##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632807.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240646
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77496821056656	33.0	33.0	33.0	27.0	33.0
2	31.29488127789367	33.0	33.0	33.0	27.0	33.0
3	31.722970670611605	33.0	33.0	33.0	27.0	33.0
4	31.82732312193014	33.0	33.0	33.0	27.0	33.0
5	31.94851358426901	33.0	33.0	33.0	33.0	33.0
6	34.86045893137638	37.0	37.0	37.0	27.0	37.0
7	34.98637833165729	37.0	37.0	37.0	33.0	37.0
8	35.195561114666354	37.0	37.0	37.0	33.0	37.0
9	35.23600641606343	37.0	37.0	37.0	33.0	37.0
10	35.31053913216925	37.0	37.0	37.0	33.0	37.0
11	35.34075779360555	37.0	37.0	37.0	33.0	37.0
12	35.28111001221711	37.0	37.0	37.0	33.0	37.0
13	35.34082428130947	37.0	37.0	37.0	33.0	37.0
14	35.27932315517399	37.0	37.0	37.0	33.0	37.0
15	35.32366629821397	37.0	37.0	37.0	33.0	37.0
16	35.30841983660647	37.0	37.0	37.0	33.0	37.0
17	35.280544866733706	37.0	37.0	37.0	33.0	37.0
18	35.24401818438702	37.0	37.0	37.0	33.0	37.0
19	35.29016480639612	37.0	37.0	37.0	33.0	37.0
20	35.21311802398544	37.0	37.0	37.0	33.0	37.0
21	35.27041380284734	37.0	37.0	37.0	33.0	37.0
22	35.294253800187825	37.0	37.0	37.0	33.0	37.0
23	35.26037000407237	37.0	37.0	37.0	33.0	37.0
24	35.2669273538725	37.0	37.0	37.0	33.0	37.0
25	35.27259958611404	37.0	37.0	37.0	33.0	37.0
26	35.22016987608354	37.0	37.0	37.0	33.0	37.0
27	35.16337275500112	37.0	37.0	37.0	33.0	37.0
28	35.17448866800196	37.0	37.0	37.0	33.0	37.0
29	35.21415689435935	37.0	37.0	37.0	33.0	37.0
30	35.16877903642695	37.0	37.0	37.0	33.0	37.0
31	35.160384963805754	37.0	37.0	37.0	33.0	37.0
32	35.188384598123385	37.0	37.0	37.0	33.0	37.0
33	35.175727001487665	37.0	37.0	37.0	33.0	37.0
34	35.10329695901864	37.0	37.0	37.0	33.0	37.0
35	35.09868853003998	37.0	37.0	37.0	33.0	37.0
36	35.06655003615269	37.0	37.0	37.0	33.0	37.0
37	35.105478586803855	37.0	37.0	37.0	33.0	37.0
38	34.939500344904964	37.0	37.0	37.0	27.0	37.0
39	35.00059838933537	37.0	37.0	37.0	27.0	37.0
40	35.04145923888201	37.0	37.0	37.0	27.0	37.0
41	35.07234277735761	37.0	37.0	37.0	33.0	37.0
42	34.95690350140871	37.0	37.0	37.0	27.0	37.0
43	34.786990018533444	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	7.0
17	8.0
18	6.0
19	4.0
20	20.0
21	36.0
22	117.0
23	303.0
24	612.0
25	1126.0
26	1908.0
27	2808.0
28	3937.0
29	5442.0
30	7089.0
31	9479.0
32	11845.0
33	15485.0
34	22481.0
35	41506.0
36	116418.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46400106380326	21.034631782784672	14.139441337067726	24.36192581634434
2	15.523216675116144	23.539556028357005	38.82092368042685	22.116303616099998
3	17.847793023777665	26.818230928417673	31.579997174272584	23.753978873532073
4	13.059431696350657	18.30946701794337	38.777706672872185	29.85339461283379
5	13.334524571362083	37.6943726469586	35.666082128936274	13.305020652743032
6	28.735570090506386	39.77751552072339	18.173998321185476	13.312916067584752
7	25.43902662001446	32.921386601065464	23.932664577844633	17.70692220107544
8	23.458108591042446	36.880729370112114	21.217472968592872	18.44368907025257
9	25.14315633752483	16.52718100446299	20.309500261795336	38.02016239621685
10	14.463153345578153	28.874363172460793	34.309317420609524	22.35316606135153
11	32.55902861464558	24.351121564455674	24.933304521994966	18.156545298903783
12	21.926813659898773	26.083541799988364	30.490845474265104	21.49879906584776
13	27.084597292288258	22.591690699201315	27.446539730558577	22.877172277951846
14	21.546171554898066	21.825419911405135	27.718308220373494	28.910100313323305
15	23.401178494552163	29.011494061816943	25.161856004255213	22.42547143937568
