30	2.7444781100425945E-4	2.7444781100425945E-4	0.007135643086110745	0.04583278443771133	0.0
31	2.7444781100425945E-4	2.7444781100425945E-4	0.007135643086110745	0.1103280200237123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	160	0.0	26.593752	1
GTATCAA	455	0.0	23.175823	1
CCGTACA	60	3.7173006E-5	21.583334	17
GCTCGGA	80	6.9332964E-7	20.8125	11
AACCGGG	45	0.0038208703	20.555555	33
CGAACCG	45	0.0038208703	20.555555	31
ACGTTGC	45	0.0038208703	20.555555	25
ATTATAC	45	0.0038208703	20.555555	3
CGCAATA	45	0.0038208703	20.555555	34
ATACTAC	55	5.133834E-4	20.181818	3
GCCCCGT	65	6.885605E-5	19.923077	14
CCCCTAT	110	3.830064E-8	18.5	1
ATTAGAG	60	9.220317E-4	18.5	3
GAACCGG	60	9.220317E-4	18.5	32
CGTTGCC	50	0.00702589	18.499998	26
AATTAGG	50	0.00702589	18.499998	2
GTGAGAC	50	0.00702589	18.499998	3
TATACAC	50	0.00702589	18.499998	3
CTCGGAG	105	4.7750655E-7	17.619047	12
TACCTTC	105	4.7750655E-7	17.619047	7
>>END_MODULE