FastQCFastQC Report
Fri 10 Feb 2017
ERR1632807.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632807.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240646
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8650.35944914937293787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.2522377267854026No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5690.2364468971019672No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG4440.18450337840645598No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC4130.17162138576996916No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC4100.1703747413212769No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3600.14959733384307242No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC3520.14627294864655968No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT3480.1446107560483033No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3020.12549554116835518No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA2990.12424889671966291No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC2970.12341780042053473No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT2890.120093415224022No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA2750.11427574113012474No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC2730.11344464483099656No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA2430.10097820034407387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA850.034.823531
ATCCGTA250.005487817329.59999812
CGGAGTT250.005487817329.59999824
AATACTG250.005487817329.5999985
AGCTTAA556.227219E-726.90909410
GTATCAA4100.026.621951
CTCCTAA451.3189683E-424.6666681
GTACTGC751.3622412E-824.6666666
GTCGCCC551.8945639E-523.54545637
CCGAGGT551.8945639E-523.54545632
GCTCCGA551.8945639E-523.54545629
TATTCCG400.00192677623.1250025
CTGTTGG400.00192677623.1250021
GCTCGGA652.6682337E-622.7692311
TGCTCCG502.693507E-422.19999928
TGCTCGG705.0743092E-621.14285710
GTCGCCA450.003817152520.55555712
ATTACCC450.003817152520.5555573
TACTGCT909.400355E-820.5555577
CAGCTTA450.003817152520.5555579
Produced by FastQC (version 0.11.5)