16	22.597923921444778	29.440755300316646	25.05215129277029	22.90916948546828
17	22.06352900110536	28.448010771008036	26.435095534519586	23.05336469336702
18	21.94011120068482	28.394820607863835	27.823441902213208	21.841626289238132
19	24.228950408483833	27.04387357363098	28.23275682953384	20.494419188351355
20	23.93017128894725	26.381489823225817	28.369472170740423	21.31886671708651
21	22.031947341738487	28.07152414750297	27.256634226207794	22.639894284550753
22	21.812953466918213	27.660547027584087	27.48726344921586	23.039236056281844
23	21.7610099482227	28.1579581626123	27.98135019904756	22.099681690117436
24	22.882989952045744	27.861256783823542	26.896769528685287	22.358983735445427
25	23.053780241516584	27.053015632921383	27.873723228310464	22.019480897251565
26	22.66108724017852	27.483107967720223	27.52175394562968	22.33405084647158
27	22.614961395576906	27.181004462987126	27.242921137272173	22.961113004163792
28	21.797162637234777	27.777731605761158	28.092301554981177	22.33280420202289
29	22.95612642636902	28.103936903168968	26.902587202779188	22.03734946768282
30	22.18237577188069	28.188293177530483	27.636445234909367	21.992885815679465
31	22.895040848383104	27.357196878402302	27.717892672223932	22.02986960099067
32	21.814615659516466	27.44238424906294	28.004205347273587	22.738794744147004
33	22.070177771498383	27.36966332288922	28.027891591798742	22.53226731381365
34	21.87196130415631	27.56039992353914	27.7008551980918	22.866783574212743
35	22.45871529134081	28.016671791760512	27.538375871612242	21.986237045286437
36	22.608728173333446	27.82967512445667	27.3247841227363	22.236812579473582
37	23.271527471888167	26.685255520557167	27.251232100263458	22.79198490729121
38	23.24867232366214	27.424931226781247	27.11908778870208	22.207308660854533
39	22.36438586138976	27.185159944482766	28.12803869584369	22.32241549828379
40	22.56966664727442	27.183082203734948	27.71207499813003	22.5351761508606
41	22.05064700846887	27.109945729411667	28.111832318010688	22.727574944108774
42	22.738379195997442	27.163966988854998	27.527571619723577	22.570082195423986
43	22.396798617055758	26.647440638946833	28.034124814042205	22.921635929955205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	8.0
2	14.0
3	48.5
4	83.0
5	83.0
6	115.5
7	148.0
8	129.0
9	110.0
10	157.0
11	204.0
12	204.0
13	333.0
14	462.0
15	914.0
16	1366.0
17	1594.5
18	1823.0
19	1823.0
20	1901.0
21	1979.0
22	2430.5
23	2882.0
24	3462.0
25	4042.0
26	4042.0
27	4850.5
28	5659.0
29	7317.5
30	8976.0
31	9715.5
32	10455.0
33	10455.0
34	11400.5
35	12346.0
36	13513.0
37	14680.0
38	16096.0
39	17512.0
40	17512.0
41	18733.5
42	19955.0
43	18872.5
44	17790.0
45	18164.0
46	18538.0
47	18538.0
48	18284.0
49	18030.0
50	18283.5
51	18537.0
52	18725.0
53	18913.0
54	18913.0
55	16058.5
56	13204.0
57	11779.0
58	10354.0
59	8747.5
60	7141.0
61	7141.0
62	6255.5
63	5370.0
64	4467.0
65	3564.0
66	2963.0
67	2362.0
68	2362.0
69	1965.5
70	1569.0
71	1306.5
72	1044.0
73	838.5
74	633.0
75	633.0
76	510.0
77	387.0
78	315.5
79	244.0
80	180.0
81	116.0
82	116.0
83	87.0
84	58.0
85	56.5
86	55.0
87	40.0
88	25.0
89	25.0
90	17.0
91	9.0
92	6.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	240646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.013344147089406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.46928616419798	40.91108848477724
2	8.089911209340668	7.930272044456207
3	2.499975658989967	3.675965020002656
4	1.2518224989529099	2.4542402780899373
5	0.8004835756456031	1.961718848860531
6	0.5509917618736946	1.6203569308155914
7	0.406843265901164	1.3958524303877684
8	0.3065771554848757	1.2021097303532762
