FastQCFastQC Report
Fri 10 Feb 2017
ERR1632805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247325
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15300.6186192257151522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.48842615991104826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5940.24016981704235318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.14555746487415344No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA3450.13949257050439703No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2790.11280703527746891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2900.032.534481
GCCGTCC502.7062015E-729.627
GTCCTAG358.849645E-426.428571
TCGCCGT601.330327E-624.66666625
CGAGTCG601.330327E-624.66666621
CGCCGTC601.330327E-624.66666626
GCGAGTC601.330327E-624.66666620
ACCTAGG551.8948414E-523.5454541
TGGGCGC551.8948414E-523.54545412
GCCGCTC400.00192692723.12500227
CGGGGAA400.00192692723.12500217
TTCGCCG400.00192692723.12500224
ATGGGCG652.6687321E-622.7692311
GGCGAGT652.6687321E-622.7692319
CGCGGCT502.6938005E-422.210
TACTGAG502.6938005E-422.25
GTATCAA8250.022.1999991
GCGCAAG603.7110854E-521.58333215
CGCAAGC603.7110854E-521.58333216
GTCCGCC705.075255E-621.14285730
Produced by FastQC (version 0.11.5)