FastQCFastQC Report
Fri 10 Feb 2017
ERR1632806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308592
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89312.8941126147145746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68022.2042049048581944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35601.153626795250687No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG20780.6733810338570022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19490.6315782651526936No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG15980.517835847980505No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG15850.5136231658630166No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15340.4970964898636387No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13680.44330377974801677No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13470.4364986778659201No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10890.35289314045730286No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9750.3159511588116348No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9210.29845232540052885No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC9030.2926193809301602No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.29099911857728No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA7840.25405713693161197No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7730.25049255975527557No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7450.24141909057914657No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7370.23882667081453832No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7250.23493804116762584No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7160.23202156893244152No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC7140.2313734639912895No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7020.22748483434437702No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC6920.22424430963861672No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6780.21970757505055222No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6670.2161429978742158No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6360.2060973712863587No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.20577331881578265No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6300.20415305646290247No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6230.20188468916887023No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6150.19929226940426192No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6070.19669984963965365No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.18697827552237256No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5760.18665422305179655No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5720.1853580131694924No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5720.1853580131694924No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5710.18503396069891637No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5710.18503396069891637No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.1843858557577643No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5680.18406180328718824No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT5680.18406180328718824No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG5590.18114533105200392No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5480.17758075387566755No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5330.17271996681702703No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5140.16656296987608232No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5090.16494270752320214No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5080.16461865505262613No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4980.16137813034686577No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4940.16008192046456163No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4930.1597578679939856No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4870.15781355317052936No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4730.15327681858246486No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4680.15165655622958468No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC4640.15036034634728054No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4630.1500362938767045No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4610.14938818893555245No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4580.14841603152382432No Hit
TATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCGG4500.14582361175921604No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4480.145175506818064No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4410.14290713952403172No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4380.14193498211230363No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4340.1406387722299995No Hit
CTTCCTGAGCCTTTCGGCCCAGGCGGCGGACGGCGACTCGCCG4250.13772229999481517No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4230.13707419505366308No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4220.13675014258308704No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA4140.13415772281847876No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG4120.1335096178773267No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT4070.13188935552444653No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC4060.13156530305387049No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4050.13124125058329444No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4010.1299450407009903No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3990.12929693575983822No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3940.12767667340695807No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3870.1254083061129258No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3800.12313993881889357No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3770.12216778140716544No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA3730.12087157152486132No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3690.11957536164255715No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3650.11827915176025301No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3650.11827915176025301No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3620.11730699434852493No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3590.1163348369367968No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3590.1163348369367968No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3580.11601078446622076No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3560.11536267952506869No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3560.11536267952506869No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3540.11471457458391662No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA3530.11439052211334058No Hit
GTTTCATACTAGTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.11050189246642816No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3390.10985378752527611No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3370.10920568258412401No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3360.10888163011354798No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3310.1072613677606678No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3290.10661326281951573No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3280.10628921034893969No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC3240.10499300046663555No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.10466894799605952No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3220.10434489552548348No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA3160.10240058070202727No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3120.10110437081972312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTTG200.001839850237.032
GTCAATT200.001839850237.026
CATTTGA200.001839850237.033
CTCTTTG303.5934316E-430.83333236
GAATCAA551.8464561E-830.2727281
CATTGTA250.0054901929.64
CCGTCAA250.0054901929.624
TCAATTC250.0054901929.627
CAGGATT250.0054901929.636
AATTCAT250.0054901929.629
GGGCCCC250.0054901929.618
TTCATTT250.0054901929.631
GGTATCA11600.028.866381
TGCGGTA358.854638E-426.428575
TATGCGG358.854638E-426.428573
GCGTCAC358.854638E-426.4285726
GCCTAGT509.062971E-625.9000021
GTATTAG451.3201493E-424.6666689
CCTAGTT451.3201493E-424.6666682
ACCCTGG451.3201493E-424.66666810
Produced by FastQC (version 0.11.5)