9	0.25100561901853485	1.1072362309027795
>10	2.015947326942449	20.00673268150338
>50	0.26092212733477627	8.937964644955553
>100	0.09325733828695427	7.804003840209546
>500	0.002976298030434711	0.9924588346855059
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	865	0.35944914937293787	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	607	0.2522377267854026	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	569	0.2364468971019672	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	444	0.18450337840645598	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	413	0.17162138576996916	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	410	0.1703747413212769	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	360	0.14959733384307242	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	352	0.14627294864655968	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	348	0.1446107560483033	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	302	0.12549554116835518	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	299	0.12424889671966291	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	297	0.12341780042053473	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	289	0.120093415224022	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	275	0.11427574113012474	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	273	0.11344464483099656	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	243	0.10097820034407387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.00124664444869227	0.0	0.0
8	0.0	0.0	0.00249328889738454	0.0	0.0
9	0.0	0.0	0.00249328889738454	4.1554814956408997E-4	0.0
10	0.0	0.0	0.0033243851965127197	0.00124664444869227	0.0
11	0.0	0.0	0.00457102964520499	0.00124664444869227	0.0
12	0.0	0.0	0.00457102964520499	0.00124664444869227	0.0
13	0.0	0.0	0.00498657779476908	0.00124664444869227	0.0
14	0.0	0.0	0.00581767409389726	0.0016621925982563599	0.0
15	0.0	0.0	0.0066487703930254395	0.0016621925982563599	0.0
16	0.0	0.0	0.00747986669215362	0.0016621925982563599	0.0
17	0.0	0.0	0.00747986669215362	0.0016621925982563599	0.0
18	0.0	0.0	0.00789541484171771	0.0016621925982563599	0.0
19	0.0	0.0	0.00789541484171771	0.0016621925982563599	0.0
20	0.0	0.0	0.00789541484171771	0.00207774074782045	0.0
21	0.0	0.0	0.00789541484171771	0.00207774074782045	0.0
22	0.0	0.0	0.00789541484171771	0.00207774074782045	0.0
23	0.0	0.0	0.00789541484171771	0.00249328889738454	0.0
24	0.0	0.0	0.00789541484171771	0.00290883704694863	0.0
25	0.0	0.0	0.00789541484171771	0.00290883704694863	0.0
26	0.0	0.0	0.00789541484171771	0.0033243851965127197	0.0
27	0.0	0.0	0.00789541484171771	0.00373993334607681	0.0
28	0.0	0.0	0.00789541484171771	0.00789541484171771	0.0
29	0.0	0.0	0.00789541484171771	0.02119295562776859	0.0
30	0.0	0.0	0.00789541484171771	0.03490604456338356	0.0
31	0.0	0.0	0.00789541484171771	0.08269408176325391	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	85	0.0	34.82353	1
ATCCGTA	25	0.0054878173	29.599998	12
CGGAGTT	25	0.0054878173	29.599998	24
AATACTG	25	0.0054878173	29.599998	5
AGCTTAA	55	6.227219E-7	26.909094	10
GTATCAA	410	0.0	26.62195	1
CTCCTAA	45	1.3189683E-4	24.666668	1
GTACTGC	75	1.3622412E-8	24.666666	6
GTCGCCC	55	1.8945639E-5	23.545456	37
CCGAGGT	55	1.8945639E-5	23.545456	32
GCTCCGA	55	1.8945639E-5	23.545456	29
TATTCCG	40	0.001926776	23.125002	5
CTGTTGG	40	0.001926776	23.125002	1
GCTCGGA	65	2.6682337E-6	22.76923	11
TGCTCCG	50	2.693507E-4	22.199999	28
TGCTCGG	70	5.0743092E-6	21.142857	10
GTCGCCA	45	0.0038171525	20.555557	12
ATTACCC	45	0.0038171525	20.555557	3
TACTGCT	90	9.400355E-8	20.555557	7
CAGCTTA	45	0.0038171525	20.555557	9
>>END_MODULE